Estrategias combinadas para la obtención de un vino Tannat con un contenido moderado de alcohol

Resumen En este trabajo se buscó a través de la combinación de 2 estrategias reducir el contenido de alcohol en vinos de la variedad Tannat. La primera estrategia consistió en el screening dentro de clones uruguayos de Tannat Con este fin se muestrearon durante tres vendimias consecutivas 10 clones uruguayos de Tannat y se utilizó como referencia el clon francés 717 en viñedos. De los clones estudiados 4 presentaron durante la vendimia 2023 un contenido significativamente inferior de alcohol que el clon 717, manteniendo los componentes responsables de su calidad sensorial (aromas glicosidados, antocianos totales). Mientras que la segunda estrategia fue realizar un screening de levaduras no-Saccharomyces nativas aisladas de viñedos uruguayos para utilizar en conjunto con la cepa Saccharomyces cerevisiae Lalvin BM4x4 Lalleman (utilizada como referencia para vinificar vino Tannat). Se estudió el comportamiento de 4 géneros de levaduras (Candida zemplinina, Metschnikowia pulcherrima, Metschnikowia fructicola y Hanseniaspora uvarum), en microvinificaciones en símil vino. Estas estrategias fueron seleccionada por que además de permitir la reducción de alcohol en vinos Tannat en forma conjunta le aportan una identidad propia de nuestro país

Improvement of verbal fluency in patients with diffuse brain injury over time

Ana Luiza Zaninotto,1 Vinícius Monteiro de Paula Guirado,2 Beatriz Baldivia,1 Monica Domiano Núñes,1 Robson Luis Oliveira Amorim,2 Manoel Jacobsen Teixeira,2 Mara Cristina Souza de Lucia,1 Almir Ferreira de Andrade,2 Wellingson Silva Paiva2 1Division of Psychology, Hospital das Clínicas, 2Division of Neurosurgery, University of São Paulo Medical School, São Paulo, Brazil Background: Diffuse axonal injury (DAI), a common cause of neurological sequelae in patients with traumatic brain injury (TBI), is considered one of the most prevalent forms of primary neuronal injury in patients with severe TBI. Cognitive deficits induced by DAI can persist over time, especially following moderate or severe injuries. The aim of the present study was to compare verbal fluency (VF) performance at 6 and 12 months after the trauma in a same group of patients with DAI. Methods: Eighteen patients with moderate to severe DAI and 17 healthy volunteers were enrolled. All DAI participants had sustained a TBI at least 6 months prior to the start of the study, were between 18 and 50 years of age, and had at least 4 years of education. The VF test was administered within an extensive neuropsychological test battery. We evaluated the same patients at 6 months (DAI1 group) and 12 months (DAI2 group) and compared the results of neuropsychological tests with a control group of healthy volunteers who were matched to patients for sex, age, and educational level. Results: In comparison to controls, the DAI1 group produced significantly fewer words. The DAI2 group produced significantly more semantic words than DAI1 (P<0.05) and demonstrated a trend towards the production of more clusters for letter A (P=0.09) and total words generated in a phonemic test (P=0.09). No significant differences were observed between DAI2 and the control group in the total number of words generated in phonetic FAS or semantic fluency scores. Conclusion: The present findings may be useful in the construction of a management plan for long-term TBI rehabilitation that considers the trauma of each patient. Further, our results suggest the VF test is a suitable instrument for the assessment of cognitive difficulties following TBI. Keywords: traumatic brain injury, neuropsychological tests, diffuse axonal injury, prognosis, executive functio

Effect of Polyphenols From Campomanesia adamantium on Platelet Aggregation and Inhibition of Cyclooxygenases: Molecular Docking and in Vitro Analysis

Campomanesia adamantium is a medicinal plant of the Brazilian Cerrado. Different parts of its fruits are used in popular medicine to treat gastrointestinal disorders, rheumatism, urinary tract infections and inflammations. Despite its widespread use by the local population, the mechanisms involving platelet aggregation and the inhibition of cyclooxygenase by C. adamantium are unknown. This study evaluated the chemical composition, antioxidant activities and potential benefits of the C. adamantium peel extract (CAPE) and its components in the platelet aggregation induced by arachidonic acid in platelet-rich plasma. Aspects of the pharmacological mechanism were investigated as follows: platelet viability, calcium mobilization, levels of the cyclic nucleotides cAMP and cGMP, thromboxane B2 levels, and the inhibitory effects on COX-1 and COX-2 were studied in vitro and using molecular docking in the catalytic domain of these proteins. The major CAPE constituents standing out from the chemical analysis are the flavonoids, namely those of the flavones and chalcones class. The results showed that CAPE, quercetin and myricetin significantly decreased arachidonic acid-induced platelet aggregation; the assays showed that CAPE and quercetin decreased the mobilization of calcium and thromboxane B2 levels in platelets and increased cAMP and cGMP levels. Moreover, CAPE inhibited the activity of COX-1 and COX-2, highlighting that quercetin could potentially prevent the access of arachidonic acid more to the catalytic site of COX-1 than COX-2. These results highlight CAPE’s potential as a promising therapeutic candidate for the prevention and treatment of diseases associated with platelet aggregation

Dementia in Parkinson's disease: a Brazilian sample

To determine the prevalence of dementia associated with Parkinson's disease (PD-D) in a Brazilian sample adopting clinical and diagnostic procedures recommended by the Movement Disorders Society (MDS). Sixty-seven patients were consecutively submitted to neurological, neuropsychological and functional examinations. PD-D was established according to MDS clinical criteria (Level II) and clinical procedures for PD-D (Level I) and prevalence rate was compared between the levels adopted. Ten patients (14.9%) were diagnosed as demented by Level I criteria whereas sixteen (23.8%) were diagnosed based on Level II criteria. Level I criteria had low sensitivity in detecting PD-D (31.25%), but greater specificity (90.19). The PD-D group had significantly worse performance on all neuropsychological tests, were older (p<0.001), had an older age of onset ofdisease (p<0.01), had lower educational level (p<0.02) and had higher scores on functional scales. Current age (p=0.046) and Hoehn & Yahr score (p=0.048) were predictors for developing PD-D

Diferenças quanto à escolaridade em adultos no desempenho no teste de cancelamento dos sinos Differences regarding education in adults in the bells test

O papel de variáveis sociodemográficas na cognição humana, em especial da escolaridade, vem sendo estudado em indivíduos saudáveis. Esta pesquisa objetivou verificar se há diferenças entre grupos de diferentes níveis educacionais no processamento atencional visual avaliado pelo Teste de Cancelamento dos Sinos. Participaram 124 adultos jovens, distribuídos em três grupos, 5-8, 9-11 e 12 ou mais anos de educação formal, emparelhados por idade. As variáveis de acurácia e tempo, assim como os resultados qualitativos de estratégias utilizadas, foram comparados entre grupos (ANCOVA One-Way e Qui-quadrado). Observou-se uma discrepância entre dados quantitativos e qualitativos. Os grupos de diferentes escolaridades não se diferenciaram entre si quanto a acurácia e tempo; houve, porém, diferenças significativas quanto à distribuição de participantes por coluna em que o primeiro sino foi cancelado e pelas estratégias de cancelamento utilizadas. Novos estudos são relevantes com grupos de diferentes idades e com populações clínicas neurológicas e/ou psiquiátricas.<br>The role of socio-demographic variables on human cognition, particularly education, has been studied in healthy individuals. This research aimed to verify if there are differences among educational groups regarding the visual attentional processing assessed by means of Bells Test. The sample was comprised of 124 young adults, divided into three groups, 5-8, 9-11 and 12 or more years of formal education, matched by age. Accuracy and time data were analyzed by one-way ANCOVA and the qualitative results on strategies were compared between groups by Chi-square. The results showed a discrepancy between the quantitative and qualitative analysis. There were no quantitative differences among educational groups regarding accuracy and time variables. However, significant differences were found in the distribution of participants by chosen column where the first bell was canceled and the used strategies of cancellation. Further studies are relevant including different age groups and clinical samples with neurological and/or psychiatric disorders