Métodos de esterilização de sementes de arroz para cultivo monoxênico com bactérias diazotróficas.

bitstream/CNPAB-2010/33274/1/cot015.pd

Herlyn-Werner-Wunderlich syndrome in a adolescent girl

Herlyn-WernerWunderlich syndrome (HWWS) is a rare congenital genitourinary anomaly with uterine anomalies, unilateral cervicovaginal obstruction, and ipsilateral renal anomalies resulting from the embryological arrest of Müllerian and mesonephric ducts. The onset of nonspecific symptoms occurs after menarche. Having two normal uteri and obstructed cervix or hemivagina, the patient will have regular menses through the non-obstructed vaginal side, coinciding with cyclic pelvic pain from the encumbered blood in the obstructed side. The diagnosis is often delayed until acute complications develop

Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium.

During a survey of the occurrence of Azospirillum spp. in cereal roots, we obtained 119 isolates which could not be identified as members of one of the three previously described Azospirillum species. These strains formed a very homogeneous group of N2-fixing, microaerobic, motile, vibrioid, gram-negative rod-shaped organisms which formed a veillike pellicle in semisolid medium similar to that of Azospirillum spp. However, the new isolates differed from Azospirillum spp. by their smaller cell width (0.6 to 0.7 μm), variable flagellation (one to three flagella on one or both poles), moist brownish colonies, and broader pH and oxygen tolerance for nitrogenase activity. Organic acids were the preferred carbon sources, but glucose, galactose, L-arabinose, mannitol, sorbitol, and glycerol were also used. The guanine-plus-cytosine content of the deoxyribonucleic acid was slightly lower than the guanine-plus-cytosine contents of Azospirillum spp. (66 to 67 mol%). Deoxyribonucleic acid hybridization experiments with 17 strains of the group showed 50 to 100% complementarity, while the levels of hybridization with the type strains of Azospirillum brasilense, Azospirillum lipoferum, and Azospirillum amazonense were 23, 15, and 6%, respectively. For these new isolates we propose a new genus, Herbaspirillum (the name refers to the habitat of the organisms, the roots of cereals, which are herbaceous seed-bearing plants). The type species is named Herbaspirillum seropedicae after the place where it was first isolated. The type strain is strain Z67, which has been deposited in the American Type Culture Collection as strain ATCC 3589

Bactérias diazotróficas em abacaxizeiros e bananeiras: técnicas de inoculação e monitoramento.

As bacterias diazotroficas tem sido isoladas de varias culturas, alem de leguminosas e gramineas, que tem importancia economica e social; deplantas de tuberculos como a mandioca foram isoladas bacterias do genero Burkholderia, entre outras(Balota, 1994); ja em plantas de batata-doce tem sido detectadas especies de Azospirillum(Hill et al.,1983) e Acetobacter diazophicus(Paula, 1992). Esta ultima especie também tem sido isolada de plantas de cafe(Jimenez-Salgado et al., 1998); na rizosfera de fruteiras tropicais tem sido isoladas especies de Azospirillum(Subba Rao, 1983; Ghai & Thomas, 1989). Especies deste genero e bacterias diazotroficas do tipo Herbaspirillum e Burkholderia foram tambem isoladas de raizes...bitstream/CNPAT-2010/4782/1/Dc-035.pd

Fixação biológica de nitrogênio: Bactérias fixadoras de nitrogênio de importância para a agricultura tropical.

Diazotrofos de vida livre. Diazotrofos associativos. Endófiticos facultativos. Endofíticos obrigatórios. Diazotrofos simbióticos.bitstream/CNPAB-2010/27316/1/doc091.pd

A NUMERICAL-EXPERIMENTAL STUDY OF THE AIR ENTRAINMENT FOR SELF ASPIRING BURNERS

This work presents an experimental and numerical study addressing the effects of air entrainment for self aspiring burners. Within this framework, the work focus on the analysis of four different burners tested with four different nozzles, resulting in a total of sixteen different configurations. The gas used for the tests was methane. Each nozzle diameter provided a different flow rate, which also resulted in a different entrainment, therefore a different oxygen concentration on the ports area, which was measured using an oxygen sensor. The level of oxygen decreased with the increase of the flow rate of the gas, and had a linear behavior with the nozzle diameter. The finite volume method with the realizable k-ε model was then applied to numerically investigate this phenomenon using the commercial software STARCCM+. The results shows that for the sixteen points tested the worst correlation had a relative error of 2.95%, while for the best one was of 0.04%

Ensaio de atividade de B-galactosidase em Gluconacetobacter diazotrophicus para estudos de expressão gênica.

Este Comunicado Técnico mostra a repetitibilidade de ensaios de atividade de ?-galactosidase, que foram padronizados com G. diazotrophicus, ilustrando sua utilidade em estudos de expressão gênica que envolvem fusões com o gene repórter lacZ. Os resultados mostrados neste Comunicado Técnico ilustram que o meio, as condições de cultivo e o ensaio de atividade de ?-galactosidase estabelecidos para G. diazotrophicus são reprodutíveis, e, portanto, válidos, sendo de grande valia para quantificar níveis de expressão gênica nessa importante bactéria constituinte do inoculante para cana-de-açúcar da Embrapa.bitstream/item/42760/1/COT123-09.pd