A high-throughput regeneration protocol for recalcitrant tropical Indian maize (Zea mays L) inbreds

Immature embryos from five select recalcitrant maize (Zea mays L) inbreds used as explants were evaluated for their ability to form callus, somatic embryos and subsequent regeneration into plants. The embryos were placed on N6 basal media with varying levels of 2,4-D (0.5, 1.0, 1.5, 2.0, and 2.5 mg l-1) and were regenerated on MS me¬dium supplemented with BAP (2 - 10 mg l-1), 2,4-D (0.25 mg l-1) and silver nitrate (0.85 mg l-1). Explants cultured on N6 medium supplemented with 2,4-D (2.0 mg l-1) were associated with the highest frequency of embryogenic calli and that of UMI 29 were highly embryogenic (78.67%). When synergism between dicamba and 2,4-D on Type II callus production in UMI 29 was sought to be investigated using 2,4-D (1 or 2 mg l-1) individually and in combina¬tion with dicamba (3.7 mg l-1) production of Type II callus with the greatest frequency of 83.33% was observed on N6 medium containing 3.7 mg l-1 dicamba + 1 mg l-1 2,4-D. The greatest percentage of shoot induction (82.67%) was observed on MS medium supplemented with BAP (10 mg l-1). Among the five genotypes tested, UMI 29 was associated with the highest percentage of callus initiation, shoot induction and mean number of developed shoots. The protocol described in this study can reliably be used to transform tropical maize inbreds as a routine

Spectroscopic studies on the structure and aggregation of calcium ionophore, A23187

Studies on the structure of calcium ionophore, A23187, using IR, UV, CD, fluorescence and NMR methods indicated that this ionophore forms a dimer in nonpolar solvents such as chloroform. When incorporated into a phospholipid vesicle, the spectra showed remarkable time dependent changes indicating further aggregation of the dimer into a stacked dimeric structure in dipalmitoylphosphatidylcholine vesicle. Results point to the possibility of A23187 transporting divalent cations across membranes by a pore rather than by a diffusive carrier model

Aggregation of calcium ionophore (A23187) in phospholipid vesicles

The circular dichroism studies on calcium ionophore, A23187, incorporated in Dipalmitoyl phosphatidyl choline (DPPC) vesicle showed interesting time dependent changes in the CD spectra. Analysis of the data indicated the possible aggregation of the observed dimeric structure of this molecule in non-polar solvents into a stacked dimeric pore in the phospholipid vesicle

Profunda Femoris Pseudoaneurysm following Total Hip Arthroplasty Revision

Vascular injuries following total hip arthroplasty (THA) are very rare, with pseudoaneurysm being a small subset. We report a case of profunda femoris artery (PFA) pseudoaneurysm in a 61-year-old male following a posterior approach revision left THA. Presentation involved continued blood transfusion requirements several weeks postoperatively. Diagnosis of the pseduoaneurysm was made by contrast CT of the lower extremity, with confirmation via IR angiography. Successful embolization was achieved with selective coiling and Gelfoam. Presenting complaints of such complications are often vague and therefore lead to delayed diagnosis. Causes of such complications are not completely understood, particularly with PFA injuries in THA. Possible mechanisms are discussed in this paper. Vascular complications following THA can be difficult to diagnose. High suspicion in the setting of continued postoperative pain or bleeding may allow prompt diagnosis and avoidance of serious limb-threatening complications

Conformation of polymerizable diacetylenic lecithin, DC8,9PC, by 1H-NMR spectroscopy

Diacetylenic phospholipid, 1,2 bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine (DC8,9PC), forms helices and tubules in addition to liposomes. The diacetylenic moiety responsible for the transformation is probed by 2-D NMR correlated spectroscopy. Chemical shift assignments and the analysis of 2D-COSY measurements were done on the lipid in chloroform-d solution. Based on this analysis, a model for the lipid is proposed. The geometry of the headgroup, glycerol backbone and acyl chains up to three methylenes from glycerol backbone [-(CH2)(3)-] is similar to that of dipalmitoyl phosphatidylcholine. The estimated torsional angle for methylene groups adjacent to diacetylenic moieties suggested an overall tilt of the diacetylenic lipid molecule from the bilayer axis of 25-30 degrees. This tilt could be negative or positive depending on the handedness of the resultant microstructures

Case Report Profunda Femoris Pseudoaneurysm following Total Hip Arthroplasty Revision

License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Vascular injuries following total hip arthroplasty (THA) are very rare, with pseudoaneurysm being a small subset. We report a case of profunda femoris artery (PFA) pseudoaneurysm in a 61-year-old male following a posterior approach revision left THA. Presentation involved continued blood transfusion requirements several weeks postoperatively. Diagnosis of the pseduoaneurysm was made by contrast CT of the lower extremity, with confirmation via IR angiography. Successful embolization was achieved with selective coiling and Gelfoam. Presenting complaints of such complications are often vague and therefore lead to delayed diagnosis. Causes of such complications are not completely understood, particularly with PFA injuries in THA. Possible mechanisms are discussed in this paper. Vascular complications following THA can be difficult to diagnose. High suspicion in the setting of continued postoperative pain or bleeding may allow prompt diagnosis and avoidance of serious limb-threatening complications. 1

Bilayers containing calcium ionophore A23187 form channels

For the first time, based on bilayer membrane conductance experiments, it has been shown that A23187, a carboxylic calcium ionophore, incorporated in lipid bilayers gives single channel currents similar to the well known gramicidin channel. The current characteristics indicate the possibility that the transmembrane ion transport by this important calcium ionophore is initially by a carrier mechanism but with time is by a channel or pore mechanism due to the aggregation of the molecule in a lipid matrix