Professional training for registered unemployed in public employment services in terms of system transformation

Professional training of registered unemployed as a mean of active employment policy at the labor market is one of the priorities of the State Employment Service. Professional training and skills retraining aimed at getting someone who has lost a job, a new profession or specialty on the basis of existing educational level and previous work experience, providing professional fulfillment of unemployed, the formation of their deep professional knowledge, skills and abilities. The purpose of research - analysis of professional training of registered unemployed in the state employment service and its efficiency. In the study were used the following general scientific and special methods: scientific generalization and systematization – to determine the benefits and risks of educational services centers of professional education in Ukraine; comparative analysis – for comparing scientific approaches to determine the role of professional training of registered unemployed in the labor market; system analysis – to identify factors influencing the amount of realized educational services. Conducted the analysis of professional training of registered unemployed by public employment services, benefits and risks that affect its efficiency. Considered the organization and features of professional training of registered unemployed by public employment in terms of transformation of the system; identified factors that influence the effectiveness of training unemployed centers, professional education state employment of Ukraine, namely: identification of professions that are needed in the labor market; forming the unemployed motivation to study; human resources; high level of material and technical equipment of the university

Aggregatibacter actinomycetemcomitans leukotoxin induces cytosol acidification in LFA-1 expressing immune cells

Studies have suggested that Aggregatibacter actinomycetemcomitans leukotoxin (LtxA) kills human lymphocyte function-associated antigen 1 (LFA-1; CD11a/CD18)-bearing immune cells through a lysosomal-mediated mechanism. Lysosomes are membrane-bound cellular organelles that contain an array of acid hydrolases that are capable of breaking down biomolecules. The lysosomal membrane bilayer confines the pH-sensitive enzymes within an optimal acidic (pH 4.8) environment thereby protecting the slightly basic cytosol (pH 6.8-7.5). In the current study, we have probed the effect of LtxA-induced cytolysis on lysosomal integrity in two different K562 erythroleukemia cell lines. K562-puro/LFA-1 cells were stably transfected with CD11a and CD18 cDNA to express LFA-1 on the cell surface while K562-puro, which does not express LFA-1, served as a control. Following treatment with 100 ng ml-1 LtxA cells were analyzed by live cell imaging in conjunction with time-lapse confocal microscopy and by flow cytometry. Using a pH-sensitive indicator (pHrodo®) we demonstrated that the toxin causes a decrease in the intracellular pH in K562-puro/LFA-1 cells that is noticeable within the first 15 min of treatment. This process correlated with the disappearance of lysosomes in the cytosol as determined by both acridine orange and LysoTracker® Red DND-99 staining. These changes were not observed in K562-puro cells or when heat inactivated toxin was added to K562-puro/LFA-1. Our results suggest that LtxA induces lysosomal damage, cytosol acidification, which is followed by cell death in K562-puro/LFA-1 cells. © 2016 John Wiley & Sons A/S

Organizational and methodological approaches to development of accounting policy for formation of integrated accounting of interrelated agricultural companies

The purpose of the article is to determine advantages and drawbacks of existing organizational and methodological approaches to development of accounting policy and develop optimal approach to formation of integrated accounting of interrelated agricultural enterprises. For this purpose, the article uses methods of problem and comparative analysis, method of optimization, and methods of graphical presentation of information. The authors conduct comparative analysis of existing organizational and methodological approaches to development of accounting policy of enterprise and develop optimal approach to formation of integrated accounting of interrelated agricultural enterprises. As a result of the research, the authors come to the conclusion that Russian and international approach to development of accounting policy differ from each other, but neither of these approaches is optimal for formation of integrated accounting of interrelated agricultural enterprises. The developed optimal approach to formation of integrated accounting allows unifying the process of development of accounting policy of enterprise and thus is the best for interrelated agricultural enterprises. This approach is oriented at consumers, due to which it is able not only to simplify the process of formation of corporate accounting of interrelated agricultural enterprises but to turn it into the factor of their competitiveness.peer-reviewe

Inhibition of LtxA Toxicity by Blocking Cholesterol Binding with Peptides

The leukotoxin (LtxA) produced by Aggregatibacter actinomycetemcomitans kills host immune cells, allowing the bacterium to establish an ecological niche in the upper aerodigestive tract of its human host. The interaction of LtxA with human immune cells is both complex and multifaceted, involving membrane lipids as well as cell-surface proteins. In the initial encounter with the host cell, LtxA associates with lymphocyte function-associated antigen-1, a cell surface adhesion glycoprotein. However, we have also demonstrated that the toxin associates strongly with the plasma membrane lipids, specifically cholesterol. This association with cholesterol is regulated by a cholesterol recognition amino acid consensus (CRAC) motif, with a sequence of 334LEEYSKR340, in the N-terminal region of the toxin. Here, we have demonstrated that removal of cholesterol from the plasma membrane or mutation of the LtxA CRAC motif inhibits the activity of the toxin in THP-1 cells. To inhibit LtxA activity, we designed a short peptide corresponding to the CRAC336 motif of LtxA (CRAC336WT). This peptide binds to cholesterol and thereby inhibits the toxicity of LtxA in THP-1 cells. Previously, we showed that this peptide inhibits LtxA toxicity against Jn.9 (Jurkat) cells, indicating that peptides derived from the cholesterol-binding site of LtxA may have a potential clinical applicability in controllinginfections of repeats-in-toxin-producing organisms. © 2016 John Wiley & Sons A/S

Conformation of surface exposed N-terminus part of bacteriorhodopsin studied by transferred NOE technique

AbstractInteraction of the monoclonal antibody A5 raised against native bacteriorhodopsin (BR) with the synthetic peptide pGlu1-Ala-Gln-Ile-Thr-Gly-Arg7-NH2, corresponding to the amino acid sequence 1–7 was studied by transferred nuclear Overhauser effect (TRNOE) spectroscopy. The denaturing reagents and the specially designed pulse sequences which eliminate broad signals from the TRNOE spectra were used to favour evaluation of the TRNOE peaks. On the basis of the data obtained, the conformation of peptide bound with A5 was calculated. A model of the mutual arrangement of bacteriorhodopsin N-terminus and the first transmembrane α-helical segment 8–32 was proposed

THIN FILMS OF A NEW ORGANIC SINGLE-COMPONENT FERROELECTRIC 2-METHYLBENZIMIDAZOLE

Subject of Research.We present results of structural and dielectric study of organic ferroelectric 2-methylbenzimidazole (MBI) thin films. Method. The films have been grown on substrates of leuco-sapphire, fused and crystalline silica, neodymium gallate, bismuth germanate, gold, aluminium, platinum. The films have been grown by two different methods: substrate covering by ethanol solution of MBI and subsequent ethanol evaporation; sublimation at the temperature near 375 K under atmospheric pressure. Crystallographic orientation studies have been performed by means of «DRON-3» X-ray diffractometer, block structure of the films has been determined by «LaboPol-3» polarizing microscope. Small-signal dielectric response has been received with the use of «MIT 9216A» digital LCR-meter, while strong-signal dielectric response has been studied by Sawyer-Tower circuit. Main Resuts. We have shown that the films obtained by evaporation are continuous and textured. Obtained film structure depends on the concentration of the solution. Films may consist of blocks that are splitted crystals like spherulite. Spontaneous polarization components in such films may be directed both perpendicularly and in the film plane. We have also obtained structures consisting of single-crystal blocks with spontaneous polarization components being allocated in the film plane. Block sizes vary from a few to hundreds of microns. Films obtained by sublimation are amorphous or dendritic. The dielectric properties of the films obtained by evaporation have been studied. We have shown that the dielectric constant and dielectric loss tangent increase under heating. The dielectric hysteresis loops are observed at the temperature equal to 291-379 K. The remnant polarization increases with temperature for constant amplitude of the external electric field, and achieves 4.5mC/cm2, while the coercive field remains constant. We propose that such behavior is explained by increase of the number of crystallites with switchable polarization due to decrease in the coercive field under heating. The remnant polarization decreases with frequency increase. Practical Relevance. Proposed method of ferroelectric films manufacture is characterized by low cost and convenience. Unlike many other ferroelectrics, the films contain no lead and rare metals. MBI films demonstrate the low value of the coercive fields. This paper may be useful for electronic components developers

Metabolic adverse effects of antipsychotics: the state of the problem and management options

Antipsychotic  drugs are widely used for many psychiatric disorders, such as schizophrenia, bipolar affective disorder, delusions and hallucinations  due to neurological  disorders, depression with severe psychotic  symptoms. Metabolic disorders including  weight gain, dyslipidemia and hyperglycemia  are one of the most common side effects of antipsychotic therapy. Psychiatric patients have higher risk of cardiovascular disease, so that the development of metabolic side effects is an important clinical problem that should be solved. Antipsychotic-induced weight gain may cause distress that leads to antipsychotics withdraw and repeated hospitalizations.Lifestyle changes, correction of the antipsychotic treatment, additional medications and their combination are the possible solutions of antipsychotic metabolic side effects. Lifestyle modification is a first-line therapy that should complement other options, when it feasible. At the same time, it can be extremely difficult for patients receiving antipsychotic to adhere dietary and physical activity recommendations. Replacing an antipsychotic with a milder drug is not always possible and may not be enough effective. Metformin seems to be the most well-studied, safe and effective agent that is prescribed to deal with antipsychotic-induced weight gain and associated metabolic disorders. Glucagon-like peptide type 1 receptor agonists and thiazolidinediones  are mentioned as alternative medications, but clinical data on their efficacy and safety in this patient group are extremely limited. Dyslipidemia can develop as an independent antipsychotic side effect even without an increase in body weight. The most effective treatment, as in the general population, is statin therapy. However, the joint appointment of statins and antipsychotic significantly  increases the risk of adverse reactions, such as myalgia, myopathy, increased creatine kinase levels, due to the competition of drugs for the cytochrome system.It is still unknown what scales should be used for cardiovascular risk stratification in patients taking antipsychotic and whether it is possible to use metformin to prevent antipsychotic-induced weight gain, and if so, how to select patients for whom such therapy can be indicated. Finally, more clinical trials are needed to evaluate the efficacy and safety of other classes of hypoglycemic and lipid-lowering drugs in patients on antipsychotics

Smart City as the Basic Construct of the Socio-economic Development of Territories

The relevance of this topic is that any self-respecting city needs a development strategy that develops on the basis of the ideal representations of the future and opportunities. All of the city strive to achieve two fundamental goals. The first is to create a high-quality living environment, which is dictated by growing competition for investment and brains. The second objective - to ensure sustainable development, i.e. the search for the integrated (economic, social, transport, energy, environmental, etc.) model, which would guarantee a dignified existence for at least several generations of citizens. Currently in addressing the challenges of economic modernization and innovative development of Russia a key role to be played by information, knowledge, the widespread use of information and communication technologies, the problems of optimum use of natural resources and ecology, as well as the formation of the social environment, designed to provide optimal conditions for the work of participants the innovation process (Shkurkin, Shestopal, Gurieva, Blaginin and Gurianov 2016). In this context, the problem of creating smart cities is today one of the most pressing. Background of the idea of “smart” city and its development: increase in workload infrastructures. Keywords: Economic development, regional development, smart city, infrastructure, resources JEL Classifications: О13, R1

Estimation of physiotherapy effectiveness in complex treatment of psoriasis

The article presents the results of placebo-controlled research of the effectiveness of magnetic infrared laser therapy (MIL-therapy) of patients with psoriasis with malabsorption syndrome. The methods of treatment (traditional method, method with the use of magnetic infrared laser therapy and placebo control) were compared. The results of study showed that the group of patients who were treated with use of magnetic infrared laser therapy had more significant positive dynamics

The problem of interaction of concepts guilt/вина and conscience/совесть in the Russian and English linguocultures

The article represents a description of different lexical means of the concepts’ guilt/вина and conscience/совесть verbalization in the English and Russian languages. The main idea of the research is to reveal the true nature of different lexical means that can motivate different meanings on the functional leve