The effects of henna leaf alcoholic extract on angiogenesis of chorioallantoic membrane and some embryonic morphological abnormalities of chick embryos

زمینه و هدف: آنژیوژنز، تشکیل مویرگ های جدید از عروق اولیه، در فرآیند های فیزیولوژیک مانند تولید مثل و بهبود زخم و پاتولوژیک مانند دیابت و تومور نقش دارد. رشد و توسعه تومور با رگ زایی ارتباط دارد. این تحقیق با هدف بررسی اثرات عصاره الکلی برگ حنا (Lawsonia inermis) بر آنژیوژنز پرده کوریوآلانتوئیک و ناهنجاری های مورفولوژیک جنین جوجه انجام شده است. روش بررسی: در این پژوهش تجربی، 40 عدد تخم مرغ نطفه دار نژاد Hy-line به طور تصادفی در 4 گروه: شاهد، شاهد آزمایشگاهی، گروه تجربی 1 و گروه تجربی 2 تقسیم شدند (10=n). روز دوم انکوباسیون در شرایط استریل، در تخم مرغ ها پنجره ایجاد گردید. روز هشتم پرده کوریوآلانتوئیک در گروه شاهد آزمایشگاهی با نرمال سالین و در گروه تجربی 1 و 2 با عصاره الکلی برگ حنا به ترتیب با دوزهای 50 و mg/kg200 تیمار گردید. روز دوازدهم از عروق ناحیه تیمار عکس برداری و تعداد و طول انشعابات عروقی اندازه گیری شد. در جنین های برداشت شده، وزن و طول سری- دمی (CR) و ناهنجاری های مورفولوژیکی مورد بررسی قرار گرفت. یافته ها: مقایسه میانگین تعداد و طول انشعابات عروقی، وزن و طول سری- دمی جنین جوجه در گروه شاهد با شاهد آزمایشگاهی اختلاف معنی دار نشان نداد (05/0>P). در گروه تجربی 2 میانگین تعداد و طول انشعابات عروقی و در گروه تجربی 1 میانگین طول انشعابات عروقی و طول سری- دمی نسبت به گروه شاهد کاهش معنی داری نشان داد (05/0>P). میانگین متغیرها در گروه های تجربی اول و دوم اختلاف معنی دار نشان نداد (05/0>P). در بررسی مورفولوژیکی جنین ها هیچگونه ناهنجاری مشاهده نشد. نتیجه گیری: نتایج این تحقیق نشانگر اثرات مهارکننده رگ زایی عصاره الکلی برگ حنا بر آنژیوژنز جنین جوجه است؛ اما بر خصوصیات مورفولوژیکی جنین جوجه تأثیر قابل توجهی ندارد

The synergic effect of glycyrrhizic acid and low frequency electromagnetic field on angiogenesis in chick chorioallantoic membrane

Objective: Much attention is paid to angiogenesis due to its mutual role in health and disease. Therefore, the effect of various chemical and physical agents on inhibition of this process has been recently studied. This study was conducted to investigate the synergic effect of glycyrrhizic acid and electromagnetic field on angiogenesis. Materials and Methods: In this experimental study, 44 Ross fertilized chicken eggs were randomly divided into four groups, one control and three experimental. Control group was kept with dimethyl sulfoxide on the eighth day, experimental group 1 treated with 200 gauss, 50 Hz electromagnetic field on the 10th day, experimental group 2 treated with 1 mg/ml glycyrrhizic acid on the eighth day, and experimental group 3 simultaneously treated with glycyrrhizic acid on the eighth day and electromagnetic field on the 10th day. On the 12th day, the images of chorioallantoic membrane samples were prepared using photostreomicroscope and the number and length of vessels were measured. Results: The mean number of vessels in the experimental groups 1 and 3 (29.31 +/- 3.60 and 27.43 +/- 4.61, respectively) was not significantly different from that in the control group (29.11 +/- 4.76) (p>0.05). The length of vessels in the experimental groups 1 and 3 (52.35 +/- 3.25 mm and 54.94 +/- 4.70 mm, respectively) decreased significantly (p<0.05) compared with the control group (61.79 +/- 6.46 mm). In experimental group 2, both length and number of vessels (54.53 +/- 5.85 mm and 23.96 +/- 3.94) decreased significantly compared with the control group (p<0.05). Conclusion: Electromagnetic field and glycyrrhizic acid separately led to inhibition of angiogenesis. However, use of electromagnetic field accompanied with glycyrrhizic acid not only did not increase but also decreased the inhibitory effect

The Synergic Effects of Atorvastatin (0/1m) and (10 m) and Extremely Low Frequency Electromagnetic Field on Angiogenesis

Background and aim: statins promote the proliferation and survival of endothelial cells. Angiogenesis is the formation of new blood vessels from pre-existing ones.It contributes to physiological and pathophysiological condition. In this research we used atorvastatin (0/1m) and (10m) with electromagnetic field on angiogenesis of chick embryo. Method: in this experiment study 98 Ross fertilized eggs were randomly divided in to 7 groups as: following:control group,1)group sham-exposed,2) group treated with atorvastatin (0/1 m), 3)group treated with atorvastatin (10m),4) group treated with electromagnetic field,5) group treated with both atorvastatin (0/1m) and electromagnetic field,6) group treated with both atorvastatin (10m) and electromagnetic field. In day2, a window was opened on eggs in sterile condition in days 8a gelatin sponge was placed on chorioalantoic membrane(CAM) and was soaked with 10l atorvastatin (0/1 m) and (10m) in groups (3,6) and groups (2 , 5) and groups (4,5,6) were placed in 50 Gauss magnetic field for 4 hours in day 10.In day 12,CAMs were examined and photographed by research photo-stereo microscope in all case. Data were analyzed statistically by t-test and ANOVA(P0/05).comparison between average number and length of vessels in control and group 2 and groups 3, 6 a significant increase and decrease was shown in the average number and length of vessels compared with control(p<0/05). Conclusion: the result of this study showed that: treated with atorvastatin 10m with electromagnetic field has an inhibitory effect on angiogenesis in CAM and was shown that electromagnetic field has an inhibitory on atorvastatin 0/1m to stimulatory effect on angiogenesis in CAM