Assessment of cd93 stem cell growth and survival on three-dimensional biodegradable pcl-gelatin scaffold

Background and purpose: Application of three-dimensional scaffolds with the ability to simulate a three-dimensional in vivo environment has opened new perspective on targeted differentiation and therapeutic use of stem cells. In this study we examined the compatibility of CD93 stem cells with biodegradable pcl- gelatin scaffold. Materials and methods: In this experimental study, three-dimensional scaffolds made of PCL -gelatin using electrospining synthesis and its molecular structure was tested by SEM electron microscopy. The scaffold surface was disinfected by UV ray. The hematopoietic CD93stem cells of those isolated previously were divided into two groups including normal cultured (plate) and culture on scaffolds (scaffold + cell). The survival and growth of the cells were measured through MTT assay and electron microscopy at 7, 14, and 28 days after culturing. Results: Electron microscopic analysis on the seventh day showed appropriate adhesion of CD93 cells on scaffold fibers and secretion of extracellular matrix. Survival rate of the cells at 7, 14, and 28 days after culturing were not significantly different between the two groups. But at the same days significant differences were observed in the Scaffold + Cell group (P< 0.05). Conclusion: This study suggests that PCL nanofiber scaffolds has high compatibility with CD93 stem cells and proximity to this scaffold lead to increased survival and growth of the cells. Further studies on the treatment of tissue damage and scarring by CD93 stem cells using this scaffold can be effective in increasing treatment efficiency. © 2016, Mazandaran University of Medical Sciences. All rights reserved

H2 Formation on the Dust Grain Surface in Divertor Plasma

Edge plasma of divertor in tokamak is characterized by different values of plasma parameters (plasmadensity and temperature), plasma composition and overcoming plasma dynamics processes. We survey amodel for theoretical study of the interaction of hydrogen with dust surface and apply our results the H2formation on dust grain surfaces in the divertor. In this model, considering both physisorbed andchemisorbed sites on the grain surface and then adatoms mobility to go from one site to other site bythermal diffusion, describes the formation of H2 on grain surfaces. We find H2 formation rate on the hightemperature dust surfaces in divertor for a range of dust and gas temperatures and densities

Age-associated changes on axonal regeneration and functional outcome after spinal cord injury in rats

This study was conducted to evaluate the association between aging and regenerative potential of spinal cord injury. Three groups of male Sprague�Dawley rats, including young (40 days), mature (5-6 months) and old (28-29 months) were spinally hemisected at the L1 level. The locomotor performance was assessed weekly for eight weeks after lesion using locomotors� rating scale developed by Basso, Bresnahan and Beattie (BBB). In the tracing study, retrograde labeled neuron was counted in the lateral vestibular nucleus for axonal regeneration. From 4-8 weeks, the functional recovery of the young and mature age rats was significantly increased in comparison to the old age group. At 8 weeks, young and mature animals achieved a plateau score of (mean ± SD), 17 ± 1.47 and 16.8 ± 0.70 respectively, and the old rats reached an average score of 13.8±1.63 (P<0.05). The mean number of labeled neurons in the vestibular nucleus in the young group (mean ± SD): 32.05 ± 1.03 increase significantly compared to the older age group 5.01 ± 1.31 (P<0.05). Current findings suggest that axonal repair and functional improvement decrease in aged animals after partial spinal cord injury. Thus, the aging process may affect the regenerative capacity of the injured central nervous system, and axonal regeneration is age dependent. © 2015 Tehran University of Medical Sciences. All rights reserved

A comparative study on the results of agonist and antagonist protocols based on serum AMH levels in patients undergoing intracytoplasmic sperm injection

Background: Serum concentrations of antimullerian hormone (AMH) correlate with ovarian response during assisted reproduction treatment (ART) cycles. Objective: This retrospective study attempted to evaluate the selection of ovarian stimulation protocols based on serum AMH levels in patients and its impact on the results of ART. Materials and Methods: Based on AMH levels, the patients with tubal factor infertility were divided in three groups of normal, low and high AMH levels. Oocyte, good embryo number and pregnancy rate in each group were analyzed. Results: Using agonist and antagonist protocols, an increase in serum AMH led to higher number of oocytes and better quality embryos. At all low, normal and high AMH levels, the agonist protocol led to a more significant increase in the number of oocytes than the antagonist protocol (p<0.05). The number of high quality embryos significantly increased by the agonist protocol than antagonist protocol in women with normal AMH levels of 1.3-2.6 ng/ml (p=0.00). Moreover, the results for the number of high quality embryos at AMH �2.6 ng/ml was in favor of the antagonist protocol (p=0.00). The results showed the lowest pregnancy rate at AMH �1.3 ng/ml. At AMH �2.6 ng/ml, there was a significant increase in pregnancy rate through the antagonist protocol (p=0.04). Conclusion: Findings of this study suggested that the ART results are predictable, taking into account the AMH levels. The protocol specific to each patient can be used given the AMH level in each individual. This is because the results of each protocol depend on individual conditions. © 2016, Research and Clinical Center for Infertitlity. All rights reserved

Remyelination of the corpus callosum by olfactory ensheathing cell in an experimental model of multiple sclerosis

Multiple Sclerosis (MS) causes loss of the myelin sheath, which leads to loss of neurons. Regeneration of myelin sheath stimulates axon regeneration and neurons� survival. In this study, olfactory ensheathing cell (OEC) transplantation is investigated to restore myelin sheath in an experimental model of MS in male mice.OECs were isolated from the olfactory mucosa of seven-day-old infant rats and cultured. Then, cells were evaluated and approved by flow cytometry by p75 and GFAP markers. A total of 32 mice (C57BL /6) were studied in four groups; 1) without any treatment (control), 2) Sham (receiving PBS), 3) MS model and 4) MS and OEC transplantation. MS was induced by adding Cuprizon in the diet of animals for six weeks. After the expiration of 20 days, histologic analysis was performed with approval of the presence of cells in the graft area and the removal of myelin and myelin regeneration with two types of luxal fast blue (LFB) staining and immunohistochemistry. The purity of the cells ensheathing the olfactory was 90. There was a significant difference in Myelin percentage of PBS and OEC recipient groups (P�0.05). MBP and PLP of the myelin sheath in the group receiving OECs were more than MS group.According to the findings, in MS model MBP and PLP of the myelin sheath is reduced. In the group receiving OECs, it was returned to a normal level significantly compared to the sham group received only PBS significant differences were observed. The OECs transplantation can improve myelin restoration. © 2015 Tehran University of Medical Sciences. All rights reserved

Accelerated Ripening of Kabkab Dates Using Sodium Chloride and Acetic Acid Solutions

ABSTRACT-In this research, the accelerated ripening of the Kabkab dates from Khalal to Tamr using NaCl and acetic acid solutions was studied. Fruits at Khalal stage were harvested and washed with distilled water before being treated with ripening inducers: 1. NaCl solutions, 2. acetic acid solutions and 3. Aqueous solution of NaCl (1%) and acetic acid. moisture, pH, colour, brix and textural firmness of the samples were monitored. Results indicated that during ripening the moisture content and colour changed significantly. The major change was observed for firmness where a maximum force for puncture test varied from about 1000 to 50 g force for all samples after 72 hours of incubation at 40 °C. Harvesting at Khalal stage followed by treating the fruits with NaCl and/or acetic acid solutions and an incubation stage at 40 °C showed to be a promising method for accelerated ripening

Preliminary Evidence of Acceptance and Commitment Therapy for Death Anxiety in Iranian Clients Diagnosed with OCD

This study investigated the effectiveness of Acceptance and Commitment Therapy (ACT) on death anxiety and obsessive-compulsive disorder (OCD) with eight adult females in Iran. The ACT protocol was conducted in 8 weekly solo sessions (45 minutes each). The results were analyzed by visual analysis method and improvement percentage. ACT resulted in decreases in death anxiety (60-80%) and obsessive-compulsive symptoms (51-60%), thereby indicating promise for ACT as a treatment for OCD and death anxiety

Anxiety as a consequence of modern dietary pattern in adults in Tehran-Iran

Food intake patterns in relation to mental health have already been revealed. To investigate the relationship between processed food consumption behavior and anxiety disorder, a cross sectional study was conducted. Overall, 1782 young adults aged 18-35 years were randomly selected using cluster sampling method from 22 districts of Tehran Iran in 2011. Diet assessment was done using a 24 hour recall questionnaire in two times with a week interval. Anxiety level was determined using the validated Speilburger test (Persian version). A proportional odds regression model was used to assess the effect of processed food consumption on anxiety variables. A significant statistical difference was found between men and women in terms of processed food consumption (p<0.001). Adjusting for age, total calorie intake, gender, body mass index, socioeconomic status, and history of sedative drug consumption as well as mental health disorders, the proportional odds regression model showed a significant relationship between increased consumption of processed foods and anxiety (OR = 4.73, 95 CI: 2.89-12.54 for state and OR = 4.91, 95 CI: 2.88-13.99 for trait). Identification, modification and adjusting incorrect food patterns in the community could be considered as valuable steps to turn down nutritional-based health difficulties. (C) 2012 Elsevier Ltd. All rights reserved

An Evaluation of the Effect of Polycaprolactone/Gelatin (Pcl/Gt) Nanofiber Scaffold on the Therapeutic Function of Hematopoietic Stem Cells

BACKGROUND AND OBJECTIVE: Hematopoietic stem cells are one of the new therapeutic options for treating skin ulcers. Recently, a new perspective has been developed to better utilize stem cells using nanofiber scaffolds. The present study was conducted to investigate the effect of polycaprolactone/gelatin (PCL/GT) nanofiber scaffold on the therapeutic function of hematopoietic stem cells. METHODS: In this experimental study, 15 male BALB / c mice were divided into three groups of five, including the control group, the group receiving stem cells in the wound site (cell group) and the group receiving cell + PCL nanofiber scaffold. PCL/GT nanofiber scaffold was prepared by electrotherapy. After hematoxylin and eosin staining, the parameters of epidermal repair and hair follicle formation in the wound site were evaluated by fluorescence microscope and Image J and SPSS programs. FINDINGS: On day 28 after transplantation, the highest and lowest epidermal thicknesses were observed in the cell + scaffold group and control group, which were 10.5±0.3 and 27.3±0.9 μm, respectively, which was significant (p<0.05). Moreover, the highest and lowest number of hair follicles were observed in the cell + scaffold group and control group, respectively; 5.2±0.2 and 4.2±0.3. The difference between the two groups was significant (p<0.01) CONCLUSION: Polycaprolactone / gelatin (PCL/GT) nanofiber scaffold significantly increases the therapeutic function of hematopoietic stem cells in the wound site

Transplantation of olfactory mucosa improve functional recovery and axonal regeneration following sciatic nerve repair in rats

Background: Olfactory ensheathing glia (OEG) has been shown to have a neuroprotective effect after being transplanted in rats with spinal cord injury. This study was conducted to determine the possible beneficial results of olfactory mucosa transplantation (OMT) which is a source of OEG on functional recovery and axonal regeneration after transection of the sciatic nerve. Methods: In this study, 36 adult female Sprague-Dawley rats were used. The sciatic nerve was transected in 24 rats and immediately repaired by sciatic-sciatic anastomosis, and randomly divided equally into two groups. The experimental group received the OMT at the transected site and the control group received the respiratory mucosa transplant. In another twelve rats as sham-operated animals, the sciatic nerve was exposed but no transection was made. DiI retrograde tracing was injected in the gastrocnemius muscle two months after surgery to allow visualization of the extent of axonal regeneration. Functional recovery was also assessed at 15, 30, 45 and 60 days after surgery using walking track analysis and sciatic function index (SFI) calculations. Results: The total number of DiI labeled motorneurones in the ventral horn (L4-L6) and the SFI scores were significantly higher in the group of rats that received olfactory mucosa rather than respiratory mucosa. Conclusions: The outcome indicates that olfactory mucosa is a useful treatment to improve nerve regeneration in mammals with peripheral nerve injury