Designing a Music Listening Program for the Junior High School General Music Class of Columbia View Intermediate School

Faced with the need for providing a listening program without the assistance of an available curriculum guide in junior high school music for the Reynolds School District No. 7, it was the purpose of this writer to construct a design for a program of instruction in music listening. The suggested design is based upon an examination of literature related to listening and is illustrated by sample materials provided in the appendices

Cotton Price Policy and New Cereal Technology in the Malian Cotton Zone

During the last decade, cotton production and area have been declining as a result of depleting soil nutrients and low cotton prices in the cotton zone of Mali. This paper shows that the Malian government’s 2011 policy to increase the farm gate cotton price as a response to world cotton price increase enhances farm income but has less impact on cotton than on maize production. A complementary policy of introducing new sorghum technologies would have an equal impact on farmers’ incomes in the cotton zone of Mali.Cotton prices, improved sorghum technology, discrete stochastic programming, Mali, Agricultural and Food Policy, Farm Management, International Development, Production Economics, Risk and Uncertainty,

Developmentally Programmed Rearrangement of T Cell Receptor Vγ Genes Is Controlled by Sequences Immediately Upstream of the Vγ Genes

AbstractDistinct subsets of γδ T cells expressing different Vγ and Vδ chains arise in ordered waves during thymic development. In the murine Jγ1–Cγ1 cluster, the Vγ3 gene segment is utilized earliest in fetal thymic development, in progenitors of dendritic epidermal T cells (DECs). The Vγ2 gene segment predominates in the late fetal stages and beyond, in cells destined for the secondary lymphoid organs. Using transgenic TCRγ recombination substrates, we demonstrate that this restricted Vγ gene usage is determined by developmentally targeted gene rearrangement. We show that sequences immediately upstream of the Vγ2 and Vγ3 genes direct the rearrangement pattern in adult thymocytes. Thus, the choice of Vγ gene for recombination is coordinated with distinct differentiation programs in γδ subsets

The use of TOMS-modified VAS data for large-scale NWP

Collocation statistics obtained by comparing VISSR Atmospheric Sounder (VAS) temperature soundings with those from nearby rawinsondes indicate good agreement. However, the VAS soundings exhibited a substantial cold bias in the middle and upper troposphere. The error makes promising the use of the Total Ozone Mapping Spectrometer (TOMS) to obtain an independent estimate of tropopause pressure for use in the retrieval program. Good agreement is found between TOMS data and tropopause pressure. A quantitative assessment for the correlation of tropopause pressure, obtained from TOMS by regression and from rawinsondes over Europe, is reported

A Novel Element Upstream of the Vγ2 Gene in the Murine T Cell Receptor γ Locus Cooperates with the 3′ Enhancer to Act as a Locus Control Region

Transgenic expression constructs were employed to identify a cis-acting transcription element in the T cell receptor (TCR)-γ locus, called HsA, between the Vγ5 and Vγ2 genes. In constructs lacking the previously defined enhancer (3′ECγ1), HsA supports transcription in mature but not immature T cells in a largely position-independent fashion. 3′ECγ1, without HsA, supports transcription in immature and mature T cells but is subject to severe position effects. Together, the two elements support expression in immature and mature T cells in a copy number–dependent, position-independent fashion. Furthermore, HsA was necessary for consistent rearrangement of transgenic recombination substrates. These data suggest that HsA provides chromatin-opening activity and, together with 3′ECγ1, constitutes a T cell–specific locus control region for the TCR-γ locus

Antigenic Protein In Microgravity-Grown Human Mixed Mullerian Tumor (LN1) Cells Preserved In RNA Stabilizing Agent

Cells treated with RNAlater(TradeMark) have previously been shown to contain antigenic proteins that can be visualized using Western blot analysis. These proteins seem to be stable for several months when stored in RNA stabilizer at 4 C. Antigenic protein can be recovered from cells that have been processed using an Ambion RNAqueous(Registered TradeMark) kit to remove RNA. In this set of experiments, human mixed Mullerian tumor (LN1) cells grown on the International Space Station during Expedition 3 were examined for antigenic stability after removal of RNA. The cells were stored for three months in RNAlater(TradeMark) and RNA was extracted. The RNA filtrate Containing the protein was precipitated, washed, and suspended in buffer containing sodium dodecyl sulfate (SDS). Samples containing equal concentrations of protein were loaded onto SDS-polyacrylamide gels. Proteins were separated by electrophoresis and transferred by Western blot to polyvinylidene fluoride (PVDF) membrane. The Western blots were stained with an enhanced chemiluminescent ECL(Registered TradeMark)Plus detection kit (Amersham) and scanned using a Storm 840 gel image analyzer (Amersham, Molecular Dynamics). ImageQuant(Registered TradeMark)a software was used to quantify the densities of the protein bands. The ground control and flight LN1 cell samples showed a similar staining pattern over time with antibodies to vimentin, glyceraldehyde-3-phosphate dehydrogenase, and epithelial membrane antigens

One Banana

This book was completed for Jan Baker\u27s artists\u27 book class.https://digitalcommons.risd.edu/specialcollections_bookmark_culture/1027/thumbnail.jp

A risk stratification tool to assess commecial influences on continuing medical education

Introduction: Heightened concerns about industry influence on continuing medical education (CME) have prompted tighter controls on the management of commercial funding and conflict of interest. As a result, CME providers must closely monitor their activities and intervene if bias or noncompliance with accreditation standards is likely. Potential for industry influence can be difficult to assess at a stage in the planning process when mitigation strategies can assure balance and content validity. Few tools exist to aid providers in this regard. Methods: A 12-item instrument was designed to assess risk for commercial influence on CME. To determine reliability and validity, a cohort of experienced CME professionals applied the tool to standardized cases representing CME activities in the early stages of planning. Results were compared with the experts\u27 assignment of the same cases to one of four risk categories. A survey of study participants was conducted to ascertain usefulness and potential applications of the tool. Results: Analysis demonstrated strong intraclass correlation across cases (0.90), interrater reliability (94%), and correlation between assessment of risk with and without the tool (Spearman coefficient, 0.93, p \u3c 0.01; weighted kappa, 0.59). Participants found the tool easy to use and of potential benefit to their CME office. Discussion: The Consortium for Academic Continuing Medical Education (CACME) risk stratification tool can help CME providers identify activities that must be closely monitored for potential industry influence, remain aware of factors that place programming at risk for noncompliance with accreditation standards, and substantiate the allocation of resources by the CME office

Spatial re-organization of myogenic regulatory sequences temporally controls gene expression

During skeletal muscle differentiation, the activation of some tissue-specific genes occurs immediately while others are delayed. The molecular basis controlling temporal gene regulation is poorly understood. We show that the regulatory sequences, but not other regions of genes expressed at late times of myogenesis, are in close physical proximity in differentiating embryonic tissue and in differentiating culture cells, despite these genes being located on different chromosomes. Formation of these inter-chromosomal interactions requires the lineage-determinant MyoD and functional Brg1, the ATPase subunit of SWI/SNF chromatin remodeling enzymes. Ectopic expression of myogenin and a specific Mef2 isoform induced myogenic differentiation without activating endogenous MyoD expression. Under these conditions, the regulatory sequences of late gene loci were not in close proximity, and these genes were prematurely activated. The data indicate that the spatial organization of late genes contributes to temporal regulation of myogenic transcription by restricting late gene expression during the early stages of myogenesis. The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research