The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations

<p>Abstract</p> <p>Background</p> <p>Single-stranded RNA viruses, infectious to the European honeybee, <it>Apis mellifera </it>L. are known to reside at low levels in colonies, with typically no apparent signs of infection observed in the honeybees. Reverse transcription-PCR (RT-PCR) of regions of the RNA-dependent RNA polymerase (RdRp) is often used to diagnose their presence in apiaries and also to classify the type of virus detected.</p> <p>Results</p> <p>Analysis of RdRp conserved domains was undertaken on members of the newly defined order, the Picornavirales; focusing in particular on the amino acid residues and motifs known to be conserved. Consensus sequences were compiled using partial and complete honeybee virus sequences published to date. Certain members within the iflaviruses, deformed wing virus (DWV), Kakugo virus (KV) and <it>Varroa destructor </it>virus (VDV); and the dicistroviruses, acute bee paralysis virus (ABPV), Israeli paralysis virus (IAPV) and Kashmir bee virus (KBV), shared greater than 98% and 92% homology across the RdRp conserved domains, respectively.</p> <p>Conclusion</p> <p>RdRp was validated as a suitable taxonomic marker for the assignment of members of the order Picornavirales, with the potential for use independent of other genetic or phenotypic markers. Despite the current use of the RdRp as a genetic marker for the detection of specific honeybee viruses, we provide overwhelming evidence that care should be taken with the primer set design. We demonstrated that DWV, VDV and KV, or ABPV, IAPV and KBV, respectively are all recent descendents or variants of each other, meaning caution should be applied when assigning presence or absence to any of these viruses when using current RdRp primer sets. Moreover, it is more likely that some primer sets (regardless of what gene is used) are too specific and thus are underestimating the diversity of honeybee viruses.</p

Implementing Behavior Analysis and Intervention for Individuals with Cognitive Impairments in Skilled Nursing Facilities: Summary of Results

Summary of Purpose The purpose of the project was to provide behavioral consultation and services to aging persons with cognitive impairment at skilled nursing facilities in Michigan. The goal was to use empirically supported non-pharmacological approaches to reduce behavioral and psychological symptoms of dementia (BPSD; wandering, agitation, disruptive vocalizations, etc.) and help slow down or remediate lost skills, reduce the use of medication to manage BPSD, to improve staff knowledge and abilities, and to develop modules that can be adopted and used by other skilled nursing facilities.The project was led by Dr. Janet Hahn, a social gerontologist with extensive experience studying nursing home culture change and the quality of long-term care services. The intervention project team consisted of doctoral, masters and undergraduate level behavior analysts with advanced training in working with aging populations, under the direction of Dr. Jonathan Baker (doctoral level board certified behavior analyst and behavioral gerontologist). The project was funded by the Civil Money Penalties fund of the Michigan Department of Health and Human Services from May 2016 and to April 2019. The project was conducted with oversight by the Western Michigan University Human Subjects Institutional Review Board, under approved protocol HSIRB Project Number 16-09-07, titled Implementing Behavior Analysis and Intervention for Individuals with Cognitive Impairment in Skilled Nursing Facilities

Paleoclimate of the subtropical Andes during the latest Miocene, Lauca T Basin, Chile

Uplift of the Andean Cordillera during the Miocene and Pliocene produced large-scale changes in regional atmospheric circulation that impacted local ecosystems. The Lauca Basin (northern Chilean Altiplano) contains variably fluvial and lacustrine sedimentary sequences spanning the interval from 8.7 to 2.3 Ma. Field samples were collected from paleo-lacustrine sediments in the basin. Sediments were dated using detrital zircon geochronology on volcanic tuffs, yielding an age range between ~5.57 and 5.44 Ma. These new age constraints provided an opportunity to evaluate changes in the Lauca Basin ecosystem across this dynamic Miocene-Pliocene transition. We employed multiple proxies (lithofacies analysis, diatoms, pollen, and oxygen stable isotopes of authigenic carbonates) to interpret ancient lacustrine and terrestrial paleoenvironments. Alternations among mudstone, carbonate, and evaporitic facies indicate lake-level variability through time. The diatom assemblage is characterized by meso- to hypersaline and alkaline-tolerant taxa typical of shallow lakes. The δ18O values ranged from −8.96 to −2.22‰ indicating fluctuations in water balance. Pollen taxa in the outcrop are typical of a transitional stage between seasonal cloud forest and open grassland. Together, these proxies indicate that the Lauca paleolake sediments were deposited under a wetter-than-modern climate with high temporal variability. Our results refine previous studies in the Lauca Basin and are consistent with other regional studies suggesting that the South American summer monsoon at the Miocene-Pliocene transition was more intense than it is at present

UV Spectropolarimetry of Narrow-line Radio Galaxies

We present the results of UV spectropolarimetry (2000 - 3000A) and far-UV spectroscopy (1500 - 2000A) of two low-redshift narrow-line radio galaxies (NLRGs) taken with the Faint Object Spectrograph onboard the Hubble Space Telescope (HST). Spectropolarimetry of several NLRGs has shown that, by the presence of broad permitted lines in polarized flux spectrum, they have hidden quasars seen through scattered light. Imaging polarimetry has shown that NLRGs including our targets often have large scattering regions of a few kpc to >~10 kpc scale. This has posed a problem about the nature of the scatterers in these radio galaxies. Their polarized continuum has the spectral index similar to or no bluer than that of quasars, which favors electrons as the dominant scattering particles. The large scattering region size, however, favors dust scattering, because of its higher scattering efficiency compared to electrons. In this paper, we investigate the polarized flux spectrum over a wide wavelength range, combining our UV data with previous optical/infrared polarimetry data. We infer that the scattering would be often caused by opaque dust clouds in the NLRGs and this would be a part of the reason for the apparently grey scattering. In the high-redshift radio galaxies, these opaque clouds could be the proto-galactic subunits inferred to be seen in the HST images. However, we still cannot rule out the possibility of electron scattering, which could imply the existence of a large gas mass surrounding these radio galaxies.Comment: 25 pages, 21 figures. To appear in Ap

The 3-D O(4) universality class and the phase transition in two-flavor QCD

We determine the critical equation of state of the three-dimensional O(4) universality class. We first consider the small-field expansion of the effective potential (Helmholtz free energy). Then, we apply a systematic approximation scheme based on polynomial parametric representations that are valid in the whole critical regime, satisfy the correct analytic properties (Griffiths' analyticity), take into account the Goldstone singularities at the coexistence curve, and match the small-field expansion of the effective potential. From the approximate representations of the equation of state, we obtain estimates of several universal amplitude ratios. The three-dimensional O(4) universality class is expected to describe the finite-temperature chiral transition of quantum chromodynamics with two light flavors. Within this picture, the O(4) critical equation of state relates the reduced temperature, the quark masses, and the condensates around T_c in the limit of vanishing quark masses.Comment: 19 pages, 5 fig

Coherent Bayesian analysis of inspiral signals

We present in this paper a Bayesian parameter estimation method for the analysis of interferometric gravitational wave observations of an inspiral of binary compact objects using data recorded simultaneously by a network of several interferometers at different sites. We consider neutron star or black hole inspirals that are modeled to 3.5 post-Newtonian (PN) order in phase and 2.5 PN in amplitude. Inference is facilitated using Markov chain Monte Carlo methods that are adapted in order to efficiently explore the particular parameter space. Examples are shown to illustrate how and what information about the different parameters can be derived from the data. This study uses simulated signals and data with noise characteristics that are assumed to be defined by the LIGO and Virgo detectors operating at their design sensitivities. Nine parameters are estimated, including those associated with the binary system, plus its location on the sky. We explain how this technique will be part of a detection pipeline for binary systems of compact objects with masses up to 20 \sunmass, including cases where the ratio of the individual masses can be extreme.Comment: Accepted for publication in Classical and Quantum Gravity, Special issue for GWDAW-1

Critical structure factors of bilinear fields in O(N)-vector models

We compute the two-point correlation functions of general quadratic operators in the high-temperature phase of the three-dimensional O(N) vector model by using field-theoretical methods. In particular, we study the small- and large-momentum behavior of the corresponding scaling functions, and give general interpolation formulae based on a dispersive approach. Moreover, we determine the crossover exponent $\phi_T$ associated with the traceless tensorial quadratic field, by computing and analyzing its six-loop perturbative expansion in fixed dimension. We find: $\phi_T=1.184(12)$, $\phi_T=1.271(21)$, and $\phi_T=1.40(4)$ for $N=2,3,5$ respectively.Comment: 27 page

A Review of Volatile Organic Compound Contamination in Post-Industrial Urban Centers: Reproductive Health Implications Using a Detroit Lens

Volatile organic compounds (VOCs) are a group of aromatic or chlorinated organic chemicals commonly found in manufactured products that have high vapor pressure, and thus vaporize readily at room temperature. While airshed VOCs are well studied and have provided insights into public health issues, we suggest that belowground VOCs and the related vapor intrusion process could be equally or even more relevant to public health. The persistence, movement, remediation, and human health implications of subsurface VOCs in urban landscapes remain relatively understudied despite evidence of widespread contamination. This review explores the state of the science of subsurface movement and remediation of VOCs through groundwater and soils, the linkages between these poorly understood contaminant exposure pathways and health outcomes based on research in various animal models, and describes the role of these contaminants in human health, focusing on birth outcomes, notably low birth weight and preterm birth. Finally, this review provides recommendations for future research to address knowledge gaps that are essential for not only tackling health disparities and environmental injustice in post-industrial cities, but also protecting and preserving critical freshwater resources