Fibulin-3 is necessary to prevent cardiac rupture following myocardial infarction

Despite the high prevalence of heart failure in the western world, there are few effective treatments. Fibulin-3 is a protein involved in extracellular matrix (ECM) structural integrity, however its role in the heart is unknown. We have demonstrated, using single cell RNA-seq, that fibulin-3 was highly expressed in quiescent murine cardiac fibroblasts, with expression highest prior to injury and late post-infarct (from ~ day-28 to week-8). In humans, fibulin-3 was upregulated in left ventricular tissue and plasma of heart failure patients. Fibulin-3 knockout (Efemp1−/−) and wildtype mice were subjected to experimental myocardial infarction. Fibulin-3 deletion resulted in significantly higher rate of cardiac rupture days 3–6 post-infarct, indicating a weak and poorly formed scar, with severe ventricular remodelling in surviving mice at day-28 post-infarct. Fibulin-3 knockout mice demonstrated less collagen deposition at day-3 post-infarct, with abnormal collagen fibre-alignment. RNA-seq on day-3 infarct tissue revealed upregulation of ECM degradation and inflammatory genes, but downregulation of ECM assembly/structure/organisation genes in fibulin-3 knockout mice. GSEA pathway analysis showed enrichment of inflammatory pathways and a depletion of ECM organisation pathways. Fibulin-3 originates from cardiac fibroblasts, is upregulated in human heart failure, and is necessary for correct ECM organisation/structural integrity of fibrotic tissue to prevent cardiac rupture post-infarct

Development and characterization of a preclinical model of breast cancer lung micrometastatic to macrometastatic progression.

Most cancer patients die with metastatic disease, thus, good models that recapitulate the natural process of metastasis including a dormancy period with micrometastatic cells would be beneficial in developing treatment strategies. Herein we report a model of natural metastasis that balances time to complete experiments with a reasonable dormancy period, which can be used to better study metastatic progression. The basis for the model is a 4T1 triple negative syngeneic breast cancer model without resection of the primary tumor. A cell titration from 500 to 15,000 GFP tagged 4T1 cells implanted into fat pad number four of immune proficient eight week female BALB/cJ mice optimized speed of the model while possessing metastatic processes including dormancy and beginning of reactivation. The frequency of primary tumors was less than 50% in animals implanted with 500-1500 cells. Although implantation with over 10,000 cells resulted in 100% primary tumor development, the tumors and macrometastases formed were highly aggressive, lacked dormancy, and offered no opportunity for treatment. Implantation of 7,500 cells resulted in >90% tumor take by 10 days; in 30-60 micrometastases in the lung (with many animals also having 2-30 brain micrometastases) two weeks post-implantation, with the first small macrometastases present at five weeks; many animals displaying macrometastases at five weeks and animals becoming moribund by six weeks post-implantation. Using the optimum of 7,500 cells the efficacy of a chemotherapeutic agent for breast cancer, doxorubicin, given at its maximal tolerated dose (MTD; 1 mg/kg weekly) was tested for an effect on metastasis. Doxorubicin treatment significantly reduced primary tumor growth and lung micrometastases but the number of macrometastases at experiment end was not significantly affected. This model should prove useful for development of drugs to target metastasis and to study the biology of metastasis

Summary of optimal cell number determination in the development of the metastatic mouse model.

<p>(<b>A</b>) Chart indicating the benefits of optimizing cell number for primary tumor take and metastatic progression in the 4T1 breast cancer model. (<b>B</b>) Percent tumor take in immune-proficient BALB/cJ mice implanted with 4T1-Luc2GFP cells into the number four mammary fat pad. Data are actual percent tumor take based on number of animals indicated above each bar from no fewer than nine independent experiments. Micrometastasis (<b>C</b>) and macrometastasis (<b>D</b>) in lung six weeks after implantation. Data are mean ± <i>SEM</i> from two independent experiments with <i>n</i> = 5 for experiment one, <i>n</i> = 10 for experiment two.</p

Efficacy of traditional chemotherapeutic agent in the 4T1 Luc2GPF mouse model.

<p>BALB/cJ mice implanted with 7,500 4T1-Luc2GFP cells were treated with the maximal tolerated dose (MTD) of doxorubicin, the dose shown not to result in any gross toxicity to the animal but shown to result in a small but insignificant loss in weight over the course of the experiment; or with carrier (5% Pharmasolve and 5% Solutol HS in saline), or with injectable saline as control once a week after visible primary tumor formation, for five weeks. (<b>A</b>) Flowchart indicating timing of cell implantation, doxorubicin treatments, and experimental determinations. (<b>B</b>) Tumors volume (mm³) over time measured by calipers. (<b>C</b>) End weight of tumors (mg), (<b>D</b>) Lung micrometastases. (<b>E</b>) Lung large micrometastases. (<b>F</b>) Lung macrometastases. Data representative of two independent experiments <i>n</i> = 15 mice for first experiment, and <i>n</i> = 10 mice for second experiment. Tumor volumes were calculated using the ellipsoidal method, volume (mm³) = 0.52 × length × width². Data are mean ± <i>SEM</i> and a two way ANOVA/Tukey post-test was performed (*p<0.05 and **p<0.01).</p

Development and quantification of lung and brain metastases: Timecourse.

<p>Micrometastases examination in lungs and brain of BALB/cJ mice implanted with 7,500 4T1-Luc2GFP cells into mammary fat pad. Lungs and brain excised, examined for metastases, degree of vascularity, and imaged at weeks two-five. (<b>A</b>) Lung micrometastases. (<b>B</b>) Lung large micrometastases. (<b>C</b>) Lung macrometastases. (<b>D</b>) Brain micrometastases. (<b>E</b>) Brain large micrometastases. All data are mean ± <i>SEM</i> from two independent experiments with 5–8 mice for experiment one and 7–11 mice for experiment two.</p

Review of literature on the 4T1 model being used for metastasis evaluation.

<p>Review of literature on the 4T1 model being used for metastasis evaluation.</p

Development of lung metastasis in the 4T1 Luc2GFP mouse model: Timecourse.

<p>(<b>A</b>) Fluorescent image of a fully vascularized primary tumor removed five weeks after 7,500 4T1-Luc2GFP cells (green in all images) implanted into BALB/cJ mammary fat pad. Vasculature (red) in primary tumor (<b>A</b>) and five weeks lung metastasis (<b>F</b>) are indicated by arrows and was achieved by retro-orbital injection of tetramethylrhodamine labeled 2×10⁶ MW dextran. At weeks one and two (<b>B</b> and <b>C</b>) micrometastases, which are defined as single-to small clusters of cells, are present in lungs. Large micrometastases lacking blood vessels are present in lungs by weeks three and four (<b>D</b> and <b>E</b>), and by week five (<b>F</b>) macrometastases containing visible blood vessels are present in the lungs.</p

Growth of 4T1-Luc2GFP primary tumors: Timecourse.

<p>Primary tumor growth was recorded over a five week period from BALB/cJ mice implanted with 7,500 4T1-Luc2GFP cells into the mammary fat pad. (<b>A</b>) Tumor volume (mm³). (<b>B</b>) Tumor weight (mg). (<b>C</b>) Animal weights (g). Tumor volumes were calculated using the ellipsoidal method, volume (mm³) = 0.52×length × width². All data are mean ± <i>SEM</i> from two independent experiments with 5–8 mice for experiment one and 7–11 mice for experiment two and are fitted to a Gomperzian growth curve by Prism 6.0 software.</p

Fibulin-3 is necessary to prevent cardiac rupture following myocardial infarction.

Despite the high prevalence of heart failure in the western world, there are few effective treatments. Fibulin-3 is a protein involved in extracellular matrix (ECM) structural integrity, however its role in the heart is unknown. We have demonstrated, using single cell RNA-seq, that fibulin-3 was highly expressed in quiescent murine cardiac fibroblasts, with expression highest prior to injury and late post-infarct (from ~ day-28 to week-8). In humans, fibulin-3 was upregulated in left ventricular tissue and plasma of heart failure patients. Fibulin-3 knockout (Efemp1-/-) and wildtype mice were subjected to experimental myocardial infarction. Fibulin-3 deletion resulted in significantly higher rate of cardiac rupture days 3-6 post-infarct, indicating a weak and poorly formed scar, with severe ventricular remodelling in surviving mice at day-28 post-infarct. Fibulin-3 knockout mice demonstrated less collagen deposition at day-3 post-infarct, with abnormal collagen fibre-alignment. RNA-seq on day-3 infarct tissue revealed upregulation of ECM degradation and inflammatory genes, but downregulation of ECM assembly/structure/organisation genes in fibulin-3 knockout mice. GSEA pathway analysis showed enrichment of inflammatory pathways and a depletion of ECM organisation pathways. Fibulin-3 originates from cardiac fibroblasts, is upregulated in human heart failure, and is necessary for correct ECM organisation/structural integrity of fibrotic tissue to prevent cardiac rupture post-infarct

People from refugee and asylum-seeking backgrounds: An open access annotated bibliography (3rd edition)

The third edition of this open access, annotated bibliography has been curated by a collective of scholars who share an interest in the impacts of forced migration on people from refugee, asylum seeking and Culturally and Linguistically Diverse (CALD) migrant backgrounds. These resources are intended to be shared with the international community of researchers, students, educators and practitioners who work with, or are interested in, forced migration, education, employment and resettlement