32 research outputs found
Visualization of Bonghan Microcells by Electron and Atomic Force Microscopy
*Corresponding author. Biomedical Physics Laboratory, Department of Physics, Seoul National University, Seoul 151-747, Korea.
E-mail: [email protected]: The origin of adult stem cells remains an open question. If they derive
from embryos, it is difficult to determine the mechanism which interrupts their
differentiation during tissue formation. In the 1960s, the Bonghan microcell was
suggested as one possible, yet to be described, route of stem cell production, such
that they have the potential to proliferate to produce normal cells.
Materials and Methods: In this study, Bonghan microcells were isolated from
Bonghan tissues on rat organ surfaces, and their detailed morphology examined by
electron and atomic force microscopy.
Results: The ultrastructure observed distinguished them from apoptotic bodies and
other microorganisms, and their unique, possible proliferation feature, as protruding
threads, was imaged by atomic force microscopy
Visualization of Bonghan Microcells by Electron and Atomic Force Microscopy
*Corresponding author. Biomedical Physics Laboratory, Department of Physics, Seoul National University, Seoul 151-747, Korea.
E-mail: [email protected]: The origin of adult stem cells remains an open question. If they derive
from embryos, it is difficult to determine the mechanism which interrupts their
differentiation during tissue formation. In the 1960s, the Bonghan microcell was
suggested as one possible, yet to be described, route of stem cell production, such
that they have the potential to proliferate to produce normal cells.
Materials and Methods: In this study, Bonghan microcells were isolated from
Bonghan tissues on rat organ surfaces, and their detailed morphology examined by
electron and atomic force microscopy.
Results: The ultrastructure observed distinguished them from apoptotic bodies and
other microorganisms, and their unique, possible proliferation feature, as protruding
threads, was imaged by atomic force microscopy
Measurement of Reactive Hydroxyl Radical Species Inside the Biosolutions During Non-thermal Atmospheric Pressure Plasma Jet Bombardment onto the Solution
Physicochemical factors that affect electroporation of lung cancer and normal cell lines
Electroporation is used for cancer therapy to efficiently destroy cancer tissues by transferring anticancer drugs into cancer cells or by irreversible tumor ablation without resealing pores. There is growing interest in the electroporation method for the treatment of lung cancer, which has the highest mortality rate among cancers. Improving the cancer cell selectivity has the potential to expand its use. However, the factors that influence the cell selectivity of electroporation are debatable. We aimed to identify the important factors that influence the efficiency of electroporation in lung cells. The electropermeabilization of lung cancer cells (H460, A549, and HCC1588) and normal lung cells (MRCS, WI26 and L132) was evaluated by the transfer of fluorescence dyes. We found that membrane permeabilization increased as cell size, membrane stiffness, resting transmembrane potential, and lipid cholesterol ratio increased. Among them, lipid composition was found to be the most relevant factor in the electroporation of lung cells. Our results provide insight into the differences between lung cancer cells and normal lung cells and provide a basis for enhancing the sensitivity of lung cancers cells to electroporation. (C) 2019 Elsevier Inc. All rights reserved.N
Measurement of Reactive Hydroxyl Radical Species Inside the Biosolutions During Non-thermal Atmospheric Pressure Plasma Jet Bombardment onto the Solution
UV Absorption Spectroscopy for the Diffusion of Plasma-Generated Reactive Species through a Skin Model
Skin applications of non-thermal atmospheric pressure plasma (NTAPP) have been at-tracting attention from medical and cosmetic aspects. The reactive species generated from plasma sources have been known to play important roles in the skin. For proper applications, it is essential to know how they diffuse into the skin. In this study, the penetration of active species from NTAPP through a skin model was analyzed by UV absorption spectroscopy. The diffusions of hydrogen peroxide, nitrite, and nitrate were quantified through curve fitting. We utilized an agarose gel to mimic epidermis and dermis layers, and we used a lipid film or a pig skin sample to mimic the stratum corneum (SC). The diffusion characteristics of reactive species through this skin model and the limitations of this method were discusse