Effect of probiotics and acidifiers on feed intake, egg mass, production performance, and egg yolk chemical composition in late-laying quails

Background and Aim: Probiotics can be used as an alternative to antibiotic growth promoters because antibiotics are prohibited worldwide. This study investigated the potential combination of probiotics and acidifiers to improve feed intake, productive performance, egg mass, and egg yolk chemical composition of late-laying quail for the health of humans who consume quail products. Materials and Methods: One hundred laying quails were divided into 4 × 5 treatments, with each group consisting of five replications. The adaptation period was 2 weeks, and the treatment was continued for 4 weeks. Probiotics and acidifiers were added to drinking water and incorporated into the diet. Feed and water were provided ad libitum. Treatment duration (1 week, 2 weeks, 3 weeks, and 4 weeks) and additional feed treatment (control, probiotic 2% + 0.5% acidifier, probiotic 2% + 1% acidifier, probiotic 4% + 0.5% acidifier, and probiotic 4% + 1% acidifier, respectively). Results: Significant differences (p < 0.05) were observed in feed intake, quail day production, feed efficiency, egg mass in laying quails, and the chemical composition of egg yolk with probiotics and acidifiers in late-laying quails. Conclusion: The combination of probiotics and acidifiers can improve feed intake, production performance, egg mass, and egg yolk chemical composition in late-laying quails

In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

Aim: This study was conducted to determine the anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark against Haemonchus contortus. Materials and Methods: Ethanol extract of bark (E.E.B.) waste and aqueous extract of bark (A.E.B.) waste of P. falcataria (at concentrations 0, 0.2, 0.4, 0.8, 1, 2.5, and 5%) and albendazole (2 mg/ml) as the positive control were placed in separate Petri dishes (50 mm). Twenty H. contortus worms were placed in Petri dishes and incubated at 37°C for 0.5, 1, 2, 3, 4, 5, 6, and 12 h. Mortality of each worm was ensured by pressing the body of the worm with a pair of tweezers and keeping it in lukewarm water for 5 min before declaring it dead. Mortality is defined as amount of death individuals and time of mortality of each worm was recorded. The parasites were then observed using scanning electron microscopy (SEM) at an accelerating voltage of 15 Kv. Statistical analysis was performed using SPSS 21.0 software, two-way ANOVA followed by Tukey's test to detect significant differences (p<0.05). The result was expressed as the mean ± standard deviation. Results: The E.E.B. and A.E.B. of P. falcataria contained active compounds, such as tannin, alkaloid, flavonoid, saponin, steroid, and triterpenoid. E.E.B. had a higher content of phenol, while A.E.B. had a higher content of flavonoid. In this study, P. falcataria showed a significant effect (p=0.00) on H. contortus in vitro. E.E.B. (0.8%) was able to exterminate H. contortus completely after 6 h, more effective than A.E.B. (5%) while the positive control requires (2 mg/ml) after 2 h. SEM analysis of the worm treated with E.E.B. and A.E.B. showed damaged cuticle structure. Conclusion: The aqueous and ethanol extracts of P. falcataria bark waste demonstrated anthelmintic activity against H. contortus

Prevalence of gastrointestinal worms in Wonosobo and thin-tailed sheep on the slope of Mount Sumbing, Central Java, Indonesia

Aim: This study was conducted to determine the prevalence of gastrointestinal (GI) worms in Wonosobo and thin-tailed sheep from the slope of Mount Sumbing. Materials and Methods: Fecal samples (n=305) were collected directly from the rectum of Wonosobo and thin-tailed sheep during the dry and rainy seasons in Wonosobo Regency, Central Java Province, Indonesia. The presence of GI helminth eggs in the fecal samples was assessed using the modified McMaster egg counting technique. The identification of the eggs or oocysts was done on the basis of their morphology and size. Results: The highest prevalence of GI worms was observed in male thin-tailed sheep (76.47%) during the rainy season, whereas the lowest prevalence was observed in female Wonosobo sheep (47.36%) during the dry season. The types of GI nematodes observed in these two types of sheep were Haemonchus contortus, Ostertagia spp., Trichostrongylus spp., Bunostomum spp., Trichuris spp., and Moniezia spp. The GI worms with the highest prevalence were of Haemonchus spp. and were observed in male thin-tailed sheep. The prevalences of the two types of sheep assessed at an altitude of 1150 m above sea level were higher than those observed at the altitude of 1586 m. The prevalence of clinical and sub-clinical parasites infestation in Wonosobo and thin-tailed sheep in Kwadungan village was significantly different (p<0.05). Conclusion: This study showed that two types of local sheep on the slope of Mount Sumbing are infected with various GI worms during the dry and rainy seasons. The highest prevalence of GI worms was found in thin-tailed sheep at an altitude of 1150 m above sea level during the rainy season, with H. contortus being the most prevalent GI parasites

Alternative Strategies of Plant Metabolite Secondary “Tannin” for Methane Emissions Reduction on Ruminant Livestock a Reviews of the Last 5 Years Literature

An increasing global population will link the increasing livestock sector contribution to meet food security. Recently, livestock production has encountered great challenges related to excessive methane emissions that have a negative impact on the environment. It requires special attention to the loss of feed energy from the methane gas formation process. Public fretfulness agreed that the gas produced by ruminants is a big factor in the effects of global warming. Alternative the decrease in methane production from ruminants is by utilizing secondary metabolite compounds in plants. These studies are interesting to be continued and explored in an effort to reduce methane production through in vitro and in vivo, because it is proven that there are many types of biological or agro-industrial waste in the world different contents, structures and benefits. This review of the last 5 years related to the utilization of tannin active compounds showed the effect on the reduction of methane production. Condensed tannin (CT) and hydrolyzed tannin (HT) types both play an important role in reducing methane, but CT is widely studied because of its presence which is more commonly found. The concept of tannin utilization still presents its own challenges to focus on the dose of administration, the structure of the tannin itself, the substitution of other ingredients and also includes the types of animals given treatment

Anthelmintic activity of Carica pubescens aqueous seed extract and its effects on rumen fermentation and methane reduction in Indonesian thin-tailed sheep: An in vitro study

Background and Aim: Seeds from Carica pubescens were observed to be abundant as waste. This waste contains active plant compounds whose utilization has not been studied in the livestock sector. This study was conducted to evaluate the potential of an aqueous seed extract of Carica pubescens (ASE) as an anthelmintic agent during rumen fermentation and methane reduction. Materials and Methods: Aqueous seed extract of Carica pubescens was prepared from C. pubescens cultivated in Wonosobo, Indonesia. Phytochemical analyses were performed to quantify the secondary metabolite content of ASE. In vitro adult worm mortality tests, scanning electron microscopy, and gas production tests were conducted to evaluate rumen characteristics, methane reduction, and the potential of ASE as an anthelmintic against Haemonchus contortus. Adult worms and ruminal fluid were collected from Indonesian thin-tailed sheep. Two-way analysis of variance followed by Tukey’s test was performed using the Statistical Package for the Social Sciences® 21.0 software to detect significant differences. Results: In vitro study results showed that 1–5% ASE inhibited H. contortus after the 1st h of incubation, and 5% ASE suppressed 100% of adult H. contortus worms in the 5th h. Scanning electron microscopy analysis of ASE-treated worms ASE revealed damaged cuticle structures. ASE had no significant effect on pH, NH3, volatile fatty acid, acetate, propionate, butyrate, acetate: propionate, or microbial protein in rumen fluid (p > 0.05). The in vitro feed fermentation results showed that ASE significantly affected methane reduction. Conclusion: The inclusion of up to 5% ASE in sheep diets may serve as a potential alternative anthelmintic against H. contortus as well as a methane reduction agent, without deleterious effects on rumen fermentation

Detection of Genes on Eschericia coli Producing Extended Spectrum β-lactamase Isolated from the Small Intestine of Ducks in Traditional Markets Surabaya City, Indonesia

This research aimed to focus on the presence of ESBL-producing E. coli isolated from the small intestine of ducks in several traditional markets in Surabaya as there is still little information regarding cases of ESBL-producing E. coli in ducks in Indonesia. Samples from the small intestine of ducks were kept on Buffer Peptone Water (BPW) media and then streaked on Eosin Methylene Blue Agar (EMBA) media for isolation testing, while Gram staining and IMViC tests were used to continue the identification test. Kirby-bauer diffusion test for determining antibiotic sensitivity and confirmation of ESBL producing E. coli using the double disc synergy test (DDST). Molecular detection of TEM and CTX-M genes was carried out using Polymerase Chain Reaction (PCR). Based on morphological culture characteristics, Gram staining, and biochemical testing, the sample analysis results revealed that 32 samples (32%) of the 100 samples that were isolated were confirmed to be positive for E. coli. 10 from 32 E. coli isolates (31.25%) were confirmed to be multidrug resistance (MDR) because they were resistant to 3 to 4 antibiotic classes. Two out of ten E. coli isolates reported to be multidrug resistant were discovered to have positive DDST test findings after the ESBL identification of those isolates. Based on molecular examination, one isolate of E. coli was found containing TEM and CTX-M genes. Giving antibiotics to ducks must be considered in the dose or level so as not to cause antibiotic residues in the digestive tract of ducks. More farmer awareness of and public concern for the safety of food of animal origin is required, as is the use of antibiotics in poultry under the supervision of a veterinarian.