119 research outputs found

    Saturation excess runoff numerical simulation

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    Saturation excess runoff is a relevant process which needs additional experimental and modeling efforts. This work is focused on its numerical modeling. The final objective is the successive interpretation of ongoing experimental monitoring results in two watersheds in different areas of Italy where the saturation excess runoff formation mechanism seems to be important. The numerical solution of the two-dimensional Richards\u2019 equation allows the evaluation of the sensitivity to the various influent parameters : rainfall intensity, soil properties, depth and initial water content, slope and hillslope length. Also the subsurface flow is simulated at the same time, allowing the evaluation of the different characteristic times and dominances of the two processes, namely subsurface and surface runoff. Only steady runoff intensities are considered for the sake of simplifying the results interpretation, but unsteady ones can be easily implemented. The same holds for soil layering

    Ambienti umidi effimeri e naturalità del paesaggio in Sicilia

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    This paper analyzes the relationship between vegetal communities conservation state and surrounding landscape naturalness. Expert-based assessment, based on phytosociological releves, has been used to define the qualitative condition of vegetal coenoses. Landscape naturalness has been assessed using Naturalness Evaluation Index (NEI). Analysis showed strong correlation between landscape naturalness and assemblages conservation state. Ephemeral wetlands survival is tied to proper conservation of surrounding territory

    Modelling the Frequency of Interarrival Times and Rainfall Depths with the Poisson Hurwitz-Lerch Zeta Distribution

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    The Poisson-stopped sum of the Hurwitz-Lerch zeta distribution is proposed as a model for interarrival times and rainfall depths. Theoretical properties and characterizations are investigated in comparison with other two models implemented to perform the same task: the Hurwitz-Lerch zeta distribution and the one inflated Hurwitz-Lerch zeta distribution. Within this framework, the capability of these three distributions to fit the main statistical features of rainfall time series was tested on a dataset never previously considered in the literature and chosen in order to represent very different climates from the rainfall characteristics point of view. The results address the Hurwitz-Lerch zeta distribution as a natural framework in rainfall modelling using the additional random convolution induced by the Poisson-stopped model as a further refinement. Indeed the Poisson contribution allows more flexibility and depiction in reproducing statistical features, even in the presence of very different climates

    Functional characterization of the sea urchin sns chromatin insulator in erythroid cells

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    Chromatin insulators are regulatory elements that determine domains of genetic functions. We have previously described the characterization of a 265 bp insulator element, termed sns, localized at the 3' end of the early historic H2A gene of the sea urchin Paracentrotus lividus. This sequence contains three cis-acting elements (Box A, Box B, and Box C+T) all needed for the enhancer-blocking activity in both sea urchin and human cells. The goal of this Study was to further characterize the sea urchin sns insulator in the erythroid environment. We employed colony assays in human (K562) and mouse (MEL) erythroid cell lines. We tested the capability of sns to interfere with the communication between the 5HS2 enhancer of the human beta-globin LCR and the gamma-globin promoter. We found that the sns sequence displays directional enhancer-blocking activity. By the use of antibodies against known DNA binding proteins, in electrophoretic mobility shift assays, we demonstrated the binding of the erythroid-specific GATA-1 and the ubiquitous Oct-1 and Sp1 transcription factors. These factors bind to Box A, Box B, and Box C+T, respectively, in both K562 and MEL nuclear extracts. These results may have significant implications for the conservation of insulator function ill evolutionary distant organisms and may prove to be of practical benefit in gene transfer applications for erythroid disorders such as hemoglobinopathies and thalassemias

    Statistical analysis of inter-arrival times of rainfall events for Italian Sub-Alpine and Mediterranean areas

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    Abstract. In this work a set of time-series of inter-arrival times of rainfall events, at daily scale, was analysed, with the aim to verify the issue of increasing duration of dry periods. The set consists of 12 time-series recorded at rain gauges in 1926–2005, six of them belong to an Italian Sub-Alpine area (Piedmont) and six to a Mediterranean one (Sicily). In order to overcome the problem related to limited sample size for high values of inter-arrival times, the discrete probability polylog-series distribution was used to fit the empirical data from partial (20 yr) time-series. Moreover, a simple qualitative trend analysis was applied to some high quantiles of inter-arrival times as well as to the average extent of rain clusters. The preliminary analysis seems to confirm the issue of increasing duration of dry periods for both environments, which is limited to the ''cold'' season

    Throughput and energy efficiency in IEEE 802.11 WLANs: friends or foes?

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    Proceedings of: 6th International ICST Conference on Heterogeneous Networking for Quality, Reliability, Security and Robustness, QShine 2009 and 3rd International Workshop on Advanced Architectures and Algorithms for Internet Delivery and Applications, AAA-IDEA 2009, Las Palmas, Gran Canaria, November 23-25, 2009Understanding and optimizing the energy consumption of wireless devices is critical to maximize network lifetime and to provide guidelines for the design of new protocols and interfaces. In this work we first provide an accurate analysis of the energy performance of an IEEE 802.11 WLAN, and then we derive the configuration to maximize it. We also analyze the impact of the energy configuration of the device on the throughput performance, and discuss in which circumstances throughput and energy efficiency can be both maximized and where they constitute different challenges.European Community's Seventh Framework ProgramPublicad

    Wharton’s Jelly Mesenchymal Stromal Cells Support the Expansion of Cord Blood–derived CD34 + Cells Mimicking a Hematopoietic Niche in a Direct Cell–cell Contact Culture System

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    Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) have been recently exploited as a feeder layer in coculture systems to expand umbilical cord blood–hematopoietic stem/progenitor cells (UCB-HSPCs). Here, we investigated the role of WJ-MSCs in supporting ex vivo UCB-HSPC expansion either when cultured in direct contact (DC) with WJ-MSCs or separated by a transwell system or in the presence of WJ-MSC–conditioned medium. We found, in short-term culture, a greater degree of expansion of UCB-CD34 + cells in a DC system (15.7 ± 4.1-fold increase) with respect to the other conditions. Moreover, in DC, we evidenced two different CD34 + cell populations (one floating and one adherent to WJ-MSCs) with different phenotypic and functional characteristics. Both multipotent CD34 + /CD38 − and lineage-committed CD34 + /CD38 + hematopoietic progenitors were expanded in a DC system. The former were significantly more represented in the adherent cell fraction than in the floating one (18.7 ± 11.2% vs. 9.7 ± 7.9% over the total CD34 + cells). Short-term colony forming unit (CFU) assays showed that HSPCs adherent to the stromal layer were able to generate a higher frequency of immature colonies (CFU-granulocyte/macrophage and burst-forming unit erythroid/large colonies) with respect to the floating cells. In the attempt to identify molecules that may play a role in supporting the observed ex vivo HSPC growth, we performed secretome analyses. We found a number of proteins involved in the HSPC homing, self-renewal, and differentiation in all tested conditions. It is important to note that a set of sixteen proteins, which are only in part reported to be expressed in any hematopoietic niche, were exclusively found in the DC system secretome. In conclusion, WJ-MSCs allowed a significant ex vivo expansion of multipotent as well as committed HSPCs. This may be relevant for future clinical applications

    Thermal spray coatings for corrosion and wear protection of naval Diesel engines components

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    Hard chrome plating has been widely used as the standard solution for valves stem, but its limited wear-corrosionresistance and the high toxicity of its galvanic baths suggest to look for suitable alternatives, such as thermalsprayed ceramic-metallic (cermet) and self-fluxing alloys.The present study aims to compare different solutions in terms of corrosion resistance under a selected acidenvironment and of wear resistance both for as-sprayed and post-corrosion samples.Coatings have been deposited by HVOF both on martensitic steel and Ni-based superalloy. A Design of Experimentprocedure has been used for spray parameters optimization, based on porosity and deposition efficiency ofthe coatings. Corrosion tests have shown the effect of porosity of the coatings, while wear tests confirmed thefundamental role of the dispersed hard phases.All the solutions here proposed have shown a significant improvement in terms of corrosion and wear resistancecompared to hard chrome plating
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