Applying RGB- and thermal-based vegetation indices from UAVs for high-throughput field phenotyping of drought tolerance in forage grasses

The persistence and productivity of forage grasses, important sources for feed production, are threatened by climate change-induced drought. Breeding programs are in search of new drought tolerant forage grass varieties, but those programs still rely on time-consuming and less consistent visual scoring by breeders. In this study, we evaluate whether Unmanned Aerial Vehicle (UAV) based remote sensing can complement or replace this visual breeder score. A field experiment was set up to test the drought tolerance of genotypes from three common forage types of two different species: Festuca arundinacea, diploid Lolium perenne and tetraploid Lolium perenne. Drought stress was imposed by using mobile rainout shelters. UAV flights with RGB and thermal sensors were conducted at five time points during the experiment. Visual-based indices from different colour spaces were selected that were closely correlated to the breeder score. Furthermore, several indices, in particular H and NDLab, from the HSV (Hue Saturation Value) and CIELab (Commission Internationale de l’éclairage) colour space, respectively, displayed a broad-sense heritability that was as high or higher than the visual breeder score, making these indices highly suited for high-throughput field phenotyping applications that can complement or even replace the breeder score. The thermal-based Crop Water Stress Index CWSI provided complementary information to visual-based indices, enabling the analysis of differences in ecophysiological mechanisms for coping with reduced water availability between species and ploidy levels. All species/types displayed variation in drought stress tolerance, which confirms that there is sufficient variation for selection within these groups of grasses. Our results confirmed the better drought tolerance potential of Festuca arundinacea, but also showed which Lolium perenne genotypes are more tolerant

Flux pinning by regular arrays of ferromagnetic dots

The pinning of flux lines by two different types of regular arrays of submicron magnetic dots is studied in superconducting Pb films; rectangular Co dots with in-plane magnetization are used as pinning centers to investigate the influence of the magnetic stray field of the dots on the pinning phenomena, whereas multilayered Co/Pt dots with out-of-plane magnetization are used to study the magnetic interaction between the flux lines and the magnetic moment of the dots. For both types of pinning arrays, matching anomalies are observed in the magnetization curves versus perpendicular applied field at integer and rational multiples of the first matching field, which correspond to stable flux configurations in the artificially created pinning potential. By varying the magnetic domain structure of the Co dots with in-plane magnetization, a clear influence of the stray field of the dots on the pinning efficiency is found. For the Co/Pt dots with out-of-plane magnetization, a pronounced field asymmetry is observed in the magnetization curves when the dots are magnetized in a perpendicular field prior to the measurement. This asymmetry can be attributed to the interaction of the out-of-plane magnetic moment of the Co/Pt dots with the local field of the flux lines and indicates that flux pinning is stronger when the magnetic moment of the dot and the field of the flux line have the same polarity.Comment: 7 pages including figures; submitted for publication in Physica C (Proceedings ESF-Vortex Conference, 18-24 Sept. 1999, Crete, Greece

Industrial chicory genome gives insights into the molecular timetable of anther development and male sterility

Industrial chicory (Cichorium intybus var. sativum) is a biannual crop mostly cultivated for extraction of inulin, a fructose polymer used as a dietary fiber. F1 hybrid breeding is a promising breeding strategy in chicory but relies on stable male sterile lines to prevent self-pollination. Here, we report the assembly and annotation of a new industrial chicory reference genome. Additionally, we performed RNA-Seq on subsequent stages of flower bud development of a fertile line and two cytoplasmic male sterile (CMS) clones. Comparison of fertile and CMS flower bud transcriptomes combined with morphological microscopic analysis of anthers, provided a molecular understanding of anther development and identified key genes in a range of underlying processes, including tapetum development, sink establishment, pollen wall development and anther dehiscence. We also described the role of phytohormones in the regulation of these processes under normal fertile flower bud development. In parallel, we evaluated which processes are disturbed in CMS clones and could contribute to the male sterile phenotype. Taken together, this study provides a state-of-the-art industrial chicory reference genome, an annotated and curated candidate gene set related to anther development and male sterility as well as a detailed molecular timetable of flower bud development in fertile and CMS lines

Morphological and pathogenic characterization of genetically diverse Sclerotinia isolates from European red clover crops (Trifolium pratense L.)

Clover rot, an important disease in European red clover crops, is caused by Sclerotinia trifoliorum or Sclerotinia sclerotiorum. Until today, little is known about the variation in aggressiveness among Sclerotinia isolates from red clover. Aggressiveness has never been correlated with morphological characteristics. Rapidly growing isolates may be more aggressive, but this was never investigated in S. trifoliorum before. Also nothing is known about the link between sclerotia production and aggressiveness. Oxalic acid is an important pathogenicity factor in Sclerotinia species, but its effect on aggressiveness is unknown in S. trifoliorum isolates. For this study, we selected 30 Sclerotinia isolates from 25 locations Europe: 26 S. trifoliorum isolates and 4 S. sclerotiorum isolates from two locations in France (Fr.A and Fr.B). For each isolate, the in vitro growth speed, sclerotia production, oxalate production and aggressiveness were analysed and correlations were estimated between aggressiveness and the other characteristics. Aggressiveness was assessed in vitro on detached leaves and in a greenhouse on young plants. Our isolates differed significantly in growth speed, sclerotia production, oxalate production and aggressiveness. The infections on detached leaves and young plants revealed interaction between isolates and plant genotypes and between isolates and cultivars, but there was no indication that pathotypes exist. In vitro growth speed and in vitro aggressiveness on detached leaves were positively correlated with aggressiveness on young plants, while sclerotia production was negatively correlated with aggressiveness on young plants. These factors can be used as predictors of aggressiveness of Sclerotinia isolates from red clover crops

Aggressiveness study on Sclerotinia isolates from red clover crops

Sclerotinia trifoliorum Erikks. causes clover rot (clover cancer, Sclerotinia crown and root rot) in red clover crops (Trifolium pratense L.), an important disease in Europe. Little is known about the aggressiveness of Sclerotinia isolates and aggressiveness studies were never conducted on a European scale. In this study we compared the aggressiveness of 30 Sclerotinia isolates isolated from red clover crops in 25 locations in 12 European countries using a plant-based bio-test. Plants from 6 red clover cultivars with different resistance levels were spray inoculated at the age of 12 weeks with 1 to 1.5 ml mycelium fragment suspension per plant. After 10 days incubation, plants were scored on a scale from 1 (healthy plant) to 5 (dead plant) and the disease index was calculated. The experiment was repeated 3 times and all repetitions were highly correlated. Average disease indices ranged from 52.6% to 82.7%. Significant differences were detected between isolates and between cultivars, but there was no isolate – cultivar interaction. Based on these results, the most aggressive isolates can be selected for resistance breeding. Future work should investigate whether the differences in aggressiveness are due to a higher growth speed or due to a higher secretion of cell-wall degrading components and pathogenicity factors

Breeding in a world of scarcity : proceedings of the 2015 meeting of the section 'Forage Crops and Amenity Grasses' of Eucarpia

B

Resistance of red clover to broad spectrum of Sclerotinia trifoliorum

In this research a diversity study on different European isolates of the pathogenic fungus S. trifoliorum will be performed using mycelial compatibility grouping and AFLP. The next step is the development of a bio-test to screen red clover plants for their resistance level against clover rot. The third step in this research is evaluating different European strains for their virulence and evaluating a broad spectrum of red clover varieties for their resistance against S. trifoliorum. Over 100 varieties will be evaluated, including cultivars, landraces and wild varieties. Finally the inheritage of clover rot resistance in red clover will be evaluated by a QTL study. Sclerotinia isolates have been collected from clover fields among different European countries. Mycelial compatibility has shown a large variability within fields. The DNA extraction has been optimized. Sequencing of the ITS-region will be used to determine the exact species of every isolate. Primer combinations are currently being tested for the AFLP study. Different culture media were tested for their capacity to induce the production of multiple big sclerotia. The most optimal medium is being used to produce sclerotia from every isolate. Sclerotia are induced to apothecia formation and formed ascospores will be used to construct the bio-test

Tall fescue and Italian ryegrass: an ideal mixture for intensive cutting management

We established a trial in 2009 to compare the agronomic performance of tall fescue (Festuca arundinacea, Fa) with Italian ryegrass (Lolium multiflorum, Lm) growing in monoculture and in different mixtures of both species. Mixtures differed in the ploidy of the Italian ryegrass component (diploid vs. tetraploid) and the contribution of both species to the mixtures (1/4 or 1/8 of Lm seeds on a number base). Five cuts were taken in both 2010 and 2011. N-fertilization was around 300 kg N ha–1 per year. Dry matter (DM) content and botanical composition and feeding value were determined at each cut. Lm outyielded Fa in the first year after sowing but Fa outyielded Lm in the second year. In one of the mixtures, transgressive overyielding occurred in both years. The proportion of Fa in the DM yield of the mixtures was initially much lower than the sown proportion but increased gradually. We conclude that mixtures of Fa and Lm are particularly suited for an intensive cutting management