Автоматизация бизнес-процесса «Управление капитальным строительством» в части формирования отчетности

Автоматизация бизнес-процесса "Управление капитальным строительством" в части формирования отчетности раздела 3 подразделов "В, Г, Д" документа "Отчет агента" . Проведен анализ бизнес-процесса "Управление капитальным строительством", его автоматизация. Доработана система формирования отчетности, разработаны формы документов, регистры накопления и сведений.Automation of the business process "Capital Construction Management" in terms of reporting in section 3 of subsections "C, D, Е" of the document "Agent Report". The analysis of the business process "Capital Construction Management" automated. The reporting system has been improved, forms of documents, accumulation and information registers have been developed

Targeted tumor therapy with a fusion Protein of an antiangiogenic human recombinant scFv and yeast cytosine deaminase

In adults, endothelial cell division occurs only in wound healing, during menstruation, or in diseases such as wet age-related macular degeneration or development of benign or malignant tissues. Angiogenesis is one of the major requirements to supply the fast developing tumor tissue with oxygen and nutrients, and enables it to spread into other tissues far from its origin. We selected the extradomain B (ED-B), a splice variant of fibronectin, which is exclusively expressed in ovaries, uterus, during wound healing, and in tumor tissues, as a target for the development of an innovative antiangiogenic, prodrug-based targeted tumor therapy approach. We designed a fusion protein termed L19CDy-His, consisting of the antibody single chain fragment L19 for targeting ED-B and yeast cytosine deaminase for the conversion of 5-fluorocytosine into cytotoxic 5-fluorouracil. We purified high amounts of the fusion protein from Pichia pastoris that is stable, enzymatically active, and retains 75% of its activity after incubation with human plasma for up to 72 hours. The binding of L19CDy-His to ED-B was confirmed by an enzyme-linked immunosorbent assay and quantified by surface plasmon resonance spectroscopy determining a KD value of 81±7 nM. L19CDy-His successfully decreased cell survival of the murine ED-B-expressing teratocarcinoma cell line F9 upon addition of the prodrug 5-fluorocytosine. Our data demonstrate the suitability of targeting ED-B by L19CDy-His for effective prodrug-based tumor therapy

Mehrrobotersysteme in der vernetzten Operationsführung

Die Vernetzte Operationsführung dient der Führung und dem Einsatz von Streitkräften auf der Basis eines streitkräftegemeinsamen, führungsübergreifenden und interoperablen Kommunikations- und Informationsverbundes. Wesentliche Bestandteile der Vernetzten Operationsführung sind Kommunikations- und Sensornetze. Für den Einsatz von Mehrrobotersystemen ist die Integration in die vernetzte Operationsführung von entscheidender Bedeutung. Als Grundlage für die Integration ist jedoch ein geeignetes Kommunikationsnetz notwendig. Es werden daher zunächst basierend auf dem NATO-Workshop „1st NATO Workshop on short-term realizable (multi-)robot systems in military domains“ die Kommunikationsanforderungen an ein Mehrrobotersystem identifiziert und Schwierigkeiten bei der Umsetzung in einem Kommunikationssystem diskutiert. Anschließend wird mit dem Protokoll WNet ein flexibler Rahmen vorgestellt, um Lösungen für Kommunikationssysteme zu untersuchen, die möglichst viele dieser Anforderungen berücksichtigen. Die zusätzliche oder detailliertere Berücksichtigung von Anforderungen kann dabei durch Erweiterungen des Rahmens vorgenommen werden. WNet ist somit ein experimenteller Protokollrahmen, um unterschiedliche Verfahren zur Kommunikation in Mehrrobotersystemen zu vergleichen. Das Ergebnis dieser Vergleiche soll dann die Grundlage für ein oder mehrere geeignete Kommunikationssysteme für Mehrrobotersysteme bilden, die eine einfache Integration in die vernetzte Operationsführung erlauben

Label-Free Immunodetection in High Ionic Strength Solutions Using Carbon Nanotube Transistors with Nanobody Receptors

Nanomaterial-based field-effect transistors (FETs) have been proposed for real-time, label-free detection of various biological species. However, screening of the analyte charge by electrolyte ions (Debye screening) has so far limited their use in physiological samples. Here, this challenge is overcome by combining FETs based on single-walled semiconducting carbon nanotube networks (SWCNTs) with a novel surface functionalization comprising: (1) short nanobody receptors, and (2) a polyethylene glycol layer (PEG). Nanobodies are stable, easy-to-produce, short biological receptors (~2–4 nm) that enable analyte binding closer to the sensor surface. The addition of PEG enhances the signal in high ionic strength environment. Using green fluorescent protein (GFP) as a model antigen, high selectivity and sub-picomolar detection limit with a dynamic range exceeding 4 orders of magnitude is demonstrated in physiological solutions. The presented immunoassay is fast, label-free, does not require any sample pre-treatment or washing steps