6 research outputs found
In vitro susceptibility of coliform and Pseudomonasspecie to some antibiotics by E-test strips
The aim of this study was to characterize the in vitro antimicrobial susceptibility profile of Coliform and
Pseudomonas species strains to 10 antibiotics (Vancomycine, Ciprofloxine, Tetracyclin,
Chloramphenicol , Erythromycin, Streptomycine, Gentamycine , Ampicilline, trimethoprimsulfamethoxazole
and Clarithromycin) that could potentially be used in clinical therapy.
MIC was determined for antibiotics in Mueller-Hinton (MH) agar plates, by using E-test strips
(Liofilchem, Roseto degli Abruzzi, Italy). MIC was read after 24 h of incubation at 37°C.
The both strains were more susceptible to Ciprofloxine (MIC values: Coliform, 0.002 μg/mL; and
Pseudomonas, 0.047 μg/mL). No strain of Pseudomonas was found sensitive to Chloramphenicol,
Erythromycin, Ampicilline, trimethoprim-sulfamethoxazole and Clarithromycin, contrary to coliform,
which was found resistant to those antibiotics with MIC values ranged between 0.38 and 128 μg/mL .
Gentamycine (MIC values: Coliform, 0.125μg/mL; and Pseudomonas, 0.50 μg/mL), Tetracyclin (MIC
values: Coliform, 1.00 μg/mL; and Pseudomonas, 0.75 μg/mL) and Streptomycine (MIC values:
Coliform, 0.75 μg/mL; and Pseudomonas, 2.5 μg/mL) were also active against Coliform and
Pseudomonas strains, while resistance to Vancomycine (no inhibition) was observed in the two strains