Investigation of osteoblast response to biodegradable bacterial cellulose scaffolds

[Excerpt] Aim of this project is to investigate the ability of bacterial cellulose (BC) and oxidized Bacterial cellulose (OBC) use as a scaffold in tissue engineering. Bacterial cellulose was produced with being provided optimum conditions from Acetobacter xylinus. BC was transformed dialdehyde cellulose (DAC) as biodegradable form by treating with periodate. Oxidation was carried out in aqueous solution at 508C in the dark for 24 hours. The mole-to-mole ratio of sodium metaperiodate to anhydroglucose repeat unit (AGU) of cellulose was 0.5, 1.0 and 1.5. [...]info:eu-repo/semantics/publishedVersio

A novel biodegradable PCL film for tendon reconstruction: Achilles tendon defect model in rats

This study aims to investigate applicability of poly(E-caprolactone) (PCL) biodegradable films for repair of gaps in Achilles tendons in a rat model, also comparing surgical repair versus no repair approaches. PCL was synthesized with tailor-made properties, then, PCL films were prepared by solvent casting. Seventy-five outbred Sprague-Dawley rats were randomly allocated into five groups: (i) sham operated (skin incision only); (ii) no repair (complete division of the Achilles tendon and plantaris tendon without repair); (iii) Achilles repair (with a modified Kessler type suture); and (iv) plasty of Achilles tendon defects with the biodegradable PCL films, and (v) animals subjected to 1cm, mid-substance defect with no repair. Functional performance was determined from the measurements of hindpaw prints utilizing the Achilles functional index. The animals were killed 8 weeks after surgery and histological and biomechanical evaluations were made. All groups subjected to Achilles tendon division had a significant functional impairment that gradually improved so that by day 28 there were no functional impairments in any group whereas animals with a defect remained impaired. The magnitude of the biomechanical and morphological changes at postoperative 8 weeks were similar for no repair group (conservative), Achilles repair group and tendonplasty group (biodegradable PCL film group). The initial rate of functional recovery was significantly different for primary suture, Achilles repair group and PCL film group (p>0.01). But, at the 28th day, functional recovery was quite similar to the other groups. In summary, our results suggest that the PCl film can be an alternative biomaterial for tendon replacement

Molecular dynamics simulation of classical thermosize effects

We present the first molecular dynamics simulations of classical thermosize effects for realistic molecular conditions and flows. The classical thermosize effect is the chemical potential difference induced between two different-sized channels that have different fluid transport processes. It can be generated by applying a temperature gradient within the different-sized domains, and in this article the system investigated is a combination of a microchannel and a nanochannel. Our molecular dynamics results are compared with a theoretical calculation of the induced chemical potential difference, and this yields useful new insight into diffusive transport in nonequilibrium gas flows