On a classical spectral optimization problem in linear elasticity

We consider a classical shape optimization problem for the eigenvalues of elliptic operators with homogeneous boundary conditions on domains in the $N$-dimensional Euclidean space. We survey recent results concerning the analytic dependence of the elementary symmetric functions of the eigenvalues upon domain perturbation and the role of balls as critical points of such functions subject to volume constraint. Our discussion concerns Dirichlet and buckling-type problems for polyharmonic operators, the Neumann and the intermediate problems for the biharmonic operator, the Lam\'{e} and the Reissner-Mindlin systems.Comment: To appear in the proceedings of the workshop `New Trends in Shape Optimization', Friedrich-Alexander Universit\"{a}t Erlangen-Nuremberg, 23-27 September 201

Analyticity and criticality results for the eigenvalues of the biharmonic operator

We consider the eigenvalues of the biharmonic operator subject to several homogeneous boundary conditions (Dirichlet, Neumann, Navier, Steklov). We show that simple eigenvalues and elementary symmetric functions of multiple eigenvalues are real analytic, and provide Hadamard-type formulas for the corresponding shape derivatives. After recalling the known results in shape optimization, we prove that balls are always critical domains under volume constraint.Comment: To appear on the proceedings of the conference "Geometric Properties for Parabolic and Elliptic PDE's - 4th Italian-Japanese Workshop" held in Palinuro (Italy), May 25-29, 201

Conformational altered p53 affects neuronal function: relevance for the response to toxic insult and growth-associated protein 43 expression

The role of p53 in neurodegenerative diseases is essentially associated with neuronal death. Recently an alternative point of view is emerging, as altered p53 conformation and impaired protein function have been found in fibroblasts and blood cells derived from Alzheimer's disease patients. Here, using stable transfected SH-SY5Y cells overexpressing APP751wt (SY5Y-APP) we demonstrated that the expression of an unfolded p53 conformation compromised neuronal functionality. In particular, these cells showed (i) augmented expression of amyloid precursor protein (APP) and its metabolites, including the C-terminal fragments C99 and C83 and β-amyloid peptide (ii) high levels of oxidative markers, such as 4-hydroxy-2-nonenal Michael-adducts and 3-nitro-tyrosine and (iii) altered p53 conformation, mainly due to nitration of its tyrosine residues. The consequences of high-unfolded p53 expression resulted in loss of p53 pro-apoptotic activity, and reduction of growth-associated protein 43 (GAP-43) mRNA and protein levels. The role of unfolded p53 in cell death resistance and lack of GAP-43 transcription was demonstrated by ZnCl(2) treatment. Zinc supplementation reverted p53 wild-type tertiary structure, increased cells sensitivity to acute cytotoxic injury and GAP-43 levels in SY5Y-APP clone

SMART-1 Impact Ground-based campaign

Based on predictions of impact magnitude and cloud ejecta dynamics, we organized a SMART-1 ground-based observation campaign to perform coordinated measurements of the impact. Results from the coordinated multi-site campaign will be discussed

Select2Plan: training-free ICL-based planning through VQA and memory retrieval

We introduce Select2Plan (S2P), a novel training-free framework for high-level robot planning that leverages off-the-shelf VLMs for autonomous navigation. Unlike most learning-based approaches that require extensive task-specific training and large-scale data collection, S2P overcomes the need for fine-tuning by adapting inputs to align with the VLM's pretraining data. Our method achieves this through a combination of structured Visual Question Answering (VQA) to ground action selection on the image, and In-Context Learning (ICL) to exploit knowledge drawn from relevant examples from a memory bank of (visually) annotated data, which can include diverse, in-the-wild sources. We demonstrate S2P flexibility by evaluating it in both First-Person View (FPV) and Third-Person View (TPV) navigation. S2P improves the performance of a baseline VLM by 40% in TPV and surpasses end-to-end trained models by approximately 24% in FPV when tasked with navigating towards unseen objects in novel scenes. These results highlight the adaptability, simplicity, and effectiveness of our training-free approach, demonstrating that the use of pre-trained VLMs with structured memory retrieval enables robust high-level robot planning without costly task-specific training. Our experiments also show that retrieving samples from heterogeneous data sources, including online videos of different robots or humans walking, is highly beneficial for navigation. Notably, our method effectively generalizes to novel scenarios, requiring only a handful of demonstrations. Project Page: lambdavi.github.io/select2pla

Microfluidic manufacturing of tioconazole loaded keratin nanocarriers: Development and optimization by design of experiments

Fungal infections of the skin, nails, and hair are a common health concern affecting a significant proportion of the population worldwide. The current treatment options include topical and systematic agents which have low permeability and prolonged treatment period, respectively. Consequently, there is a growing need for a permeable, effective, and safe treatment. Keratin nanoparticles are a promising nanoformulation that can improve antifungal agent penetration, providing sustainable targeted drug delivery. In this study, keratin nanoparticles were prepared using a custom-made 3D-printed microfluidic chip and the manufacturing process was optimized using the design of experiments (DoE) approach. The total flow rate (TFR), flow rate ratio (FRR), and keratin concentration were found to be the most influential factors of the size and polydispersity index (PDI) of the nanoparticles. The crosslinking procedure by means of tannic acid as safe and biocompatible compound was also optimized. Keratin nanoparticles loaded with a different amount of tioconazole showed a size lower than 200 nm, a PDI lower than 0.2 and an encapsulation efficiency of 91 ± 1.9 %. Due to their sustained drug release, the formulations showed acceptable in vitro biocompatibility. Furthermore, a significant inhibitory effect compared to the free drug against Microsporum canis

Nitrogen nutrition and xylem sap composition in Zea mays: effect of urea, ammonium and nitrate on ionomic and metabolic profiles

In plants the communication between organs is mainly carried out via the xylem and phloem. The concentration and the molecular species of some phytohormones, assimilates and inorganic ions that are translocated in the xylem vessel play a key role in the systemic nutritional signaling in plants. In this work the composition of the xylem sap of maize was investigated at the metabolic and ionomic level depending on the N form available in the nutrient solution. Plants were grown up to 7 days in hydroponic system under N-free nutrient solution or nutrient solution containing N in form of nitrate, urea, ammonium or a combination of urea and ammonium. For the first time this work provides evidence that the ureic nutrition reduced the water translocation in maize plants more than mineral N forms. This result correlates with those obtained from the analyses of photosynthetic parameters (stomatal conductance and transpiration rate) suggesting a parsimonious use of water by maize plants under urea nutrition. A peculiar composition in amino acids and phytohormones (i.e. S, Gln, Pro, ABA) of the xylem sap under urea nutrition could explain differences in xylem sap exudation in comparison to plants treated with mineral N forms. The knowledge improvement of urea nutrition will allow to further perform good agronomic strategies to improve the resilience of maize crop to water stress

Increased susceptibility to Chrysanthemum Yellows phytoplasma infection in Atcals7ko plants is accompanied by enhanced expression of carbohydrate transporters

Main conclusion: Loss of CALS7 appears to confer increased susceptibility to phytoplasma infection in Arabidopsis, altering expression of genes involved in sugar metabolism and membrane transport. Abstract: Callose deposition around sieve pores, under control of callose synthase 7 (CALS7), has been interpreted as a mechanical response to limit pathogen spread in phytoplasma-infected plants. Wild-type and Atcals7ko mutants were, therefore, employed to unveil the mode of involvement of CALS7 in the plant’s response to phytoplasma infection. The fresh weights of healthy and CY-(Chrysanthemum Yellows) phytoplasma-infected Arabidopsis wild type and mutant plants indicated two superimposed effects of the absence of CALS7: a partial impairment of photo-assimilate transport and a stimulated phytoplasma proliferation as illustrated by a significantly increased phytoplasma titre in Atcal7ko mutants. Further studies solely dealt with the effects of CALS7 absence on phytoplasma growth. Phytoplasma infection affected sieve-element substructure to a larger extent in mutants than in wild-type plants, which was also true for the levels of some free carbohydrates. Moreover, infection induced a similar upregulation of gene expression of enzymes involved in sucrose cleavage (AtSUS5, AtSUS6) and transmembrane transport (AtSWEET11) in mutants and wild-type plants, but an increased gene expression of carbohydrate transmembrane transporters (AtSWEET12, AtSTP13, AtSUC3) in infected mutants only. It remains still unclear how the absence of AtCALS7 leads to gene upregulation and how an increased intercellular mobility of carbohydrates and possibly effectors contributes to a higher susceptibility. It is also unclear if modified sieve-pore structures in mutants allow a better spread of phytoplasmas giving rise to higher titre

Evaluation of a large set of patients with Autoimmune Polyglandular Syndrome from a single reference centre in context of different classifications

Purpose: To characterize patients with APS and to propose a new approach for their follow-up. Query ID="Q1" Text="Please check the given names and familynames." Methods: Monocentric observational retrospective study enrolling patients referred to the Outpatients clinic of the Units of Endocrinology, Diabetology, Gastroenterology, Rheumatology and Clinical Immunology of our Hospital for Autoimmune diseases. Results: Among 9852 patients, 1174 (11.9%) [869 (73.9%) female] were diagnosed with APS. In 254 subjects, the diagnosis was made at first clinical evaluation (Group 1), all the other patients were diagnosed with a mean latency of 11.3 ± 10.6 years (Group 2). Group 1 and 2 were comparable for age at diagnosis (35.7 ± 16.3 vs. 40.4 ± 16.6 yrs, p =.698), but different in male/female ratio (81/173 vs 226/696, p =.019). In Group 2, 50% of patients developed the syndrome within 8 years of follow-up. A significant difference was found after subdividing the first clinical manifestation into the different outpatient clinic to which they referred (8.7 ± 8.0 vs. 13.4 ± 11.6 vs. 19.8 ± 8.7 vs. 7.4 ± 8.1 for endocrine, diabetic, rheumatologic, and gastroenterological diseases, respectively, p <.001). Conclusions: We described a large series of patients affected by APS according to splitters and lumpers. We propose a flowchart tailored for each specialist outpatient clinic taking care of the patients. Finally, we recommend regular reproductive system assessment due to the non-negligible risk of developing premature ovarian failure