Estado e agricultores familiares: uma análise interpretativa sobre o desenvolvimento rural no Sul de Minas Gerais.

Este trabalho procurou identificar como os atores sociais vinculados à agricultura familiar (lideranças de produtores familiares, profissionais de ciências agrárias, autoridades municipais, pesquisadores e professores universitários) interpretam o papel do Estado para o desenvolvimento dessa categoria na região sul de Minas Gerais. Com relação à coleta de dados, utilizou-se a técnica "focused interview". Os entrevistados foram seledonados pelo método não-probabilístico de amostragem por julgamento. Os resultados revelaram que a interpretação feita pelos entrevistados aproxima-se mais da perspectiva do Estado patrimonialistaburocrático autoritário, tendo em vista que os depoimentos dos entrevistados corroboram com a reprodução das relações de dependência dos agricultores familiares em relação ao Estado. Por outro lado, alguns atores reconhecem a necessidade de mecanismos que incentivem a participação social, no entanto, salientaram que a mobilização dos produtores familiares na região ainda é incipiente e desarticulada

Le corpus des inscriptions grecques et latines de Philippes : apports récents et perspectives de recherche sur une colonie romaine d’Orient

Brélaz Cédric, Zannis Angelos G. Le corpus des inscriptions grecques et latines de Philippes : apports récents et perspectives de recherche sur une colonie romaine d’Orient. In: Comptes rendus des séances de l'Académie des Inscriptions et Belles-Lettres, 158e année, N. 4, 2014. pp. 1463-1507

De nouveaux notables dans la colonie de Philippes

Οι συγγραφείς παρουσιάζουν την προκαταρκτική δημοσίευση επτά ανέκδοτων επιγραφών, οι οποίες προέρχονται από τη ρωμαϊκή αποικία των Φιλίππων στην επαρχία της Μακεδονίας. Οι επιγραφές αυτές αποκαλύπτουν νέους τοπικούς προύχοντες, οι οποίοι έδρασαν στην αποικία κατά τη διάρκεια του 1ου και του 2ου αι. μ.Χ. Ανάμεσά τους υπάρχει ένας στρατιώτης από τους Φιλίππους, ο οποίος θήτευσε στις κοόρτεις των νυκτοφυλάκων στη Ρώμη, καθώς και πολλοί δημοτικοί άρχοντες και απελεύθεροι από τους Φιλίππους. Όλες οι επιγραφές που σχετίζονται με τους θεσμούς της αποικίας αλλά και αυτές που αναφέρουν μέλη των τάξεων των συγκλητικών και των ιππέων καθώς και στρατιώτες, θα επαναδημοσιευτούν σύντομα στο πρώτο τεύχος του Corpus des inscriptions grecques et latines de Philippes. H συγκέντρωση του επιγραφικού υλικού, το οποίο σχετίζεται με τη δημοτική και την αυτοκρατορική διοίκηση των Φιλίππων, εμπλουτισμένο με πολυάριθμες ανέκδοτες επιγραφές, θα οδηγήσει στην ανανέωση των γνώσεών μας για την κοινωνική και πολιτική δομή της αποικίας.The authors present the preliminary publication of seven hitherto unpublished inscriptions from the Roman colony at Philippi in the province of Macedonia. These inscriptions make known a number of new notables who were active in the colony during the 1st and 2nd centuries AD. Amongst them is a soldier from Philippi who had pursued a career in the cohortes vigilae in Rome, as well as several municipal magistrates and seviri augusti from Philippi. All the inscriptions connected with the institutions of the colony, and also those mentioning members of the senatorial and equestrian orders and soldiers, will shortly be included in the first volume of the Corpus of Greek and Latin Inscriptions of Philippi. The assembling of the epigraphic material relating to the municipal and imperial administration of Philippi, enriched by numerous unpublished inscriptions, will enable us to renew our acquaintance with the social and political structures of the colony.Les auteurs présentent la publication préliminaire de sept inscriptions inédites provenant de la colonie romaine de Philippes dans la province de Macédoine. Ces inscriptions font connaître de nouveaux notables locaux actifs dans la colonie au cours du Ier et du IIe siècles apr. J.-C. On note parmi eux un soldat originaire de Philippes ayant fait carrière dans les cohortes de vigiles à Rome, ainsi que plusieurs magistrats municipaux et sévirs augustaux philippiens. Toutes les inscriptions ayant trait aux institutions de la colonie, en même temps que celles mentionnant des membres des ordres sénatorial et équestre ainsi que des soldats, seront reprises prochainement dans le premier volume du Corpus des inscriptions grecques et latines de Philippes. Le rassemblement du matériel épigraphique relatif à l'administration municipale et impériale à Philippes, enrichi de nombreux inédits, permettra de renouveler notre connaissance des structures sociales et politiques de la colonie.Brélaz Cédric, Frei-Stolba Regula, Rizakis Athanassios, Zannis Angelos G. De nouveaux notables dans la colonie de Philippes. In: Bulletin de correspondance hellénique. Volume 130, livraison 1, 2006. pp. 519-547

Relative Generic Computational Forensic Techniques

Abstract. Computational forensic engineering is the process of identification of the tool or algorithm that was used to produce a particular output or solution by examining the structural properties of the output. We introduce a new Relative Generic Forensic Engineering (RGFE) technique that has several advantages over the previously proposed approaches. The new RGFE technique not only performs more accurate identification of the tool used but also provides the identification with a level of confidence. Additionally, we introduce a generic formulation (integer linear programming formulation) which enables rapid application of the RGFE approach to a variety of problems that can be formulated as 0-1 integer linear programs. The key innovations of the RGFE technique include the development of a simulated annealing-based (SA) CART classification technique and a generic property formulation technique that facilitates property reuse. We introduce instance properties which enable an enhanced classification of problem instances leading to a higher accuracy of algorithm identification. Finally, the single most important innovation, property calibration, interprets the value for a given algorithm for a given property relative to the values for other algorithms. We demonstrated the effectiveness of the RGFE technique on the boolean satisfiability (SAT) and graph coloring (GC) problems.

Trypanosoma cruzi cell death induced by the Morita-Baylis-Hillman adduct 3-Hydroxy-2-methylene-3-(4-nitrophenylpropanenitrile)

Submitted by Kamylla Nascimento ([email protected]) on 2017-11-27T11:36:23Z No. of bitstreams: 1 art. Trypanosoma cruzi - sandes.pdf: 5943676 bytes, checksum: c33130d68adc83b9c57c45ec2b59ed34 (MD5)Approved for entry into archive by Kamylla Nascimento ([email protected]) on 2017-11-27T11:57:20Z (GMT) No. of bitstreams: 1 art. Trypanosoma cruzi - sandes.pdf: 5943676 bytes, checksum: c33130d68adc83b9c57c45ec2b59ed34 (MD5)Made available in DSpace on 2017-11-27T11:57:20Z (GMT). No. of bitstreams: 1 art. Trypanosoma cruzi - sandes.pdf: 5943676 bytes, checksum: c33130d68adc83b9c57c45ec2b59ed34 (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Biofísica e Radiobiologia. Recife, PE, Brazil.Fundação Oswaldo Cruz. Centro Pesquisas Gonçalo Moniz. Laboratório de Biologia Parasitaria. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal da Paraíba. Departamento de Química. João Pessoa, PB, Brasil.Universidade Federal da Paraíba. Departamento de Química. João Pessoa, PB, Brasil.Universidade Federal da Paraíba. Departamento de Química. João Pessoa, PB, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Chagas disease, caused by the protozoan Trypanosoma cruzi, remains a serious health concern due to the lack of effective vaccines or satisfactory treatment. In the search for new compounds against this neglected disease, we have previously demonstrated that the compound 3-Hydroxy-2-methylene-3-(4-nitrophenylpropanenitrile) (MBHA3), derived from the Morita-Baylis-Hillman reaction, effectively caused a loss of viability in both the epimastigote and trypomastigote forms. However, the mechanisms of parasite death elicited by MBHA3 remain unknown. The aim of this study was to better understand the morphophysiological changes and the mechanism of cell death induced by MBHA3 treatment on T. cruzi. To perform this analysis, we used confocal microscopy and flow cytometry to monitor the fluorescent probes such as annexin-V/propidium iodide (AV/PI), calcein-AM/ethidium homodimer (CA/EH), acridine orange (AO) and rhodamine 123 (Rho 123). Lower concentrations of MBHA3 led to alterations in the mitochondrial membrane potential and AO labeling, but did not decrease the viability of the epimastiogote forms, as determined by the CA/EH and AV/PI assays. Conversely, treatment with higher concentrations of MBHA3 led to extensive plasma membrane damage, loss of mitochondrion membrane potential, DNA fragmentation and acidification of the cytoplasm. Our findings suggest that at higher concentrations, MBHA3 induces T. cruzi epimastigote death by necrosis in a mitochondrion-dependent manner

CD4+CD45RA−FOXP3low Regulatory T Cells as Potential Biomarkers of Disease Activity in Systemic Lupus Erythematosus Brazilian Patients

Heren, we analyzed Treg cells as potential biomarkers of disease activity in systemic lupus erythematosus (SLE) patients. Peripheral blood mononuclear cells from 30 SLE patients (15 active: SLEDAI > 6/15 SLE remission: SLEDAI< 6) and 15 healthy volunteers were purified. Treg immunophenotyping was performed using CD4, CD25, CD45, CD127, and FOXP3 markers. CD4+FOXP3+ Treg activation state was investigated based on CD45RA and FOXP3 expression. To increase the accuracy of our findings, a multivariate linear regression was performed. We showed a significant increase in the frequency of CD4+FOXP3+ Treg cells in SLE patients. However, unlike all other Treg cells phenotypes analyzed, only eTreg (CD4+FOXP3highCD45RA-) (p=0.01) subtype was inversely correlated with disease activity while Foxp3+nontreg (CD4+FOXP3lowCD45RA-) (p=0.003) exerted a direct influence in the outcome of the disease. Foxp3+nontreg cells were the most consistent SLE active indicator, confirmed by multiple linear regression analyses. In summary, our results demonstrate Foxp3+nontreg cells as new biomarkers in the search of an effective therapeutic strategy in SLE

Effects of MBHA3 treatment on the acidic compartments of T. cruzi.

<p>(A) Overlay flow cytometric histograms of the control and treated-cells labeled with AO, after 72 hours of drug incubation. A gradual shift of the red fluorescence could be observed in the cells treated with the IC₅₀ and the 2x IC₅₀ of MBHA3. Nevertheless, in cells treated with the 4x IC₅₀ of MBHA3, a striking decrease of the fluorescent signal from red channel was observed. (B–E). Confocal microscopy images of the control (B) and treated-cells (C–E). Control cells presented a normal morphology with a bright green nucleus, pale green cytoplasm and large red-labeled compartments at the posterior end of cells (B). Detail of the IC₅₀-treated culture showing slight changes in the parasite nucleus. (C). Aspect of parasite culture treated with the 2x IC₅₀ of MBHA3 showing severe acidification of the parasite cytoplasm (D). Note the presence of the pyknotic nucleus (white arrow, inset). Parasites treated with the 4x IC₅₀ of MBHA3 showed a round-shape body and a decrease in both green and red AO fluorescent signals (E).</p

DNA fragmentation induced by MBHA3 on <i>T. cruzi</i> epimastigote forms.

<p>Electrophoresis of total DNA of the control and MBHA3-treated epimastigote forms. Non-specific DNA fragmentation could be observed in cells treated with the 2x IC₅₀ and the 4x IC₅₀ MBHA3. H₂O₂ was used as a positive control of parasite death. M = Low DNA Mass Ladder.</p

Cytometry analysis of the effects of MBHA3 on Calcein AM/EH.

<p>Flow cytometric dot plots of control (A) and MBHA3-treated cells (B – D) labelled with CA/EH. Cells in the upper left quadrant were positive only for calcein. Cells in the lower right quadrant were positive only for ethidium heterodimer. Events in the upper right quadrant were double positive for both markers, whereas the lower left quadrant corresponds to the cells negative for the both dyes. The dot plots are representative of duplicate experiments with similar results. There were 20,000 recorded events/experimental condition.</p