Chikungunya Virus Shedding in Semen: A Case Series

Background: Chikungunya is a viral disease that is transmitted by mosquitoes. It is characterized by an acute onset of fever and severe arthralgia. Methods: We describe six cases of acute and post-acute chikungunya in which viral RNA was detected in semen. Conclusions: The most prolonged detection period was 56 days after illness onset. We attempted to cultivate positive semen samples, but virus isolation was unsuccessful in all cases

Armazenamento de melão amarelo, híbrido RX 20094, sob temperatura ambiente Yellow melon, RX 20094 hybrid, stored at room temperature

A vida útil pós-colheita do melão amarelo híbrido RX 20094 foi avaliada, estudando-se características visuais, físicas e físico-químicas dos frutos em pós-colheita em ensaio conduzido no laboratório da Escola Superior de Agricultura de Mossoró. Utilizou-se delineamento inteiramente casualizado com seis tratamentos, referentes aos períodos de armazenamento (0; 5; 10; 15; 20; e 25 dias) e 5 repetições, com dois frutos por parcela. Os frutos foram mantidos à temperatura de 30±1ºC e UR de 50±5%. Foram feitas avaliações em intervalos de 5 dias, avaliando-se a firmeza da polpa, perda de massa, aparências externa e interna e conteúdo de sólidos solúveis (SS). Observou-se efeito do tempo de armazenamento para todas as características avaliadas. A firmeza da polpa e as aparências externa e interna foram os principais fatores responsáveis pela perda de qualidade do melão. A vida útil do híbrido RX-20094 foi estimada em até no mínimo 25 dias de armazenamento sob temperatura ambiente.<br>The postharvest shelflife of yellow melon RX20094 hybrid was obtained through visual, physical, and physical-chemical evaluations in two experiments carried out at Escola Superior de Agricultura de Mossoró, Rio Grande do Norte State, Brasil. A completely randomized design with six treatments, consisting of storage days (0; 5; 10; 15; 20; and 25) and five replications, with 2 fruits per plot, was utilized. The fruits were kept at the temperature of 30±1ºC and 50±5% of relative humidity. Evaluations were made at five-day intervals with the determination of flesh firmness, weight loss, external and internal appearances and soluble solids content (SS). The storage days had effect on all characteristics. Yellow melons RX20094 hybrid maintained good quality characteristics for up to a storage period of 25 days at room temperature

As experiências de consumo na perspectiva da teoria da cultura do consumo: identificando possíveis interlocuções e propondo uma agenda de pesquisa

O tema norteador deste trabalho refere-se à constatação de que a pesquisa do consumidor precisa incorporar em suas análises elementos de noção experiencial do consumo e a percepção de que pesquisadores brasileiros têm ignorado um conjunto de temas que leve a uma melhor compreensão dos atos de consumo, de seus sujeitos e contextos culturais. Os principais objetivos aqui estão em discutir uma proposta de interlocução entre a literatura da cultura do consumo e as discussões advindas da noção experiencial do consumo, por meio de temas emergentes de pesquisa; propor alternativas metodológicas para o entendimento das experiências de consumo na perspectiva da cultura de consumo. Conclui-se que não somente é possível, mas altamente desejável, estabelecer ricas e profícuas discussões sobre as possibilidades de interlocução entre esses campos, propondo novas epistemologias e metodologias que contribuam para uma perspectiva que abranja o entendimento do consumo em uma abordagem experiencial e como fenômeno cultural

Risk of adverse outcomes in offspring with RT-PCR confirmed prenatal Zika virus exposure: an individual participant data meta-analysis of 13 cohorts in the Zika Brazilian Cohorts ConsortiumResearch in context

Summary: Background: Knowledge regarding the risks associated with Zika virus (ZIKV) infections in pregnancy has relied on individual studies with relatively small sample sizes and variable risk estimates of adverse outcomes, or on surveillance or routinely collected data. Using data from the Zika Brazilian Cohorts Consortium, this study aims, to estimate the risk of adverse outcomes among offspring of women with RT-PCR-confirmed ZIKV infection during pregnancy and to explore heterogeneity between studies. Methods: We performed an individual participant data meta-analysis of the offspring of 1548 pregnant women from 13 studies, using one and two-stage meta-analyses to estimate the absolute risks. Findings: Of the 1548 ZIKV-exposed pregnancies, the risk of miscarriage was 0.9%, while the risk of stillbirth was 0.3%. Among the pregnancies with liveborn children, the risk of prematurity was 10,5%, the risk of low birth weight was 7.7, and the risk of small for gestational age (SGA) was 16.2%. For other abnormalities, the absolute risks were: 2.6% for microcephaly at birth or first evaluation, 4.0% for microcephaly at any time during follow-up, 7.9% for neuroimaging abnormalities, 18.7% for functional neurological abnormalities, 4.0% for ophthalmic abnormalities, 6.4% for auditory abnormalities, 0.6% for arthrogryposis, and 1.5% for dysphagia. This risk was similar in all sites studied and in different socioeconomic conditions, indicating that there are not likely to be other factors modifying this association. Interpretation: This study based on prospectively collected data generates the most robust evidence to date on the risks of congenital ZIKV infections over the early life course. Overall, approximately one-third of liveborn children with prenatal ZIKV exposure presented with at least one abnormality compatible with congenital infection, while the risk to present with at least two abnormalities in combination was less than 1.0%. Funding: National Council for Scientific and Technological Development - Brazil (Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq); Wellcome Trust and the United Kingdom's Department for International Development; European Union's Horizon 2020 research and innovation program; Medical Research Council on behalf of the Newton Fund and Wellcome Trust; National Institutes of Health/National Institute of Allergy and Infectious Diseases; Foundation Christophe et Rodolphe Mérieux; Coordination for the improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Capes); Ministry of Health of Brazil; Brazilian Department of Science and Technology; Foundation of Research Support of the State of São Paulo (Fundação de Amparo à Pesquisa do Estado de São Paulo – FAPESP); Foundation of Research Support of the State of Rio de Janeiro (Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro – FAPERJ); Foundation of Support for Research and Scientific and Technological Development of Maranhão; Evandro Chagas Institute/Brazilian Ministry of Health (Instituto Evandro Chagas/Ministério da Saúde); Foundation of Research Support of the State of Goiás (Fundação de Amparo à Pesquisa do Estado de Goiás – FAPEG); Foundation of Research Support of the State of Rio Grande do Sul (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul – FAPERGS); Foundation to Support Teaching, Research and Assistance at Hospital das Clínicas, Faculty of Medicine of Ribeirão Preto (Fundação de Apoio ao Ensino, Pesquisa e Assistência do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto); São Paulo State Department of Health (Secretaria de Saúde do Estado de São Paulo); Support Foundation of Pernambuco Science and Technology (Fundação de Amparo à Ciência e Tecnologia de Pernambuco – FACEPE)