Mineral nutrients in soil and pea plants after exposition to TiO2 nanoparticles through a biosolid-amended soil

In addition to the benefits derived from nanotechnology, there is also concern about the potential risks of engineered nanoparticles (ENPs) when released into the environment. Their possible accumulation and effects in agricultural soils and Nanonnovation 2018 Conference & Exibition crops are closely linked to food and agriculture safety. Particular attention has been focused on the reuse of biosolids from wastewater treatment plants that are considered a cost-effective practice for the improvement of nutrients and organic matter in agricultural soils and, but also a sink of contaminants such as nanoparticles (NPs). TiO2NPs have a global production of about 10.000 tons/year and it are among the most extensively used ENPs. Moreover, dissimilar or inconclusive results have been reported concerning the impact of TiO2 NPs on the soil-crop system, thus more information regarding their behavior are necessary. This study aimed to evaluate the potential effects of TiO2 NPs (anatase and rutile) and larger particles (bulk) on the availability of soil nutrients and on the nutritional status of Pisum sativum plants, simulating low (80 mg/kg) and high load of TiO2 (800 mg/kg ) in a biosolid-amended soil. Treated soils were analyzed for N, P, K, Ca, Mg, Mn, Fe, Cu, Zn, soil microbial community, and plants grown in laboratory for 30d were analyzed for growth, pigments and mineral nutrition. Results showed that the treatment with TiO2 at macro- and nano-scale significantly reduced the availability of Mn, Fe and P in soils, this last more evident for the NPs treatments. Indeed, the soil bacterial diversity was reduced when the mixture of anatase and rutile were spiked in the biosolid-amended soil at high concentration. Moreover, the pea plants from treated soils showed an imbalance in the mineral nutrition, with reduction in the plant tissues of Mn and K and increase of N. This study pose a reflection on the use of biosolid, which could act as a vehicle for the spread and accumulation of ENPs in agro-ecosystems

Two further patients with Warsaw breakage syndrome. Is a mild phenotype possible?

Background: Warsaw Breakage Syndrome (WABS) is an ultra rare cohesinopathy caused by biallelic mutation of DDX11 gene. It is clinically characterized by pre and postnatal growth delay, microcephaly, hearing loss with cochlear hypoplasia, skin color abnormalities, and dysmorphisms. Methods: Mutational screening and functional analyses (protein expression and 3D-modeling) were performed in order to investigate the presence and pathogenicity of DDX11 variant identified in our patients. Results: We report the clinical history of two sisters affected by WABS with a pathological mytomicin C test carrying compound heterozygous mutations (c.2507T > C / c.907_920del) of the DDX11 gene. The pathogenicity of this variant was confirmed in the light of a bioinformatic study and protein three-dimensional modeling, as well as expression analysis. Conclusion: These findings further extend the clinical and molecular knowledge about the WABS showing a possible mild phenotype without major malformations or intellectual disability

Manual de produção de uvas viníferas de alta qualidade.

bitstream/item/129038/1/MANUAL-de-PRODUCAO-de-UVAS-VINIFERAS-de-ALTA-QUALIDADE-2015-Revisado-em-31-08-15.pd

Normas técnicas da produção de uva para processamento - vinho e suco.

Neste capítulo, apresenta-se a versão atualizada das Normas Técnicas da Produção Integrada de Uva Para Processamento (PIUP), elaboradas dentro do Projeto da PIUP, pelos pesquisadores da Embrapa e com a participação efetiva do setor produtivo. No primeiro semestre do ano de 2010 foi obtida a primeira versão das Normas da PIUP, as quais foram apresentadas ao setor vitivinícola em encontro realizado nas dependências da Embrapa Uva e Vinho e, em seguida, colocadas na página da instituição para discussão e participação do setor.bitstream/item/152896/1/Manual-1-1-Capitulo-2.pd