Developing a site-conditions map for seismic hazard Assessment in Portugal

The evaluation of site effects on a broad scale is a critical issue for seismic hazard and risk assessment, land use planning and emergency planning. As characterization of site conditions based on the shear-wave velocity has become increasingly important, several methods have been proposed in the literature to estimate its average over the first thirty meters (Vs30) from more extensively available data. These methods include correlations with geologic-geographic defined units and topographic slope. In this paper we present the first steps towards the development of a site–conditions map for Portugal, based on a regional database of shear-wave velocity data, together with geological, geographic, and lithological information. We computed Vs30 for each database site and classified it according to the corresponding geological-lithological information using maps at the smallest scale available (usually 1:50000). We evaluated the consistency of Vs30 values within generalized-geological classes, and assessed the performance of expedient methodologies proposed in the literature

Synaptic transmission between pairs of retinal amacrine cells in culture

We have examined synaptic transmission between isolated pairs of chick GABAergic amacrine cells, maintained in sparse culture and identified by their binding of an amacrine cell-selective antibody. Using the perforated- patch method to whole-cell clamp both cells of a pair, postsynaptic currents were examined for step depolarizations of the \u27presynaptic\u27 cell. Synaptic transmission, frequently reciprocal, was calcium dependent and reversibly blocked by bicuculline. Postsynaptic currents, excluding those due to ohmic electrical coupling, were elicited only for presynaptic voltage steps positive to about -40 mV and were always very noisy, suggesting that they were summed from relatively small numbers of quanta. Postsynaptic currents continued well after the termination of the 100 msec presynaptic voltage step when the step was to -10 mV, or positive to this value. This result is interpreted to imply that presynaptic calcium concentration remains elevated after the membrane is returned to its holding potential. When presynaptic voltages were kept low or else presynaptic voltage was uncontrolled, spontaneous quantal events mediated by GABA(A) receptors could often be seen. Quanta rose quickly (less than 4 msec) and decayed with a mean time constant of 19.3 msec. The amplitude distributions of quantal currents were positively skewed, sometimes showing rare quanta of exceptionally large amplitude. Peak conductance per quantum was about 300 pS, corresponding to the simultaneous opening of only 17 GABA(A) channels and corresponding to a net flux of only 32 x 103 Cl- ions per millivolt of driving force. Estimates of the maximum sustained release rate at individual release sites suggest an upper bound of between 19 and 42 quanta per second

Complementary action of chemical and electrical synapses to perception

Acknowledgements This study was possible by partial financial support from the following agencies: Fundação Araucária, EPSRC-EP/I032606/1, CNPq No. 441553/2014-1, CAPES No. 17656-12-5 and Science Without Borders Program— Process Nos. 17656125, 99999.010583/2013-00 and 245377/2012-3.Peer reviewedPostprin

Electrogenic Na-Ca exchange clears Ca\u3csup\u3e2+\u3c/sup\u3e loads from retinal amacrine cells in culture

Calcium influx into cultured retinal amacrine cells is followed by a small, slow, inward current that we show here results from the operation of electrogenic Na-Ca exchange. The activity of the exchanger is shown to correlate with the magnitude of the Ca2+ load and to depend on both the Ca2+ and Na+ gradients. Li+ is unable to substitute for Na+ and in the absence of Na+, slow tail currents are almost entirely suppressed. A rapid change in [K+](o) does not affect the activity of the exchanger, suggesting that only Na+ and Ca2+ are transported. The ratio of charge entering as Ca2+ current to the charge entering as exchange current is highly variable between cells. We suggest that variability results from a variable fraction of Ca2+ load, we estimate typically 40%, being removed by a process other than Na-Ca exchange. This process is likely to involve internal buffering or sequestration since inhibition of the plasmalemmal Ca2+ATPase does not increase the fraction of Ca2+ expelled by the exchanger. Ca2+ loading performed in the absence of Na+(o) generates smaller exchange charge the longer the delay in returning Na+(o) to the neuron. About 30% of exchange charge is lost for a delay of 1 sec

Generating dynamic higher-order Markov models in web usage mining

Markov models have been widely used for modelling users’ web navigation behaviour. In previous work we have presented a dynamic clustering-based Markov model that accurately represents second-order transition probabilities given by a collection of navigation sessions. Herein, we propose a generalisation of the method that takes into account higher-order conditional probabilities. The method makes use of the state cloning concept together with a clustering technique to separate the navigation paths that reveal differences in the conditional probabilities. We report on experiments conducted with three real world data sets. The results show that some pages require a long history to understand the users choice of link, while others require only a short history. We also show that the number of additional states induced by the method can be controlled through a probability threshold parameter

Probing the ejecta of evolved massive stars in transition: A VLT/SINFONI K-band survey

Massive evolved stars in transition phases, such as Luminous Blue Variables (LBVs), B[e] Supergiants (B[e]SGs), and Yellow Hypergiants (YHGs), are not well understood, and yet crucial steps in determining accurate stellar and galactic evolution models. The circumstellar environments of these stars reveal their mass-loss history, identifying clues to both their individual evolutionary status and the connection between objects of different phases. Here we present a survey of 25 such evolved massive stars (16 B[e]SGs, 6 LBVs, 2 YHGs, and 1 Peculiar Oe star), observed in the K-band with the Spectrograph for INtegral Field Observation in the Near-Infrared (SINFONI; R = 4500) on the ESO VLT UT4 8 m telescope. The sample can be split into two categories based on spectral morphology: one group includes all of the B[e]SGs, the Peculiar Oe star, and two of the LBVs, while the other includes the YHGs and the rest of the LBVs. The difference in LBV spectral appearance is due to some objects being in a quiescent phase and some objects being in an active or outburst phase. CO emission features are found in 13 of our targets, with first time detections for MWC 137, LHA 120-S 35, and LHA 115-S 65. From model fits to the CO band heads, the emitting regions appear to be detached from the stellar surface. Each star with ^12CO features also shows ^13CO emission, signaling an evolved nature. Based on the level of ^13C enrichment, we conclude that many of the B[e]SGs are likely in a pre-Red Supergiant phase of their evolution. There appears to be a lower luminosity limit of log L/L_solar = 5.0 below which CO is not detected. The lack of CO features in several high luminosity B[e]SGs and variability in others suggests that they may in fact be LBV candidates, strengthening the connection between these two very similar transition phases.Comment: 20 pages, 13 figures, 7 tables. Accepted for publication in A&

Alterations in brain connectivity due to plasticity and synaptic delay

Brain plasticity refers to brain's ability to change neuronal connections, as a result of environmental stimuli, new experiences, or damage. In this work, we study the effects of the synaptic delay on both the coupling strengths and synchronisation in a neuronal network with synaptic plasticity. We build a network of Hodgkin-Huxley neurons, where the plasticity is given by the Hebbian rules. We verify that without time delay the excitatory synapses became stronger from the high frequency to low frequency neurons and the inhibitory synapses increases in the opposite way, when the delay is increased the network presents a non-trivial topology. Regarding the synchronisation, only for small values of the synaptic delay this phenomenon is observed