20 research outputs found
PHYTOCHEMICAL STUDY AND IN VITRO ANTIOXIDANT ACTIVITIES OF HAMMADA SCOPARIA EXTRACTS FROM SOUTHEASTERN ALGERIA
Objective: This study was carried out to determine the phytochemical constituents and to evaluate the antioxidant potential of the aerial part extracts of Hammada scoparia (Pomel) Iljin to validate the medicinal potential of this Algerian plant species.Methods: Crude extracts were prepared by cold maceration with absolute methanol and distilled water. Preliminary phytochemical screening is carried out to detect the presence of the major secondary metabolites using qualitative characterization methods. Quantitative estimation of total phenols, total flavonoids, flavanols, flavonols, and condensed tannins contents is performed using gallic acid, rutin, catechin, and quercetin as standards. In vitro antioxidant activity was evaluated by the free radical scavenging activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity by scavenging of 2,2ĂąâŹÂČ-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation assay, and the ferric reducing power assay (ferric reducing antioxidant power).Results: Phytochemical screening showed the presence of alkaloids, flavonoids, tannins, terpenes and sterols, saponins, and anthocyanins. Total phenols were present more in aqueous crude extract (ACE) with 336.756ñ0.855 mg gallic acid equivalent/g DM. Total flavonoids and flavonols were more abundant in methanolic crude extract (MCE) than ACE. However, condensed tannins and flavanols were present less in MCE with only 0.958ñ0.052 and 4.547ñ0.055 mg CE/g DM, respectively. The ACE of this plant species had greater antioxidant activity than the other extract by DPPH and ABTS assays (35.823ñ0.129 and 51.323ñ0.394 mg trolox equivalent/g DM). The better ferric reducing power (2060.535ñ2.566 mM Fe+2/g DM) was also recorded with the same extract.Conclusion: ACE of aerial part of H. scoparia (Pomel) Iljin showed a high amount of secondary metabolites. The obtained results confirmed that the extracting solvent influenced the antioxidant property estimations of this plant. Hence, the ACE can be further exploited further for in vitro and in vivo research work
ActivitĂ© antibactĂ©rienne des flavonoides dâune plante mĂ©dicinale spontanĂ©e Marrubium vulgare L. de la rĂ©gion dâEl Tarf (Nord-Est AlgĂ©rien)
La phytothĂ©rapie, proposant des remĂšdes naturels, est bien acceptĂ©e par lâorganisme. Elle connaĂźt actuellement un renouveau exceptionnel en occident du fait des effets secondaires induits par les mĂ©dicaments inquiĂ©tant les utilisateurs qui font alors appel Ă une mĂ©decine plus douce. Aussi, notre intĂ©rĂȘt sâest portĂ© sur Marrubium vulgare L ou Marrube blanc, espĂšce vĂ©gĂ©tale utilisĂ©e en phytothĂ©rapie, afin de vĂ©rifier lâefficacitĂ© de cette plante sur le plan antiseptique et notamment lâactivitĂ© antibactĂ©rienne de ses flavonoĂŻdes. Lâextraction des flavonoĂŻdes a donnĂ© un rendement assez important Ă©gal Ă 5.9 %. Les composĂ©s flavonoiques isolĂ©s ont Ă©tĂ© sĂ©parĂ©s par CCM et leur activitĂ© antibactĂ©rienne vis-Ă -vis de six souches bactĂ©riennes responsables de certaines maladies infectieuses a Ă©tĂ© dĂ©terminĂ©e in vitro. Des tests de comparaison avec un antibiotique, la Rifampicine ont Ă©tĂ© Ă©galement inclus dans les essais. Les rĂ©sultats obtenus rĂ©vĂšlent que lâextrait isolĂ© est formĂ© de deux composĂ©s ayant des rapports frontaux plus ou moins rapprochĂ©s. Les tests antibactĂ©riens montrent que lâinhibition de la croissance des souches varie en fonction de la concentration de lâextrait et du milieu de culture utilisĂ©. Un effet antibactĂ©rien important a Ă©tĂ© observĂ© vis-Ă -vis de Pseudomonas aeruginosa ATCC 27853 et de Staphylococcus aureus.Mots clĂ©s : ActivitĂ© antibactĂ©rienne - FlavonoĂŻdes - Marrubium vulgare L. - Souches multirĂ©sistantes
Etude de lâactivitĂ© antibactĂ©rienne des huiles essentielles de la Sauge officinale : Salvia officinalis L. sur quelques entĂ©robactĂ©ries pathogĂšnes
LâĂ©mergence de microorganismes pathogĂšnes, pose actuellement un problĂšme de santĂ© publique particuliĂšrement prĂ©occupant. En effet, la rĂ©sistance des germes aux antibiotiques rend quelques fois le traitement thĂ©rapeutique inefficace et impose la recherche de nouveaux agents antimicrobiens. Le recours aux plantes mĂ©dicinales constitue alors une des plus intĂ©ressantes pistes Ă explorer ; câest dans cette perspective que nous nous sommes intĂ©ressĂ©s Ă lâĂ©tude de lâactivitĂ© antibactĂ©rienne des feuilles de la Sauge officinale (Salvia officinalis L.) de la rĂ©gion du nord-est AlgĂ©rien. Dans cette Ă©tude, nous avons procĂ©dĂ© Ă lâextraction et lâidentification des huiles essentielles ainsi que lâĂ©valuation de leur pouvoir antibactĂ©rien vis-Ă -vis de quelques entĂ©robactĂ©ries pathogĂšnes. Les rĂ©sultats obtenus ont dĂ©montrĂ© que lâhuile essentielle isolĂ©e est formĂ©e de dix-neuf (19) composĂ©s terpĂ©niques avec une prĂ©dominance de la thujone (α- et ÎČ-thujone). Dâune maniĂšre gĂ©nĂ©rale, les huiles essentielles obtenues ont un pouvoir antibactĂ©rien intĂ©ressant sur les germes Ă©tudiĂ©s. Les zones dâinhibition enregistrĂ©es dĂ©passent le plus souvent celles provoquĂ©es par lâantibiotique utilisĂ©. Ces rĂ©sultats sont prometteurs et apportent une validation scientifique quant Ă lâusage massif de cette espĂšce. Ainsi lâeffet des substances naturelles extraites des plantes mĂ©dicinales pourraient bien rivaliser celui des antibiotiques.Mots clĂ©s : ActivitĂ© antibactĂ©rienne â Huiles essentielles â Salvia officinalis L. âEntĂ©robactĂ©ries â Nord-Est algĂ©rien
Phytochemical screening and biological activity of essential oils and flavonoĂŻds of aromatic plant Salvia officinalis L. in north-eastern Algeria
Evaluation of Algerian spontaneous species: phytochemical and biological study of aromatic and medicinal plant Marrubium vulgare
Synthesis and Characterization of Monolithic Carbon Aerogel Nanocomposites Containing Double-Walled Carbon Nanotubes
Isolamento e seleção de fungos para biorremediação a partir de solo contaminado com herbicidas triazĂnicos Isolation and screening of fungi to bioremediation from triazine herbicide contaminated soil
A biorremediação Ă© uma tecnologia que utiliza o metabolismo de microrganismos para eliminação ou redução, a nĂveis aceitĂĄveis, de poluentes presentes no ambiente. Os herbicidas triazĂnicos sĂŁo usados intensivamente no controle de ervas daninhas, principalmente na cultura de milho. Objetivou-se, neste trabalho, isolar fungos filamentosos de solos contaminados com herbicidas triazĂnicos (atrazine e simazine) e selecionar os microrganismos isolados quanto Ă capacidade de crescimento em meio adicionado de atrazine. Os microrganismos foram isolados, cultivados em meio Ăgar-Batata-Dextrose (BDA) acidificado com ĂĄcido tartĂĄrico 10%, adicionado de 50 mg.Kg-1 de atrazine e incubados por 5 dias a 25ÂșC. Foi realizada a medida diĂĄria do crescimento fĂșngico e calculada a velocidade de crescimento radial atravĂ©s de regressĂŁo linear dos raios das colĂŽnias utilizando-se a equação r(t) = a + VCR .t (r:raio; t: tempo; VCR: velocidade de crescimento radial). Os resultados de VCR foram analisados atravĂ©s de Anova simples e do teste de Tukey, para comparação de mĂ©dias. Foram isolados 15 fungos, pertencentes aos gĂȘneros Aspergillus, Penicillium e Trichoderma. As maiores VCRs foram obtidas com fungos Aspergillus (A1) e Penicillium (AS1), isolados de solo contaminado com atrazine e atrazine adicionado de simazine, respectivamente, que apresentaram VCRs de 1,57 mm.d-1 e 1,28 mm.d-1. O crescimento dos fungos em meio contaminado com a atrazine indica a possibilidade de utilização desses fungos em estudos de biorremediação de solos contaminados com herbicidas triazĂnicos.<br>Bioremediation is a technology that uses microrganism metabolism to quickly eliminate or reduce pollutants to acceptable levels into the environment. The triazine herbicides are intensively used to control harmful grass in the culture of maize. The aim of this work was to isolate filamentous fungi from soil contaminated with triazine herbicides and screening these fungi due to their ability of growth in a medium added by atrazine. The fungi were isolated, cultivated in potato-dextrose-agar plus 50 mg.Kg-1 of atrazine and incubated for 5 days at 25ÂșC. The measure of the rays of the colonies was carried out daily and the radial growth rate (RGR) through linear regression of colonies rays using the equation: r(t) = a + RGR .t (r:ray; t: time; RGR: radial growth rate). The RGR results were analyzed through analysis of variance and Tukey test for comparison of averages. Fifteen filamentous fungi from genus Aspergillus, Penicillium and Trichoderma were isolated. The highest RGRs were obtained with the fungi Aspergillus and Penicillium, when isolated from contaminated soil with atrazine and atrazine + simazine, respectively, showing RGR of 1.57 mm.d-1 and 1.28 mm.d-1. The growth of these fungi in atrazine contaminated meas indicates a possible of use of them in bioremediation experiments with contaminated soil containing triazine herbicides