Synthesis and biological evaluation of anti-Toxoplasma gondii activity of a novel scaffold of thiazolidinone derivatives

We designed and synthesised novel N-substituted 1,3-thiazolidin-4-one derivatives for the evaluation of their anti-Toxoplasma gondii efficacy. This scaffold was functionalised both at the N1-hydrazine portion with three structurally different moieties and at the lactam nitrogen with substituted benzyl groups selected on the basis of our previous structure-activity relationships studies. Using three different assay methods, the compounds were assessed in vitro to determine both the levels of efficacy against the tachyzoites of T. gondii (IC50 = 5-148 μM), as well as any evidence of cytotoxicity towards human host cells (TD50 = 68 to ≥320 μM). Results revealed that ferrocene-based thiazolidinones can possess potent anti-tachyzoite activity (TI =2-64)

Concursos públicos : aquisição local de equipamentos de proteção individual no contexto de descentralização e elevada desigualdade socioeconômica

Published: 15 December 2023Published in two languages: English (original version) and Portuguese (translation)Under the influence of fiscal federalism and government decentralization theories, a significant part of health systems around the world confronted the COVID-19 pandemic after being shaped or re-shaped by processes of devolution from central to local governments. Procurement of key supplies is one of the components that operate in a decentralized manner, forcing local governments to compete against each other. This was the origin of what has been called the “bidding wars” between subnational governments at the beginning of the pandemic response. These wars led to centralization policies in the United States, the United Kingdom, and the European Union. Yet, less is known about cases from the Global South. By analyzing the procurement of Personal Protective Equipment (PPE) in the 320 Chilean municipalities in charge of primary health, this research provides evidence of the impacts of horizontal government competition on the ability to procure key supplies. In Chile, during the 2020 response to the pandemic, richer municipalities were able to procure more face masks per population, while economies of scale rewarded bigger purchases with lower prices. The authors support the theoretical notion of “concurrency” as a concept that adds nuances to the centralization-decentralization debate. In Chile, for instance, while testing and tracking required decentralization, PPE purchases could have probably benefited from centralization in order to avoid reproducing territorial inequalities.Sob a influência do federalismo fiscal e das teorias de descentralização governamental, uma parte significativa dos sistemas de saúde em todo o mundo enfrentou a pandemia da COVID-19 depois de terem sido moldados ou remodelados por processos de transferência dos governos centrais para os governos locais. A aquisição de suprimentos essenciais é um dos componentes que operam de forma descentralizada, forçando os governos locais a competir entre si. Esta foi a origem do que foi chamado de “guerras de licitações” entre governos subnacionais no início da resposta à pandemia. Essas guerras levaram a políticas de centralização nos Estados Unidos, no Reino Unido e na União Europeia. No entanto, sabe-se menos sobre os casos do Sul Global. Ao analisar a aquisição de Equipamentos de Proteção Individual (EPI) nos 320 municípios chilenos responsáveis pela saúde primária, esta pesquisa fornece evidências dos impactos da competição governamental horizontal na capacidade de adquirir suprimentos essenciais. No Chile, durante a resposta à pandemia de 2020, os municípios mais ricos conseguiram adquirir mais máscaras faciais por população, enquanto as economias de escala recompensaram compras maiores com preços mais baixos. Os autores apoiam a noção teórica de simultaneidade como um conceito que acrescenta nuances ao debate centralização-descentralização. No Chile, por exemplo, embora os testes e o acompanhamento exigissem a descentralização, as compras de EPI poderiam provavelmente ter beneficiado da centralização, a fim de evitar a reprodução de desigualdades territoriais.Funding support from Margarita Salas, the Spanish Ministry of Universities, and the European Union, specifically through the European Union-Next Generation EU program

Dual TEVT education in Chile: Why do companies train students?

This paper analyses the costs and benefits of dual training programmes for companies in Chile. Using an online survey, we estimate that Chilean companies that participate incur net costs of US$3,200 per student per year in training; however, most companies declared to be satisfied with dual training. We simulate two scenarios under which dual training is cost effective. The first scenario simulates a subsidy equal to the net costs for each student companies receive. The second scenario assumes an increase of 25% in the productivity of apprentices, which reduces the students time in practicing and increases the productivity of the workers in charge, decreasing their time dedicated to train the students by 50%. Public policies to promote early adjustment such that students arrive at companies better prepared and can allocate more time to productive tasks should be considered to decrease the net cost of training

Employer Learning, Statistical Discrimination and University Prestige

This paper investigates whether firms use university prestige to statistically discriminate among college graduates. The test is based on the employer learning literature which suggests that if firms use a characteristic for statistical discrimination, this variable should become less important for earnings determination as a worker gains labor market experience. In this framework, we use a regression discontinuity design to estimate a 19 % wage premium for recent graduates of two of the most selective universities in Chile. However, we find that this premium decreases by 3 percentage points per year of labor market experience. These results suggest that employers use college selectivity as a signal of workers ’ quality when they leave school. However, as workers reveal their productivity throughout their careers, they become rewarded based on their true quality rather than the prestige of their college

Synthesis and Evaluation of 4-Acyl-2- Thiazolylhydrazone Derivatives for anti-Toxoplasma Efficacy in Vitro

A new series of 4-acyl-2-thiazolylhydrazone derivatives was synthesized and screened for its in vitro activity against Toxoplasma gondii. We evaluated parasite growth inhibition and cytotoxicity, inhibition of replication, and inhibition of parasite invasion of host cells. The biological results indicated that some substances had an antiproliferative effect against intracellular T. gondii tachyzoites cultivated in vitro

Synthesis and biological evaluation of anti-<i>Toxoplasma gondii</i> activity of a novel scaffold of thiazolidinone derivatives

<p>We designed and synthesised novel <i>N</i>-substituted 1,3-thiazolidin-4-one derivatives for the evaluation of their anti-<i>Toxoplasma gondii</i> efficacy. This scaffold was functionalised both at the N1-hydrazine portion with three structurally different moieties and at the lactam nitrogen with substituted benzyl groups selected on the basis of our previous structure-activity relationships studies. Using three different assay methods, the compounds were assessed <i>in vitro</i> to determine both the levels of efficacy against the tachyzoites of <i>T. gondii</i> (IC₅₀ = 5–148 μM), as well as any evidence of cytotoxicity towards human host cells (TD₅₀ = 68 to ≥320 μM). Results revealed that ferrocene-based thiazolidinones can possess potent anti-tachyzoite activity (TI =2–64).</p