Efficient CO2-Reducing Activity of NAD-Dependent Formate Dehydrogenase from Thiobacillus sp KNK65MA for Formate Production from CO2 Gas

NAD-dependent formate dehydrogenase (FDH) from Candida boidinii (CbFDH) has been widely used in various CO2 reduction systems but its practical applications are often impeded due to low CO2-reducing activity. In this study, we demonstrated superior CO2-reducing properties of FDH from Thiobacillus sp. KNK65MA (TsFDH) for production of formate from CO2 gas. To discover more efficient CO2-reducing FDHs than a reference enzyme e. CbFDH, five FDHs were selected with biochemical properties and then, their CO2-reducing activities were evaluated. All FDHs including CbFDH showed better CO2-reducing activities at acidic pHs than at neutral pHs and four FDHs were more active than CbFDH in the CO2 reduction reaction. In particular, the FDH from Thiobacillus sp. KNK65IVIA (TsFDH) exhibited the highest CO2-reducing activity and had a dramatic preference for the reduction reaction, i.e., a 84.2-fold higher ratio of CO2 reduction to formate oxidation in catalytic efficiency (k(cat)/K-B) compared to CbFDH. Formate was produced from CO2 gas using TsFDH and CbFDH, and TsFDH showed a 5.8-fold higher formate production rate than CbFDH. A sequence and structural comparison showed that FDHs with relatively high CO2-reducing activities had elongated N- and C-terminal loops. The experimental results demonstrate that TsFDH can be an alternative to CbFDH as a biocatalyst in CO2 reduction systemsope

Botany, chemistry, and pharmaceutical significance of Sida cordifolia: a traditional medicinal plant

Sida cordifolia Linn. belonging to the family, Malvaceae has been widely employed in traditional medications in many parts of the world including India, Brazil, and other Asian and African countries. The plant is extensively used in the Ayurvedic medicine preparation. There are more than 200 plant species within the genus Sida, which are distributed predominantly in the tropical regions. The correct taxonomic identification is a major concern due to the fact that S. cordifolia looks morphologically similar with its related species. It possesses activity against various human ailments, including cancer, asthma, cough, diarrhea, malaria, gonorrhea, tuberculosis, obesity, ulcer, Parkinson’s disease, urinary infections, and many others. The medical importance of this plant is mainly correlated to the occurrence of diverse biologically active phytochemical compounds such as alkaloids, flavonoids, and steroids. The major compounds include β-phenylamines, 2-carboxylated tryptamines, quinazoline, quinoline, indole, ephedrine, vasicinone, 5-3-isoprenyl flavone, 5,7-dihydroxy-3-isoprenyl flavone, and 6-(isoprenyl)- 3-methoxy- 8-C-β-D-glucosyl-kaempferol 3-O-β-D-glucosyl[1–4]-α-D-glucoside. The literature survey reveals that most of the pharmacological investigations on S. cordifolia are limited to crude plant extracts and few isolated pure compounds. Therefore, there is a need to evaluate many other unexplored bioactive phytoconstituents with evidences so as to justify the traditional usages of S. cordifolia. Furthermore, detailed studies on the action of mechanisms of these isolated compounds supported by clinical research are necessary for validating their application in contemporary medicines. The aim of the present chapter is to provide a detailed information on the ethnobotanical, phytochemical, and pharmacological aspects of S. cordifolia

Revisiting probabilistic seismic hazard analysis of Gujarat: an assessment of Indian design spectra

Seismic hazard assessment is the key tool for rational planning, safety and design of infrastructures in seismically vulnerable regions. Gujarat is the only state in peninsular India with the maximum seismic hazard of large shallow earthquakes originating from intra-plate seismicity. Probabilistic seismic hazard assessment (PSHA) of Gujarat is carried out in this paper. Three seismogenic sources, namely Kutch, Saurashtra and Mainland Gujarat, are considered, and seismicity parameters are estimated separately for each region taking into account the completeness of the available earthquake data. Peak ground acceleration (PGA) of the horizontal component and spectral acceleration at specific periods are considered as the intensity measures. Ground motion predictive equation chosen was reported to be based on simulated ground motions and verified against the strong motion records in the study region. Results are reported for the 17 major cities at the bedrock and also for the soil sites. Apart from hazard curves, 2475 and 475 years of return periods are considered for the PGA and uniform hazard spectra (UHS). The results are compared with the present recommendations of Indian Standards. Key observations include (1) Indian Standards underpredict PGA in the entire Gujarat when the soil sites are considered and in a few cities even at the bedrock; (2) amplification of PGA (or short period hazard) on account of soil sites should be included in the Indian Standard, which is currently absent; (3) shape of the UHS indicates that a separate amplification is required at the hyperbolic portion; and (4) ratio of 2475–475 years of PGA, which is considered 2.0 in Indian Standard, should be reduced to 1.5. Time-dependent recurrence model is also included in this paper and compared with conventional PSHA. General observations include that (1) hazard may increase significantly on account of time dependency; (2) this also influences the disaggregation and in turn the selection of ground motions; and (3) time since last earthquake significantly influences the extent of the effect of time dependency.by Asim Bashir and Dhiman Bas