Ingress of Coolant Event simulation with TRACE code with accuracy evaluation and coupled DAKOTA Uncertainty Analysis

Among the Postulated Initiating Events in nuclear fusion plants, the Ingress of Coolant Event (ICE) in the Plasma Chamber is one of the main safety issues. In the present paper, the best estimate thermal-hydraulic system code TRACE, developed by USNRC, has been adopted to study the ICE, and it has been qualified based on experimental results obtained in the Integrated ICE facility at JAERI. A nodalization has been developed in the SNAP environment/architecture, using also the TRACE 3D Vessel component where multidimensional phenomena could occur. The accuracy of the code calculation has been assessed both from a qualitative and quantitative point of view. In addition, an Uncertainty Analysis (UA), with the probabilistic method to propagate the input uncertainties, has been performed to characterize the dispersion of the results. The analysis has been carried out with the DAKOTA toolkit coupled with TRACE code in the SNAP environment/architecture. Results show the adequacy of the 3D nodalization and the capability of the code to follow the transient evolution also at a very low pressure. Response correlations have been computed to characterize the correlation between the selected uncertain input parameters and the Plasma Chamber pressure

Antibacterial effects of two synthetic peptides against Enterococcus faecalis biofilms: A preliminary in vitro study

Aim: Current endodontic techniques are unable to fully eradicate intracanal bacteria. Thus, new agents that effectively eliminate endodontic pathogens are needed. The aim of this study was to assess the antibacterial properties of two synthetic peptides, namely KP and L18R, against planktonic cells and biofilms of the endodontic pathogen Enterococcus faecalis. Methodology: KP and L18R bactericidal activity against E. faecalis ATCC 29212 was evaluated by colony forming unit assays and the half maximal effective concentration (EC50) was calculated. The effect of peptides on E. faecalis biofilm formation onto polystyrene plates was also assessed by the crystal violet assay. Confocal laser scanning microscopy (CLSM) analysis was carried out to compare the effects of KP, L18R and a Ca(OH)2 saturated solution in an in vitro model of dental infection consisting in 2-day-old E. faecalis biofilms grown on hydroxyapatite disks. Results: Both KP and L18R showed strong bactericidal activity against planktonic E. faecalis. L18R proved to be 10-folds more ef fective than KP (KP and L18R EC50 values=4.520×10-6 M and 3.624×10-7 M, respectively). Peptides inhibited E. faecalis biofilm formation in a dose-dependent manner and L18R resulted more effectivethan KP. CLSM images showed that Ca(OH)2, KP and L18R remarkably impaired E. faecal is biof i lms pre -grown on hydroxyapatite. Conclusions: KP and L18R effectively inhibited E. faecalis, both in planktonic and biofilm form. L18R demonstrated a more potent antibacterial activity than KP. These preliminary results suggest that antimicrobial peptides may represent a promising new strategy for endodontic infection control

Activity of two antimicrobial peptides against Enterococcus faecalis in a model of biofilm-mediated endodontic infection

Enterococcus faecalis is a common cause of biofilm-associated opportunistic infections, which are often difficult to treat. The formation of E. faecalis biofilms on the dentinal walls of the root canal is frequently the cause of endodontic treatment failure and secondary apical periodontitis. In a preliminary work, two recognized antifungal peptides, KP and L18R, showed antibacterial activity against planktonic E. faecalis cells at micromolar concentrations. Moreover, L18R proved to reduce the biomass in the early stage of E. faecalis biofilm development on polystyrene plates, while a qualitative biofilm inhibition was demonstrated on hydroxyapatite disks by confocal laser scanning microscopy (CLSM). The aim of this study was to better characterize the effect of both peptides on E. faecalis biofilm. A reduction in metabolic activity after peptide treatment was detected by Al-amar Blue assay, while a remarkable impairment in the architecture of E. faecalis biofilms on hy-droxyapatite disks, along with a significant reduction in viable bacteria, was caused mostly by L18R, as assessed by CLSM and scanning electron microscopy. The lack of cytotoxicity of the investigated peptides against L929 murine fibroblasts was also determined. Obtained results suggest L18R as a promising candidate for the development of new strategies for endodontic infection control

Advanced 3D modeling versus Building Information Modeling: the case study of Palazzo Ettoreo in Sacile (Italy)

This paper presents an experience of 3D modeling starting from laser scanning data and following two alternative approaches: the first one, called \u201cAdvanced 3D modeling\u201d, based on an original meshing algorithm, while the second make use of Revit BIM software. The case study in Palace Ettoreo in Sacile (Pordenone, Italy), constructed in Renaissance Venetian style in the 16th century: it has a trapezoid plan and is developed on three floors, with the ground one endowing a portico on two fa\ue7ades. The palace has been surveyed by two terrestrial laser scanners: a Riegl Z420i for 5 external scans and a FARO Photon 120 for 53 internal scans; also a topographic surveying of 270 targets have been carried out. The final TLS cloud has 1,4 billions of points. The Advanced 3D modeling has produced a \u201csmart\u201d mesh, allowing also to model the elements with deformations (out of plumb, bulges and troughs). Moreover, this model drastically reduce the stored data: the whole palace is modeled by 111.496 polygons only. The modeling with Revit follows the classical flowchart where the principal architectonical elements are gradually composed: this HBIM process has required a strong manual work in exploiting the available parametric objects and/or in the definition of new objects. Comparing the two models with respect the points cloud, both have evidenced advantages and limitations: therefore, the best solution is a process involving their combination. At the beginning, the Advanced 3D modeling is performed onto the points cloud, so well exploiting the segmentation tools and the smart meshing of the surfaces preserving any geometrical irregularity. Such obtained model allows metrical and morphological evaluation on the various structural and architectonical elements. Afterwards, this very light model becomes the entry data for the modeling in BIM environment, where also the shape of irregular elements are so imported

First determination of the one-proton induced Non-Mesonic Weak Decay width of p-shell {\Lambda}-Hypernuclei

Previous studies of proton and neutron spectra from Non-Mesonic Weak Decay of eight Lambda-Hypernuclei (A = 5-16) have been revisited. New values of the ratio of the two-nucleon and the one-proton induced decay widths, Gamma_2N/Gamma_p, are obtained from single proton spectra, Gamma_2N/Gamma_p = 0.50 +/- 0.24, and from neutron and proton coincidence spectra, Gamma_2N/Gamma_p = 0.36 +/- 0.14stat +0.05sys -0.04sys , in full agreement with previously published ones. With these values, a method is developed to extract the one-proton induced decay width in units of the free Lambda decay width, Gamma_p/Gamma_Lambda, without resorting to Intra Nuclear Cascade models but by exploiting only experimental data, under the assumption of a linear dependence on A of the Final State Interaction contribution. This is the first systematic determination ever done and it agrees within the errors with recent theoretical calculations.Comment: 16 pages, 3 figures, 2 table

A georeferenced dataset of Italian occurrence records of the phylum Rotifera

We report a dataset of known and published occurrence records of Italian taxa from species (and subspecies) to family rank of the phylum Rotifera; we considered only Bdelloidea, Monogononta, and Seisonacea, and did not include Acanthocephala. The dataset in-cludes 15,525 records (12,015 of which with georeferenced coordinates) of 584 valid species and subspecies names and other taxa at family level, gathered from 332 published papers. The published literature spans the period from 1838 to 2022, with the lowest number of papers published during the first half of the twentieth century, followed by an increasing number of papers, from 20 to more than 60 in each decade. The Italian regions with the highest number of records and species are Emilia-Romagna, Lombardy, and Piedmont, whereas no records are known for Molise. The number of species known from each region mostly mirrors sampling efforts, measured as the number of publications per region. The dataset is available through the Open Science Framework (OSF), and all the georeferenced occurrence data have been uploaded to the Global Biodiversity Information Facility (GBIF). ial use only

Neonatal desensitisation for the study of regenerative medicine

Cell replacement is a therapeutic option for numerous diseases of the CNS. Current research has identified a number of potential human donor cell types, for which preclinical testing through xenotransplantation in animal models is imperative. Immune modulation is necessary to promote donor cell survival for sufficient time to assess safety and efficacy. Neonatal desensitization can promote survival of human donor cells in adult rat hosts with little impact on the health of the host and for substantially longer than conventional methods, and has subsequently been applied in a range of studies with variable outcomes. Reviewing these findings may provide insight into the method and its potential for use in preclinical studies in regenerative medicine

A georeferenced dataset of Italian occurrence records of the phylum Rotifera

We report a dataset of known and published occurrence records of Italian taxa from species (and subspecies) to family rank of the phylum Rotifera; we considered only Bdelloidea, Monogononta, and Seisonacea, and did not include Acanthocephala. The dataset includes 15,525 records (12,015 of which with georeferenced coordinates) of 584 valid species and subspecies names and other taxa at family level, gathered from 332 published papers. The published literature spans the period from 1838 to 2022, with the lowest number of papers published during the first half of the twentieth century, followed by an increasing number of papers, from 20 to more than 60 in each decade. The Italian regions with the highest number of records and species are Emilia-Romagna, Lombardy, and Piedmont, whereas no records are known for Molise. The number of species known from each region mostly mirrors sampling efforts, measured as the number of publications per region. The dataset is available through the Open Science Framework (OSF), and all the georeferenced occurrence data have been uploaded to the Global Biodiversity Information Facility (GBIF)

Σ−p\Sigma^- p emission rates in K−K^- absorptions at rest on 6^6Li, 7^7Li, 9^{9}Be, 13^{13}C and 16^{16}O

An experimental study of the $K^-_{stop}A\rightarrow \Sigma^- p A'$ reaction on $A=^6$Li, $^7$Li, $^9$Be, $^{13}$C and $^{16}$O $p$-shell nuclei is presented. The data were collected by the FINUDA spectrometer operating at the DA$\Phi$NE $\phi$-factory (LNF-INFN, Italy). Emission rates for the reaction in the mentioned nuclei are measured and compared with the few existing data. The spectra of several observables are discussed; indications of Quasi-Free absorptions by a $(np)$ pair embedded in the $A$ nucleus can be obtained from the study of the missing mass distributions.Comment: Version accepted by PR