Molecular detection of Rickettsia, Borrelia, and Babesia species in Ixodes ricinus sampled in northeastern, central, and insular areas of Italy.

The aim of the present study was to provide insight into the diversity of tick-borne pathogens circulating in Italy, carried/transmitted by Ixodes ricinus, one of the most abundant tick species in the country. A total of 447 specimens sampled in five areas of northeastern, central and insular Italy were analysed by polymerase chain reaction and sequencing for the presence of rickettsiae, borreliae and babesiae. Several rickettsial species of the spotted fever group of zoonotic concern and other zoonotic pathogens were found, such as Borrelia burgdorferi s.s., Borrelia afzelii, Borrelia garinii, and Babesia venatorum. These findings confirm a wide distribution of tick-borne bacterial and protozoan species in Italy, and highlight the sanitary importance of I. ricinus, often recorded as feeding on humans

Obtenção de biomassa microalgal tolerante a herbicidas para produção de biofertilizante: uma revisão baseada na metodologia Methodi ordinatio

The increasing growth in scientific publications makes researching more extensive in relation to the selection of papers to support the writing, which is time-consuming and requires criteria to effectively list the most important references. Microalgae have been studied by researchers in several technological fields in the development of products, because they have easy physiological adaptability, high productivity in biomass and low cost of cultivation. Among the applications of microalgae, it is mentioned in this work the production of biofertilizers which soften the effects caused by chemical agents to plants and the environment, aiming at the production of organic food, one of the great current challenges. The objective of this work is to use a systematic review of the literature on the cultivation of herbicide resistant microalgae using Methodi Ordinatio, which assists the search, selection, collection and classification of scientific articles. Initially, research was done on the data bases Scopus, Science direct and Web of Science, using the keywords microalgae and herbicide. The results were imported to the Mendeley bibliographic reference manager. Subsequently, the In Ordinatio index was applied, an equation that works with the three most important factors in a scientific article: the impact factor, the year of publication and the number of citations of the research. This equation turns it is possible to classify the most important articles in the area of herbicide-tolerant microalgae and note the importance of this method, which made it possible to find current studies and demonstrate the relevance of this research theme. In order to show the dynamics of the methodology, a research with the theme is presented, comprising works from years 2008 to 2018. The results indicate that the methodology is effective in relation to the objectives proposed, and the most relevant work on the cultivation of herbicide resistant microalgae are used to construct the scenario in this theme.info:eu-repo/semantics/publishedVersio

Obtaining microalgae biomass tolerant to herbicides for production of bio-fertilizers: a review based on methodi ordinatio methodology

The objective of this work was to use a systematic review of the literature on the cultivation of microalgae resistant to herbicide using the Method Methodi Ordinatio, which assists the search, selection, collection and classification of scientific articles. Initially, research was done on the databases Scopus, Science Direct and Web of Science, using the keywords “microalgae” and “herbicide”. The results were imported to the Mendeley bibliographic reference manager. Subsequently, the index In Ordinatio index was applied, which works with the three most important factors in a scientific article: The impact factor, the year of publication and the number of citations of the research. This index turns it is possible to classify the most important articles in the area of herbicide-tolerant microalgae and note the importance of this method, which made it possible to find current studies and demonstrate the relevance of this research theme. In order to show the dynamics of the methodology, a research with the theme is presented, comprising works from years 2008 to 2018. The results indicate that the methodology is effective in relation to the objectives proposed, and the most relevant work on the cultivation of herbicide resistant microalgae are used to construct the scenario in this theme.info:eu-repo/semantics/publishedVersio

Myiasis of a perineal fistula

We report a case of myiasis of a rectocutaneous fistula in a subject suffering from bone metastases after surgical resection of a neoplastic bladder The fistula was infested by maggots identified as third-instar larvae of flies belonging to the family Sarcophagidae, genus Sarcophaga. The infestation was neither intestinal nor pseudointestinal; it was probably caused by a fly ovipositing on the fistula of the patient while having wound care at home, even though the possibility that the infestation might have occurred during hospitalization cannot be ruled out

Molecular identification of Borrelia valaisiana and HGE-like Ehrlichia in Ixodes ricinus ticks sampled in North-Eastern Italy: first report in Veneto region

PCR amplification was applied to screen the presence of both Borrelia burgdorferi s.l. and Ehrlichia species in pools of field-collected Ixodes ricinus ticks. The specimens so far analysed (n = 55), grouped in 11 pools, were sampled in Feltre area (Veneto region, NE Italy). Five pools proved positive for B. valaisiana (45%) and one of them (9%) was also positive for Ehrlichia, that was further characterised as a HGE-like Ehrlichia. This is the first report of the two bacteria in the Veneto region. The pool positive for both pathogens was used to adjust a multiplex PCR assay, which allowed the detection and identification of both parasites in a single experiment. The advantages offered by this assay, when standardised, will substantially broaden the perspectives of ecological and epidemiological investigations on animal/human Lyme disease and ehrlichiosis, greatly facilitating disease surveillance and control programs

Borrelia burgdorferi s.l. and Ehrlichia chaffeensis in the National Park of Abruzzo

The aims of the present study were (a) to determine the presence of Ixodes ricinus in three different areas of the National Park of Abruzzo; (b) to search for the presence of Borrelia burgdorferi in the collected sample of Ixodes; (c) to determine the seroprevalence of B. burgdorferi antibodies and E. chaffeensis antibodies in inhabitants of the park and in park workers. The presence of B. burgdorferi in Ixodes was checked by PCR. For the detection of antibodies to B. burgdorferi all sera were assayed by ELISA as screening test and by Western blot as confirmatory test. For the detection of antibodies to E. chaffeensis all sera were assayed by IFA. Antibodies to B. burgdorferi were present in 9.1% of the park workers, 4.5% were confirmed positive by the IgG Western blot test. None of the inhabitants of the park was positive. Antibodies against E. chaffeensis were found in 4.5% of the park workers and 8% of the inhabitants of the park. The results obtained in the collecting of the ticks seem to show that the presence of I. ricinus in the park territory is rather discontinuous and small in number, therefore it is not epidemiologically significant for the transmission of B. burgdorferi sensu lato. Serological study for Ehrlichia revealed a high frequency of E. chaffeensis antibodies in the park inhabitants and a lower prevalence in the park workers

Occurrence of the G3 Indian buffalo strain of echinococcus granulosus in cattle

During a survey carried out to define the occurrence of Echinococcus granulosus in cattle bred in the province of Rieti (Central Italy), molecular diagnostics (PCR amplification and sequencing of a partial region of the mitochondrial C01 gene) showed that 6/10 positive bovines harboured hydatid cysts (No.=16) genetically identical (95.8-100%) to the Indian buffalo genotype G3. As far the location of the 16 cysts, 11 of them were found in the lungs of three animals, whereas 5 cysts were in the liver of three parasitized hosts. The occurrence of genotype G3 in 60% of parasitized bovines living in an area never studied before provides more definite evidence about the existence of the strain in this region, and proves that cattle have to be considered a non-accidental host