51 research outputs found
Identification of candidate genes involved in wax deposition in Poa pratensis by RNA-seq
Long-chain aliphatic wax esters isolated from the sponge Chalinula saudensis (Demospongia) along the Jeddah coast of the Red Sea
Acetyl-acyl carrier protein is not a major intermediate in fatty acid biosynthesis in spinach
Site-directed mutagenesis of the spinach acyl carrier protein-I prosthetic group attachment site
Co-purification, co-imniunoprecipitation, and coordinate expression of acetyl-coenzyme A carboxylase activity, biotin carboxylase, and biotin carboxyl carrier protein of higher plants
The maize glossy13 gene, cloned via BSR-Seq and Seq-Walking encodes a putative ABC transporter required for the normal accumulation of epicuticular waxes
Citation: Li, L., . . . & Schnable, Patrick. (2013). The Maize glossy13 Gene, Cloned via BSR-Seq and SeqWalking Encodes a Putative ABC Transporter Required for the Normal Accumulation of Epicuticular Waxes. PLoS ONE, 8(12), e82333.
https://doi.org/10.1371/journal.pone.0082333Aerial plant surfaces are covered by epicuticular waxes that among other purposes serve to control water loss. Maize glossy mutants originally identified by their “glossy” phenotypes exhibit alterations in the accumulation of epicuticular waxes. By combining data from a BSR-Seq experiment and the newly developed Seq-Walking technology, GRMZM2G118243 was identified as a strong candidate for being the glossy13 gene. The finding that multiple EMS-induced alleles contain premature stop codons in GRMZM2G118243, and the one knockout allele of gl13, validates the hypothesis that gene GRMZM2G118243 is gl13. Consistent with this, GRMZM2G118243 is an ortholog of AtABCG32 (Arabidopsis thaliana), HvABCG31 (barley) and OsABCG31 (rice), which encode ABCG subfamily transporters involved in the trans-membrane transport of various secondary metabolites. We therefore hypothesize that gl13 is involved in the transport of epicuticular waxes onto the surfaces of seedling leaves
Overproduction of a Functional Fatty Acid Biosynthetic Enzyme Blocks Fatty Acid Synthesis in Escherichia coli
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