Effect of Hyperbaric Storage at Room Temperature on the Activity of Polyphenoloxidase in Model Systems and Fresh Apple Juice

The effect of hyperbaric storage (HS) on polyphenoloxidase activity (PPO) was studied in model solutions and apple juice. Model solutions containing increasing amounts of mushroom (Agaricus bisporus) PPO (up to 26 U) were stored at room temperature at pressure up to 200 MPa. During HS, samples were assessed for residual PPO activity. The enzyme was completely inactivated according to a first-order kinetic model that was used to calculate PPO decimal reduction time (Dp) and pressure sensitivity (zp = 140.8 MPa) in diluted model solutions (2 U PPO). The increase in enzyme concentration (6–26 U) nullified the effect of HS, probably due to protein structure stabilization by self-crowding. The application of HS at 100 and 200 MPa to apple juice promoted a decrease in total bacteria, lactic acid bacteria, yeasts, and molds. These changes occurred in concomitance with the inactivation of PPO (zp = 227.3 MPa). At 200 MPa, PPO inactivation followed a biphasic first-order kinetic, suggesting the presence of PPO isozymes with different pressure sensitivity. The inactivation of PPO was observed to occur more rapidly with increasing storage pressure and led to the maintenance of the original bright juice color. This study proves the capability of HS to control enzyme-related quality decay in fruit juices and, potentially, in many other food matrices suffering enzymatic alteration

ZB−L′Z^{\prime}_{B-L} phenomenology at LHC

We study the $Z^\prime$ phenomenology for two extensions of the Electroweak Standard Model (SM) which have an extra $U(1)_{B-L}$ gauge factor. We show the capabilities of the LHC in distinguishing the signals coming from these two extensions and both of them from the Standard Model background. In order to compare the behavior of these $B-L$ models we consider the reaction $p + p\longrightarrow \mu^+ + \mu^- + X$ and compute some observables as the total cross sections, number of events, forward-backward asymmetry, final particle distributions like rapidity, transverse momentum, and dimuon invariant mass, for two LHC regimes: $\sqrt{s}\,({\cal L})=7$ TeV ($1\, \textrm{fb}^{-1}$) and $14$ TeV ($100\, \textrm{fb}^{-1}$) for $M_{Z^{\prime}}$ = 1000 GeV and 1500 GeV. We show that by using appropriate kinematic cuts some of the observables considered here are able to extract different properties of the $Z^\prime$ boson, and hence providing information about to which $B-L$ model it belongs to.Comment: 21 pages, 17 figures, 4 table

Characterization of high- And low-risk hepatocellular adenomas by magnetic resonance imaging in an animal model of glycogen storage disease type 1A

Hepatocellular adenomas (HCAs) are benign tumors, of which the most serious complications are hemorrhage and malignant transformation to hepatocellular carcinoma (HCC). Among the various subtypes of HCA, the \u3b2-catenin-activated subtype (bHCA) is associated with greatest risk of malignant transformation. Magnetic resonance imaging (MRI) is an important tool to differentiate benign and malignant hepatic lesions, and preclinical experimental approaches may help to develop a method to identify MRI features associated with bHCA. HCAs are associated with various pathologies, including glycogen storage disease 1a (GSD1a). Here, we utilized a mouse model for GSD1a that develops HCA and HCC, and analyzed the mice in order to distinguish low-risk from high-risk tumors. Animals were scanned by MRI using a hepato-specific contrast agent. The mice were sacrificed after MRI and their lesions were classified using immunohistochemistry. We observed that 45% of the animals developed focal lesions, and MRI identified four different patterns after contrast administration: isointense, hyperintense and hypointense lesions, and lesions with peripheral contrast enhancement. After contrast administration, only bHCA and HCC were hypointense in T1-weighted imaging and mildly hyperintense in T2-weighted imaging. Thus, high-risk adenomas display MRI features clearly distinguishable from those exhibited by low-risk adenomas, indicating that MRI is a reliable method for early diagnosis and classification of HCA, necessary for correct patient management

Análise de componentes principais de atributos químicos e físicos do solo limitantes à produtividade de grãos.

O objetivo deste trabalho foi avaliar, por meio da análise dos componentes principais, a redução na dimensionalidade de atributos químicos e físicos do solo para a compreensão da variabilidade espacial e temporal da produtividade de culturas de grãos. A área experimental, de 54 ha, é manejada em agricultura de precisão há oito anos. Com base em seis mapas de colheita (soja - safra 2000/2001; milho - 2001/2002; soja - 2002/2003; trigo - 2003; soja - 2003/2004; e milho - 2004/2005), a área foi dividida em três zonas de produtividade de grãos (alta, média e baixa). Foram definidos 15 pontos georreferenciados representativos, para determinação de atributos químicos e físicos do solo, o que totalizou 63 variáveis analisadas. Entre os atributos químicos, o elevado teor de K no solo é o que melhor explica a variabilidade espacial da produtividade das culturas de grãos, provavelmente em razão do desbalanço das relações Ca:K e Mg:K. A zona de baixa produtividade apresentou baixa qualidade física do solo. Neste caso, a infiltração de água no solo, isoladamente, é a variável que melhor explica o desempenho das culturas de grãos. A análise dos componentes principais dos atributos químicos e físicos do solo é estratégia eficiente para explicar a variabilidade espacial e temporal da produtividade de culturas de grãos

Agricultura de precisão no manejo de pragas na cultura da soja no sul do Brasil.

Entre os fatores que podem interferir negativamente no rendimento de grãos da soja, destacam-se os insetos-praga. O estudo da distribuição espacial de insetos-praga é fundamental para a utilização de estratégias de controle, aperfeiçoamento de técnicas de amostragens, quantificação de danos econômicos e incorporação de um programa de agricultura de precisão (AP) voltado para manejo integrado de pragas. Avaliar a distribuição espacial de pragas na cultura da soja por meio de ferramentas de AP e a possibilidade de controle sítio específico foi o objetivo desse estudo. O estudo foi realizado na safra agrícola 2007/2008 em uma área de 99,75 ha, localizada no município de Boa Vista das Missões, RS, Brasil. A área foi amostrada em malha de 100 m x 100 m (1 ha) totalizando 98 pontos amostrais. Para fins de comparação, 40,27 ha foram manejados segundo princípios da AP e 59,48 ha segundo a metodologia convencional. Durante a safra investigada realizaram-se 13 avaliações de pragas semanalmente. Este estudo indicou que a utilização das ferramentas de AP em associação as do Manejo Integrado de Pragas - MIP mostram-se promissoras na redução do custo de produção e no incremento da sustentabilidade da produção de soja

Effects of miRNA-15 and miRNA-16 expression replacement in chronic lymphocytic leukemia : implication for therapy

This work was supported by: Associazione Italiana Ricerca sul Cancro (AIRC) Grant 5 x mille n.9980, (to M.F., F.M. A. N., P.T. and M.N.) ; AIRC I.G. n. 14326 (to M.F.), n.10136 and 16722 (A.N.), n.15426 (to F.F.). AIRC and Fondazione CaRiCal co-financed Multi Unit Regional Grant 2014 n.16695 (to F.M.). Italian Ministry of Health 5x1000 funds (to S.Z. and F.F). A.G R. was supported by Associazione Italiana contro le Leucemie-Linfomi-Mielomi (AIL) Cosenza - Fondazione Amelia Scorza (FAS). S.M. C.M., M.C., L.E., S.B. were supported by AIRC.Peer reviewedPostprin

Drug resistance in B and non-B subtypes amongst subjects recently diagnosed as primary/recent or chronic HIV-infected over the period 2013–2016: Impact on susceptibility to first-line strategies including integrase strand-transfer inhibitors

Objectives To characterize the prevalence of transmitted drug resistance mutations (TDRMs) by plasma analysis of 750 patients at the time of HIV diagnosis from January 1, 2013 to November 16, 2016 in the Veneto region (Italy), where all drugs included in the recommended first line therapies were prescribed, included integrase strand transfer inhibitors (InNSTI). Methods TDRMs were defined according to the Stanford HIV database algorithm. Results Subtype B was the most prevalent HIV clade (67.3%). A total of 92 patients (12.3%) were expected to be resistant to one drug at least, most with a single class mutation (60/68–88.2% in subtype B infected subjectsand 23/24–95.8% in non-B subjects) and affecting mainly NNRTIs. No significant differences were observed between the prevalence rates of TDRMs involving one or more drugs, except for the presence of E138A quite only in patients with B subtype and other NNRTI in subjects with non-B infection. The diagnosis of primary/recent infection was made in 73 patients (9.7%): they had almost only TDRMs involving a single class. Resistance to InSTI was studied in 484 subjects (53 with primary-recent infection), one patient had 143C in 2016, a total of thirteen 157Q mutations were detected (only one in primary/recent infection). Conclusions Only one major InSTI-TDRM was identified but monitoring of TDRMs should continue in the light of continuing presence of NNRTI-related mutation amongst newly diagnosed subjects, sometime impacting also to modern NNRTI drugs recommended in first-line therapy

The mirror module design for the cryogenic x-ray imaging spectrometer on-board ORIGIN

ORIGIN is a medium size high-energy mission concept submitted to ESA in response to the Cosmic Vision call issued on July 2010. The mission will investigate the evolution of the Universe by performing soft X-ray high resolution spectroscopic measurements of metals formed in different astrophysical environments, from the first population III stars at z > 7 to the present large scale structures. The main instrument on-board ORIGIN will be a large format array of TES X-ray micro-calorimeters covering a FOV of 30' at the focal plane of a grazing incidence optical module with a focal length of 2.5 m and an angular resolution of 30'' HEW at 1 keV. We present the optical module design which is based on hybrid technologies, namely Silicon Pore Optics for the outer section and Ni electro-forming for the inner section, and we present the expected performances based on test measurements and ray-tracing simulations

MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita.

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