Antibiotic Susceptibility of Lactic Acid Bacteria Isolated From Cow, Goat, Donkey, Buffalo, Sheep, Camel and Human Milk

Breast milk is a very important factor in regulating gastrointestinal function, improving the immune system and preventing acute illnesses (e.g. acute otitis media), especially during breastfeeding. Breast milk allows beneficial bacteria such as bifidobacteria and lactobacillus species to colonise the newborn intestin*e instead of potential enteropathogenic bacteria such as streptococci and escherichia coli. The aim of the study was to compare the antibiotic resistance of Lactic acid bacteria members isolated from different milk samples. Six milk samples were collected from each of seven different milk sources (donkey, goat, cow, buffalo, sheep, camel and human). The MRS and M-17 medium were used with the double layer sandwich method for isolation of LAB members selected from typic colonies, gram-positive, catalase-negative used in the study. The isolated 42 LAB species were determined antibiotic susceptibility with 9 standart antibiotic discs such as tetracycline, penicillin, kanamycin, streptomycin, rifampin, gentamicin, chloramphenicol, teicoplanin, ciprofloxacin by agar disc diffusion assay test. The 18 LAB isolate were resistant to penicillin, 16 isolate to kanamycin, 14 isolate to gentamicin. LAB isolates such as H1, H4, H5 from Human milk and LAB isolate such as D1 from donkey milk was considered as MDR isolates because they were resistant to at least four of the tested drugs. Among the milks, sheep, camel, buffalo and goat milks were found to be more sensitive to antibiotics on average in the group

Comparison of Community-Acquired Rotavirus Infection with Nosocomial Rotavirus Infection; Evaluation of Epidemiology, Severity and Economic Burdens

Aim: The aim of this study was to evaluate the demographic data of patients with community-acquired rotavirus (CAR) gastroenteritis, the incidence of patients with nosocomial rotavirus gastroenteritis (NRG), the length of hospitalization and direct costs of these two groups, and the effect of NRG in pediatric patients. Materials and Methods: The records of patients aged 0-144 months who were admitted to Halfeti State Hospital between July 2017 and July 2019 and who hospitalized for rotavirus gastroenteritis and patients who developed nosocomial rotavirus infection (NRI) while hospitalized for any reason were reviewed retrospectively. Demographic characteristics, laboratory data, direct cost analysis of patients with CAR infection and patients with NRI were examined and comparisons were made between these two groups. Results: A total of 195 patients were included in the study, of which 29 were patients with NRI. Patients in both groups were most frequently between 25-60 months. The length of hospitalization was on average 6 days more in patients with NRG. While the average hospitalization cost of patients with positive NRI was 175.2 USD, the average hospitalization cost of patients without NRI was 120.6 USD and this difference was statistically significant (p = 0.001). Conclusion: Rotavirus infectio; especially in children under the age of five which is common mostly in winter, can be highly nosocomial and creates a serious burden on the health system. Applications such as hand washing, the use of personal protective equipment of healthcare workers, patient isolation can reduce NRI

Comparison of Pro-BNP Levels and Cardiac Functions Before and After Iron Therapy in Children with Congenital Cyanotic Cardiac Disease and Iron Deficiency Anemia.

TEZ10371Tez (Uzmanlık) -- Çukurova Üniversitesi, Adana, 2016.Kaynakça (s. 63-72) var.x, 78 s. : tablo ; 29 cm.Amaç: Demir eksikliği anemisi olan doğumsal siyanotik kalp hastalıklı çocuklarda demir tedavisi sonrası NT-proBNP düzeyleri ve kardiyak fonksiyonların karşılaştırılmasıdır. Gereç ve Yöntem: Eylül 2015 - Mart 2016 tarihleri arasında Çukurova Üniversitesi Tıp Fakültesi Çocuk Kardiyoloji Bilim Dalı polikliniğine başvuran, siyanotik doğuştan kalp hastalığı tanılı, demir eksikliği anemisi saptanan altı ay ile 17 yaşları arasındaki 40 çocuk hasta çalışmaya alınmıştır. Çeşitli sebeplerden dolayı 26 hasta ile sonuçlar değerlendirilmiştir. Hastaların ilk geliş ve üçüncü ay kontrollerinde; demografik verileri, tam kan sayımı, periferik yayma, retikülosit, serum demir, total demir bağlama kapasitesi, ferritin düzeyleri, transferrin satürasyonlarıu, NT-proBNP düzeyleri ve Eko bulguları değerlendirilmiştir. Bulgular: Çalışma 26 hasta ile tamamlanmış olup yaş ortalaması 57,7± 54,6 aydı. Hastaların demir tedavisi öncesi ve sonrası laboratuvar değerlerinde; hemoglobin, hematokrit, MCV, MCHC, demir, ferritin, transferin saturasyonu, oksijen saturasyonu değerleri tedavi sonrası istatistiksel olarak anlamlı derece artmıştır. RDW ve NTproBNP düzeyleri yüksekliği kontrol sonuçlarında anlamlı olarak düşmüştür. Ekokardiyografi bulgularından; sol ventrikül sistolik fonksiyonlarından sol ventrikül EF ölçümlerinin ve sağ ventrikül diastolik çap ölçümlerinin değerlendirilmesinde sınırda anlamlı farklılık saptanmıştır. Sağ ventrikül diastolik volüm ölçümlerinin karşılaştırılmasında istatistiksel olarak anlamlı fark bulunmuştur. Sol ventrikül doku dopplerinde; MPİ ölçümlerinde 0. gün ile 90. gün arasında anlamlı farklılık saptanmıştır. Diğer EKO bulgularında anlamlı farklılık saptanmamıştır. Sonuç: Demir tedavisi sonrası siyanotik doğuştan kalp hastalıklı çocuklarda kardiyak fonksiyonlarda iyileşme saptanmış olup NT-proBNP takipte, tedavinin değerlendirilmesinde kullanılabilir.Aim: It is the comparison of NT-proBNP levels and cardiac functions after iron therapy in children with congenital cyanotic cardiac disease and iron deficiency anemia. Material and Method: Forty children between six months and 17 years old who were diagnosed with cyanotic congenital cardiac disease and iron deficiency anemia after referring to Çukurova University Faculty of Medicine Child Cardiology Department polyclinic between September 2015 and March 2016 were included in the study. Results for 26 patients were evaluated due to different reasons. Demographical data, total blood count, peripheral smear, reticulocyte, serum iron, total iron binding capacity, ferritin levels, transferrin saturations, NT-proBNP levels and Echo findings were evaluated in the first visit and third month controls of the patients. Findings: The study was completed with 26 patients and mean age was 57.7± 54.6 months. Laboratory values of the patients before and after iron therapy; hemoglobin, hematocrit, MCV, MCHC, iron, ferritin, transferrin saturation, oxygen saturation values increased statistically significantly after the theraphy. High RDW and NT-proNP levels decreased significantly in control results. Among ECHO findings, a borderline statistically significant difference was detected in the evaluation of left ventricular EF measurements among left ventricular systolic functions and right ventricular diastolic diameter measurements. A statistically significant difference was found in the comparison of right ventricular diastolic volume measurements. There was a significant difference in MPI measurements in left ventricule tissue doppler between 0 and 90th days. No significant difference was detected in other ECHO findings. Result: After iron therapy, recovery was detected in the cardiac functions of the children with cyanotic cardiac disease and NT-proBNP can be used in follow-up and evaluation of the treatment.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: TTU2015-4408

Comparison Of Pro-BNP Levels And Myocardial Performance Index Before And After Iron Treatment In Children With Congenital Cyanotic Heart Disease With Iron Deficiency Anemia

Introduction: Our aim was to compare NT-proBNP levels and cardiac functions after iron therapy in children with congenital cyanotic heart disease who had iron deficiency anemia.Materials and Methods: We included 40 children with pre-established cyanotic congenital heart disease and accompanying iron deficiency anemia, between the age of 6 months and 17 years, who were admitted to the outpatient clinic of Çukurova University Faculty of Medicine, Department of Pediatric Cardiology between September 2015 and March 2016. We recorded demographic data and performed following investigations: complete blood count, peripheral blood smear, reticulocyte count, measurement of serum iron levels, total iron-binding capacity, ferritin levels, transferrin saturation and NT-proBNP levels, and echocardiographic examination.Results: There was a statistically significant increase in following laboratory parameters after iron therapy: hemoglobin, hematocrit, MCV, MCHC, serum iron and ferritin, transferrin saturation and oxygen saturation. During the follow-up period, RDW and NT-proBNP levels were significantly decreased. In left ventricular tissue doppler; there was a significant difference in MPI measurements before and after treatment. There was no significant difference in other echocardiography findings. Conclusion: Iron therapy has improved cardiac functions in children with cyanotic congenital heart disease, and NT-proBNP levels can be used to evaluate the efficacy of treatment in the follow-up period