Il settore dell'olivicoltura da tavola in Italia: situazione e prospettive

The Italian table olive industry is almost completely located in the southern regions: Sicily and Apulia together account for about 2/3 of the entire national production. ‘Nocellara del Belice’,‘Nocellara etnea’, ‘Bella di Cerignola’ and ‘Ascolana tenera’ are the leading cultivars together with a large number of other local traditional varieties, often utilised for a double purpose (table olives and oil production). Although Italy is nowadays largely dependent (for about 60%) onto import from other Countries (mainly Spain, Greece and Morocco) the domestic production has been declining in the last thirty years and currently accounts for about 3-4% of the total world production against a value of about 10% of the period 1980/85. Nevertheless, some interesting examples of specialized production concentrations are still represented by the producing areas of the ‘Nocellara del Belice’, ‘Bella di Cerignola’ (syn. ‘Bella della Daunia’) and ‘Ascolana tenera’ which recently received the DOP (protected designation of origin) recognition. In this paper, besides the overall review of the Italian varietal platform and a brief description of the main cultivars, the advantages and the opportunities offered by the application of specialized cultural techniques are discussed, with special emphasis on the positive role of a more appropriate and wide diffusion of irrigation (including RDI) and chemical fruit thinning. Potential of other cultural techniques such as fertilization, pruning and pest and disease control are also reviewed with reference to the possible role in fruit quality enhancement together with the needs for further development of the entire table olive industry and the R&D involved

Seasonal effects on mortality rates and resprouting of stems treated with glyphosate in the invasive tree of heaven (Ailanthus altissima (Mill.) Swingle)

Tree of heaven (Ailanthus altissima) is regarded as invasive within urban and natural areas worldwide. Efficient methods to control it are significantly needed if we are to limit its well-known environmental and economic impacts. Up to now the use of herbicides has proven necessary since following mechanical damage, Ailanthus vigorously resprouts. However, the seasonal response of Ailanthus stems, treated with herbicides, has never been assessed. We compared the control efficacy recorded in autumn, winter and summer in an abandoned suburban citrus grove in Sicily, under Mediterranean-climate conditions. Glyphosate was injected within drill holes made in knee-high cut trees. Tree mortality and the resprouting ability of treated stems were significantly affected by the application timing and by diameter class of stems. Mortality was significantly lower in winter treatments within the smaller diameter class. Within the larger diameter class, only autumn treatments showed a notably higher mortality rate and a significantly lower growth of sprouts. Hence, autumn interventions are strongly recommended to effectively treat Ailanthus. Mortality was notably higher in the smaller diameter class. Our results confirm the importance of early intervention to control Ailanthus, also showing that the choice of the season is crucial to obtaining satisfactory control of its resprouting ability

The Italian Almond Industry: New Perspectives and Ancient Tradition

The almond industry in Italy presents a high degree of structural variability that is determined by the jeopardized coexistence of old, traditional orchards and new plantations that differ each other not only in terms of age but also mainly in terms of orchard system. Apulia and Sicily still remain the most important production areas where the 90% of the almond orchards are concentrated. In this southern part of Italy, almond and its derivatives still remain really important. In the last two decades, the Italian almond industry did not show a regular trend, neither with respect to production nor to acreage. An innovative almond industry revealed its consistency year after year since 2000: self-fertile cultivars, clonal rootstocks and new planting design ideal for mechanization were the most important technical aspects which revealed a new way to set up almond orchards. As a matter of fact, the Italian almond industry is still characterized by two strongly different structures: a traditional one, which is based upon old cultivars and orchard systems, with unsatisfactory yields indeed, and an innovative one which is really interesting and appears to be able to partially renew the sector. Technical aspects on this new way to obtain innovative almond orchards and their perspectives are here reported mostly in terms of technical choices

First results on the phenotypic analysis of wild and cultivated species of Pyrus in Sicily

Phenotypic analysis of Pyrus in Sicily using a set of 19 characters on 71 accessions belonging to 7 species is here reported. Results show a high level of morphological variation in this genus. Cluster analysis of the morphological characters allows to identify two groups: the first closely related to wild pears and the latter related to P. communis s. l. and P. sicanorum. It is likely to think that many Ethno-Varieties, usually attributed to P. communis, on the contrary are attributable to wild Sicilian pears and belong to indigenous germplasm

Thiazolidin-4-one formation. Mechanistic and synthetic aspects of the reaction of imines and mercaptoacetic acid under microwave and conventional heating

Microwave irradiation of a mixture of benzylidene-anilines and mercaptoacetic acid in benzene gives 1,3-thiazolidin-4- ones in very high yield (65–90%), whereas the same reaction performed through using the conventional method, at refluxtemperature, requires a much longer time and gives a much lower yield (25–69%). This difference seems to be due to someintermediates and by-products formed during the conventional reaction. On the basis of 1H NMR studies, two differentmechanisms, acting in benzene and in DMF, respectively, have been hypothesized for the thiazolidin-4-one system formation

Antitumor Agents. 2. Synthesis, Structure-Activity Relationships, and Biological Evaluation of Substituted 5H-Pyridophenoxazin-5-ones with Potent Antiproliferative Activity

New antiproliferative compounds, 5H-pyrido[3,2-a]phenoxazin-5-ones (1-10), 5H-benzophenoxazin- 5-one (11), 5H-pyrido[2,3-a]phenoxazin-5-one (12), 5H-pyrido[3,4-a]phenoxazin-5-one (13), and 5H-pyrido[4,3-a]phenoxazin-5-one (14), were synthesized and evaluated against representative human neoplastic cell lines. The excellent cytotoxic activity of these polycyclic phenoxazinones, structurally related to the actinomycin chromophore, is discussed in terms of structural changes made to rings A and D (Chart 1). Electron-withdrawing or electron-donating substituents were introduced at different positions of ring A to probe the electronic and positional effects of the substitution. A nitro group in R2 or in R1 increases the cytotoxic activity, whereas electron-donating methyl groups in any position lead to 10- to 100-fold decreasing of the activity. The low antiproliferative activity of benzophenoxazinone 11 and pyridophenoxazinones 13 and 14 confirms the crucial role of pyridine nitrogen in the W position of ring D in DNA binding. The unexpected high activity exhibited by 12, which has the nitrogen in the X position, could be ascribed to a different mechanism of action, which needs further investigation

Hormone-dependent nuclear export of estradiol receptor and DNA synthesis in breast cancer cells

In breast cancer cells, cytoplasmic localization of the estradiol receptor α (ERα) regulates estradiol-dependent S phase entry. We identified a nuclear export sequence (NES) in ERα and show that its export is dependent on both estradiol-mediated phosphatidylinositol-3-kinase (PI3K)/AKT activation and chromosome region maintenance 1 (CRM1). A Tat peptide containing the ERα NES disrupts ERα–CRM1 interaction and prevents nuclear export of ERα- and estradiol-induced DNA synthesis. NES-ERα mutants do not exit the nucleus and inhibit estradiol-induced S phase entry; ERα-dependent transcription is normal. ERα is associated with Forkhead proteins in the nucleus, and estradiol stimulates nuclear exit of both proteins. ERα knockdown or ERα NES mutations prevent ERα and Forkhead nuclear export. A mutant of forkhead in rhabdomyosarcoma (FKHR), which cannot be phosphorylated by estradiol-activated AKT, does not associate with ERα and is trapped in the nucleus, blocking S phase entry. In conclusion, estradiol-induced AKT-dependent phosphorylation of FKHR drives its association with ERα, thereby triggering complex export from the nucleus necessary for initiation of DNA synthesis and S phase entry

Antitumor Agents. 1. Synthesis, Biological Evaluation, and Molecular Modeling of 5H-Pyrido[3,2-a]phenoxazin-5-one, a Compound with Potent Antiproliferative Activity

The iminoquinone is an important moiety of a large number of antineoplastic drugs and plays a significant role in the nucleus of actinomycins, powerful, highly toxic, natural antibiotics that target DNA as intercalating agents. A series of polycyclic iminoquinonic compounds, 2-amino-3H-phenoxazin-3-one (1), 2-amino-1,9-diacetyl-3H-phenoxazin-3-one (2), 2-acetylamino- 3H-phenoxazin-3-one (3), 3H-phenoxazin-3-one (4), 5H-pyrido[3,2-a]phenoxazin-5-one (5), and 5H-pyrido[3,2-a]phenothiazin-5-one (6), strictly related to the actinomycin chromophore, were synthesized for developing new anticancer intercalating drugs. The antiproliferative activity of these compounds, evaluated against representative human liquid and solid neoplastic cell lines, showed that 5 and its isoster 6 were the most active compounds inhibiting cell proliferation in a submicromolar range. Compound 5 was also evaluated against KB subclones (KBMDR, KB7D, and KBV20C), which overexpress the MDR1/P-glycoprotein drug efflux pump responsible for drug resistance. All the above KB subclones did not show altered sensitivity to the antiproliferative activity of 5. UV-vis and 1H NMR spectroscopy experiments support the phenoxazinone 5/DNA binding. Molecular mechanics methods were used to build a three-dimensional model of the 5/[d(GAAGCTTC)]2 complex. Electrostatic interactions between the hydrogen of the positively charged pyridine nitrogen of 5 and the negatively charged oxygen atoms (O4¢ and O5¢) of the cytosine C5 residue together with stacking forces contribute to the high antiproliferative activity. The metal(II)-assisted synthesis procedure of 5 is described, and the formation mechanism is proposed