The Tully-Fisher relation of distant field galaxies

We examine the evolution of the Tully-Fisher relation (TFR) using a sample of 89 field spirals, with 0.1 < z < 1, for which we have measured confident rotation velocities (Vrot). By plotting the residuals from the local TFR versus redshift, or alternatively fitting the TFR to our data in several redshift bins, we find evidence that luminous spiral galaxies are increasingly offset from the local TFR with redshift, reaching a brightening of -1.0+-0.5 mag, for a given Vrot, by approximately z = 1. Since selection effects would generally increase the fraction of intrinsically-bright galaxies at higher redshifts, we argue that the observed evolution is probably an upper limit. Previous studies have used an observed correlation between the TFR residuals and Vrot to argue that low mass galaxies have evolved significantly more than those with higher mass. However, we demonstrate that such a correlation may exist purely due to an intrinsic coupling between the Vrot scatter and TFR residuals, acting in combination with the TFR scatter and restrictions on the magnitude range of the data, and therefore it does not necessarily indicate a physical difference in the evolution of galaxies with different Vrot. Finally, if we interpret the luminosity evolution derived from the TFR as due to the evolution of the star formation rate (SFR) in these luminous spiral galaxies, we find that SFR(z) is proportional to (1+z)^(1.7+-1.1), slower than commonly derived for the overall field galaxy population. This suggests that the rapid evolution in the SFR density of the universe observed since approximately z = 1 is not driven by the evolution of the SFR in individual bright spiral galaxies. (Abridged.)Comment: 14 pages, 10 figures, accepted by MNRA

Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: a putative cytoplasmic ferri-siderophore reductase

YqjH is a cytoplasmic FAD-containing protein from Escherichia coli; based on homology to ViuB of Vibrio cholerae, it potentially acts as a ferri-siderophore reductase. This work describes its overexpression, purification, crystallization and structure solution at 3.0 A resolution. YqjH shares high sequence similarity with a number of known siderophore-interacting proteins and its structure was solved by molecular replacement using the siderophore-interacting protein from Shewanella putrefaciens as the search model. The YqjH structure resembles those of other members of the NAD(P)H:flavin oxidoreductase superfamily

MegaMorph: classifying galaxy morphology using multi-wavelength S\'ersic profile fits

Aims. This work investigates the potential of using the wavelength-dependence of galaxy structural parameters (S\'ersic index, n, and effective radius, Re) to separate galaxies into distinct types. Methods. A sample of nearby galaxies with reliable visual morphologies is considered, for which we measure structural parameters by fitting multi-wavelength single-S\'ersic models. Additionally, we use a set of artificially redshifted galaxies to test how these classifiers behave when the signal-to-noise decreases. Results. We show that the wavelength-dependence of n may be employed to separate visually-classified early- and late-type galaxies, in a manner similar to the use of colour and n. Furthermore, we find that the wavelength variation of n can recover galaxies that are misclassified by these other morphological proxies. Roughly half of the spiral galaxies that contaminate an early-type sample selected using (u-r) versus n can be correctly identified as late-types by N, the ratio of n measured in two different bands. Using a set of artificially-redshifted images, we show that this technique remains effective up to z ~ 0.1. N can therefore be used to achieve purer samples of early-types and more complete samples of late-types than using a colour-n cut alone. We also study the suitability of R, the ratio of Re in two different bands, as a morphological classifier, but find that the average sizes of both early- and late-type galaxies do not change substantially over optical wavelengths.Comment: 6 pages, 2 figures, 2 tables, Accepted for publication in A&

Star formation rates and chemical abundances of emission line galaxies in intermediate-redshift clusters

We examine the evolutionary status of luminous, star-forming galaxies in intermediate-redshift clusters by considering their star formation rates and the chemical and ionsiation properties of their interstellar emitting gas. Our sample consists of 17 massive, star-forming, mostly disk galaxies with M_{B}<-20, in clusters with redshifts in the range 0.31< z <0.59, with a median of =0.42. We compare these galaxies with the identically selected and analysed intermediate-redshift field sample of Mouhcine et al. (2006), and with local galaxies from the Nearby Field Galaxy Survey of Jansen et al. (2000). From our optical spectra we measure the equivalent widths of OII, Hbeta and OIII emission lines to determine diagnostic line ratios, oxygen abundances, and extinction-corrected star formation rates. The star-forming galaxies in intermediate-redshift clusters display emission line equivalent widths which are, on average, significantly smaller than measured for field galaxies at comparable redshifts. However, a contrasting fraction of our cluster galaxies have equivalent widths similar to the highest observed in the field. This tentatively suggests a bimodality in the star-formation rates per unit luminosity for galaxies in distant clusters. We find no evidence for further bimodalities, or differences between our cluster and field samples, when examining additional diagnostics and the oxygen abundances of our galaxies. This maybe because no such differences exist, perhaps because the cluster galaxies which still display signs of star-formation have recently arrived from the field. In order to examine this topic with more certainty, and to further investigate the way in which any disparity varies as a function of cluster properties, larger spectroscopic samples are needed.Comment: 10 pages, 6 figures, MNRAS in pres

The Tully-Fisher relation of intermediate redshift field and cluster galaxies from Subaru spectroscopy

We have carried out spectroscopic observations in 4 cluster fields using Subaru's FOCAS multi-slit spectrograph and obtained spectra for 103 bright disk field and cluster galaxies at $0.06 \le z \le 1.20$. Seventy-seven of these show emission lines, and 33 provide reasonably-secure determinations of the galaxies' rotation velocity. The rotation velocities, luminosities, colours and emission-line properties of these galaxies are used to study the possible effects of the cluster environment on the star-formation history of the galaxies. Comparing the Tully-Fisher relations of cluster and field galaxies at similar reshifts we find no measurable difference in rest-frame $B$-band luminosity at a given rotation velocity (the formal difference is $0.18\pm0.33$mag). The colours of the cluster emission line galaxies are only marginally redder in rest-frame $B-V$ (by $0.06\pm0.04$mag) than the field galaxies in our sample. Taken at face value, these results seem to indicate that bright star-forming cluster spirals are similar to their field counterparts in their star-formation properties. However, we find that the fraction of disk galaxies with absorption-line spectra (i.e., with no current star formation) is larger in clusters than in the field by a factor of $\sim3$--5. This suggests that the cluster environment has the overall effect of switching off star formation in (at least) some spiral galaxies. To interpret these observational results, we carry out simulations of the possible effects of the cluster environment on the star-formation history of disk galaxies and thus their photometric and spectroscopic properties. Finally, we evaluate the evolution of the rest-frame absolute $B$-band magnitude per unit redshift at fixed rotation velocity.Comment: 21 pages, 13 figures, accepted for publication in MNRA

Overcoming the false-minima problem in direct methods: Structure determination of the packaging enzyme P4 from bacteriophage φ13

The problems encountered during the phasing and structure determination of the packaging enzyme P4 from bacteriophage φ13 using the anomalous signal from selenium in a single-wavelength anomalous dispersion experiment (SAD) are described. The oligomeric state of P4 in the virus is a hexamer (with sixfold rotational symmetry) and it crystallizes in space group C2, with four hexamers in the crystallographic asymmetric unit. Current state-of-the-art ab initio phasing software yielded solutions consisting of 96 atoms arranged as sixfold symmetric clusters of Se atoms. However, although these solutions showed high correlation coefficients indicative that the substructure had been solved, the resulting phases produced uninterpretable electron-density maps. Only after further analysis were correct solutions found (also of 96 atoms), leading to the eventual identification of the positions of 120 Se atoms. Here, it is demonstrated how the difficulties in finding a correct phase solution arise from an intricate false-minima problem. © 2005 International Union of Crystallography - all rights reserved