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    Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ высоколикопиновых Π³ΠΈΠ±Ρ€ΠΈΠ΄ΠΎΠ² Ρ‚ΠΎΠΌΠ°Ρ‚Π° для Ρ‚Π΅ΠΏΠ»ΠΈΡ† с использованиСм Ρ‚Ρ€Π°Π΄ΠΈΡ†ΠΈΠΎΠ½Π½Ρ‹Ρ… ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² сСлСкции ΠΈ молСкулярных ΠΌΠ°Ρ€ΠΊΠ΅Ρ€ΠΎΠ²

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    Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (Β° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points.ΠΠΊΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΡΡ‚ΡŒ. ВысокоС содСрТаниС Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ… Ρ‚ΠΎΠΌΠ°Ρ‚Π° являСтся Π²Π°ΠΆΠ½Ρ‹ΠΌ сСлСкционным ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΎΠΌ ΠΏΡ€ΠΈ создании Π½ΠΎΠ²Ρ‹Ρ… сортов ΠΈ Π³ΠΈΠ±Ρ€ΠΈΠ΄ΠΎΠ². ИспользованиС молСкулярных ΠΌΠ°Ρ€ΠΊΠ΅Ρ€ΠΎΠ² ΠΊ аллСлям, Π΄Π΅Ρ‚Π΅Ρ€ΠΌΠΈΠ½ΠΈΡ€ΡƒΡŽΡ‰ΠΈΠΌ биосинтСз Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°, Π² сочСтании с Ρ‚Ρ€Π°Π΄ΠΈΡ†ΠΈΠΎΠ½Π½Ρ‹ΠΌΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°ΠΌΠΈ сСлСкции Π½Π° высокоСсодСрТаниСкаротиноидов,цСнныСтСхнологичСскиС ивкусовыСкачСстваплодов,Π°Ρ‚Π°ΠΊΠΆΠ΅ Π½Π° ΡƒΡΡ‚ΠΎΠΉΡ‡ΠΈΠ²ΠΎΡΡ‚ΡŒ ΠΊ комплСксу ΠΏΠ°Ρ‚ΠΎΠ³Π΅Π½ΠΎΠ², позволяСт ΠΎΡ†Π΅Π½ΠΈΡ‚ΡŒ ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠ°Π» накоплСния антиоксиданта ΠΈ Π±ΠΎΠ»Π΅Π΅ эффСктивно ΡΠΎΡΡ‚Π°Π²Π»ΡΡ‚ΡŒ ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ ΠΏΠΎ сСлСкции высоколикопиновых Ρ„ΠΎΡ€ΠΌ для условий Π·Π°Ρ‰ΠΈΡ‰Π΅Π½Π½ΠΎΠ³ΠΎ Π³Ρ€ΡƒΠ½Ρ‚Π°.ΠœΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠ°. ΠŸΡ€Π΅Π΄ΡΡ‚Π°Π²Π»Π΅Π½Ρ‹ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΠΎΡ†Π΅Π½ΠΊΠΈ сСлСкционного ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π° с Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹ΠΌΠΈ комбинациями Π°Π»Π»Π΅Π»Π΅ΠΉ, Π΄Π΅Ρ‚Π΅Ρ€ΠΌΠΈΠ½ΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ… Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ², ΠΈ создания Π³ΠΈΠ±Ρ€ΠΈΠ΄ΠΎΠ² Ρ‚ΠΎΠΌΠ°Ρ‚Π° с комплСксом Ρ†Π΅Π½Π½Ρ‹Ρ… ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΎΠ² – высокой продуктивности, устойчивости ΠΊ болСзням, тСхнологичСских характСристик ΠΈ биохимичСского состава ΠΏΠ»ΠΎΠ΄ΠΎΠ². ΠœΠ΅Ρ‚ΠΎΠ΄ интрогрСссии спонтанных ΠΈΠ»ΠΈ ΠΈΠ½Π΄ΡƒΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… ΠΌΡƒΡ‚Π°Ρ†ΠΈΠΉ Π±Ρ‹Π» использован для ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΡ уровня ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² (og ΠΈ hp) ΠΈ ΡƒΠ»ΡƒΡ‡ΡˆΠ΅Π½ΠΈΡ тСхнологичСских качСств (nor, alc, rin) ΠΏΠ»ΠΎΠ΄ΠΎΠ². ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»ΠΎΠΌ для исслСдований являлся ΠΊΠΎΠ»Π»Π΅ΠΊΡ†ΠΈΠΎΠ½Π½Ρ‹ΠΉ, ΠΌΡƒΡ‚Π°Π½Ρ‚Π½Ρ‹ΠΉ, сСлСкционный, Π³ΠΈΠ±Ρ€ΠΈΠ΄Π½Ρ‹ΠΉ ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π» Ρ‚ΠΎΠΌΠ°Ρ‚Π° ΠΈ внСсСнныС Π² ГосрССстр Π Π€ Π³ΠΈΠ±Ρ€ΠΈΠ΄Ρ‹ Ρ‚ΠΎΠΌΠ°Ρ‚Π° сСлСкции Π‘Π‘ Агрофирмы Β«Π˜Π»ΡŒΠΈΠ½ΠΈΡ‡Π½Π°Β» – Π’ΠΠ˜Π˜Πž Ρ„ΠΈΠ»ΠΈΠ°Π» ЀГБНУ ЀНЦО. Π”ΠΠš-Ρ‚ΠΈΠΏΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠ² качСства ΠΏΠ»ΠΎΠ΄ΠΎΠ² выполняли Π² Π˜Π½ΡΡ‚ΠΈΡ‚ΡƒΡ‚Π΅ Π³Π΅Π½Π΅Ρ‚ΠΈΠΊΠΈ ΠΈ Ρ†ΠΈΡ‚ΠΎΠ»ΠΎΠ³ΠΈΠΈ НАН БСларуси.Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹. Π‘ΠΎΠ·Π΄Π°Π½Ρ‹ отСчСствСнныС Π³ΠΈΠ±Ρ€ΠΈΠ΄Ρ‹ для ΠΏΡ€ΠΎΠΌΡ‹ΡˆΠ»Π΅Π½Π½Ρ‹Ρ… Ρ‚Π΅ΠΏΠ»ΠΈΡ† с высокими вкусовыми ΠΈ тСхнологичСскими качСствами с использованиСм поэтапных скрСщиваний, ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ²ΡˆΠΈΡ… ΠΎΠ±ΡŠΠ΅Π΄ΠΈΠ½ΠΈΡ‚ΡŒ Π³Π΅Π½Ρ‹ nor, rin, alc, приводящиС ΠΊ ΡƒΠ΄Π»ΠΈΠ½Π΅Π½ΠΈΡŽ сроков хранСния с Π³Π΅Π½Π°ΠΌΠΈ B, og, hp1 ΠΈ Π΄Ρ€., ΡΠΏΠΎΡΠΎΠ±ΡΡ‚Π²ΡƒΡŽΡ‰ΠΈΠΌΠΈ ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΡŽ содСрТания ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² ΠΏΠ»ΠΎΠ΄Π°Ρ…. УстановлСно, Ρ‡Ρ‚ΠΎ ΠΏΡ€ΠΈ Ρ†Π΅Π»Π΅Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½Π½ΠΎΠΌ ΠΎΡ‚Π±ΠΎΡ€Π΅ ΠΈ Π³ΠΈΠ±Ρ€ΠΈΠ΄ΠΈΠ·Π°Ρ†ΠΈΠΈ, нСсмотря Π½Π° Π½Π΅Π³Π°Ρ‚ΠΈΠ²Π½ΠΎΠ΅ влияниС Π³Π΅Π½ΠΎΠ² nor, rin, alc Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΠΏΠΎΠ΄Π½ΡΡ‚ΡŒ ΡƒΡ€ΠΎΠ²Π΅Π½ΡŒ ΠΊΠ°Ρ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² Π΄ΠΎ срСдних Π²Π΅Π»ΠΈΡ‡ΠΈΠ½. ΠŸΠΎΠ΄Ρ‚Π²Π΅Ρ€ΠΆΠ΄Π΅Π½Π° коррСляция уровня Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ… с ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ΠΌ Ρ‚Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹ ΠΈ уровня инсоляции. Показано, Ρ‡Ρ‚ΠΎ Ρ€ΠΎΠ·ΠΎΠ²ΠΎΠΏΠ»ΠΎΠ΄Π½Ρ‹Π΅ Ρ„ΠΎΡ€ΠΌΡ‹ содСрТат Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ большСС количСство Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°, Ρ‡Π΅ΠΌ красноплодныС. ΠžΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½Ρ‹ сочСтания Π°Π»Π»Π΅Π»Π΅ΠΉ структурных Π³Π΅Π½ΠΎΠ² биосинтСза ΠΊΠΎΡ€ΠΎΡ‚ΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΈ рСгуляторных Π³Π΅Π½ΠΎΠ², ΠΎΠ±Π΅ΡΠΏΠ΅Ρ‡ΠΈΠ²Π°ΡŽΡ‰ΠΈΡ… максимальноС Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Ρƒ Π³ΠΈΠ±Ρ€ΠΈΠ΄ΠΎΠ² с красными ΠΈ Ρ€ΠΎΠ·ΠΎΠ²Ρ‹ΠΌΠΈ ΠΏΠ»ΠΎΠ΄Π°ΠΌΠΈ, Ρ„ΠΎΡ€ΠΌΡƒΠ»Ρ‹ ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… Ρ€Π°Π·Π»ΠΈΡ‡Π°Π»ΠΈΡΡŒ ΠΏΠΎ составу Π°Π»Π»Π΅Π»Π΅ΠΉ Π³Π΅Π½ΠΎΠ² качСства ΠΏΠ»ΠΎΠ΄ΠΎΠ². Π’Ρ‹Π΄Π΅Π»Π΅Π½Ρ‹ Π³ΠΈΠ±Ρ€ΠΈΠ΄Ρ‹ с сочСтаниСм высоких ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΉ сахара (Β°Brix), сухого вСщСства ΠΈ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡŒΠ½Ρ‹Ρ… Π·Π½Π°Ρ‡Π΅Π½ΠΈΠΉ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°, совмСстно ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡŽΡ‰ΠΈΡ… ΠΎΡ‚Π»ΠΈΡ‡Π½Ρ‹Π΅ вкусовыС качСства: ΠŸΡ€Π΅ΠΊΡ€Π°ΡΠ½Π°Ρ Π»Π΅Π΄ΠΈ, Оля, ΠšΠ°Π΄Ρ€ΠΈΠ»ΡŒ, Виктория. НовыС Π³ΠΈΠ±Ρ€ΠΈΠ΄Ρ‹ F1 для ΠΏΡ€ΠΎΠΌΡ‹ΡˆΠ»Π΅Π½Π½Ρ‹Ρ… Ρ‚Π΅ΠΏΠ»ΠΈΡ†: Π“950, Π“956, Π“960, ΠœΠ°Π³ΠΈΡΡ‚Ρ€Π°Π»ΡŒ ΠΈ Ρ€ΠΎΠ·ΠΎΠ²ΠΎΠΏΠ»ΠΎΠ΄Π½Ρ‹ΠΉ Π“12897, прСвосходили голландский стандарт ΠΏΠΎ продуктивности Π΄ΠΎ 21%, Π° ΠΏΠΎ вкусовым качСствам Π½Π° 11,8 Π±Π°Π»Π»Π°

    Development of high-lycopene tomato hybrids using conventional breeding techniques and molecular markers

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    Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (Β° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points

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    The value of a new microculture method for diagnosis of visceral leishmaniasis by using bone marrow and peripheral blood

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    PubMedID: 16103589We have demonstrated that the microculture method (MCM) enables the diagnosis of visceral leishmaniasis (VL) with samples from both the bone marrow (BM) and peripheral blood (PB). The MCM is superior to the traditional culture method (TCM) as determined by its higher sensitivity in the detection of promastigotes and the more rapid time for emergence of promastigotes. The sensitivity of MCM (100% in BMs and 77.8-100% in PB) was considerably higher than that of the TCM (37.5-100% in BMs and 0-100% in PB) according to decreasing parasite density (P < 0.05). The concentration of parasites in buffy coats has increased the sensitivity of both methods, especially that of the MCM. Detection of promastigotes by MCM requires lower amounts of culture media (25-50 Β΅L) and shorter incubation periods (2-7 days) than TCM (2.5-3.5 mL and 15-35 days, respectively). MCM was found to be valuable with the advantages of simplicity and sensitivity, in addition to being cost-effective in the routine diagnosis for VL in Adana Turkey. Copyright Β© 2005 by The American Society of Tropical Medicine and Hygiene
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