29 research outputs found
Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΠ»ΠΈΠΊΠΎΠΏΠΈΠ½ΠΎΠ²ΡΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ² ΡΠΎΠΌΠ°ΡΠ° Π΄Π»Ρ ΡΠ΅ΠΏΠ»ΠΈΡ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ²
Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing β Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (Β° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΡΡΠΎΠΊΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
ΡΠΎΠΌΠ°ΡΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ Π²Π°ΠΆΠ½ΡΠΌ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΎΠ½Π½ΡΠΌ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠΌ ΠΏΡΠΈ ΡΠΎΠ·Π΄Π°Π½ΠΈΠΈ Π½ΠΎΠ²ΡΡ
ΡΠΎΡΡΠΎΠ² ΠΈ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ². ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΊ Π°Π»Π»Π΅Π»ΡΠΌ, Π΄Π΅ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΌ Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π· Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°, Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ Ρ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ Π½Π° Π²ΡΡΠΎΠΊΠΎΠ΅ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ²,ΡΠ΅Π½Π½ΡΠ΅ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ²ΠΊΡΡΠΎΠ²ΡΠ΅ΠΊΠ°ΡΠ΅ΡΡΠ²Π°ΠΏΠ»ΠΎΠ΄ΠΎΠ²,Π°ΡΠ°ΠΊΠΆΠ΅ Π½Π° ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡ ΠΊ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ ΠΏΠ°ΡΠΎΠ³Π΅Π½ΠΎΠ², ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΡΠ΅Π½ΠΈΡΡ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ° ΠΈ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΡΠΎΡΡΠ°Π²Π»ΡΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ ΠΏΠΎ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ Π²ΡΡΠΎΠΊΠΎΠ»ΠΈΠΊΠΎΠΏΠΈΠ½ΠΎΠ²ΡΡ
ΡΠΎΡΠΌ Π΄Π»Ρ ΡΡΠ»ΠΎΠ²ΠΈΠΉ Π·Π°ΡΠΈΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π³ΡΡΠ½ΡΠ°.ΠΠ΅ΡΠΎΠ΄ΠΈΠΊΠ°. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡΠΌΠΈ Π°Π»Π»Π΅Π»Π΅ΠΉ, Π΄Π΅ΡΠ΅ΡΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΡ
Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ², ΠΈ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ² ΡΠΎΠΌΠ°ΡΠ° Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΌ ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² β Π²ΡΡΠΎΠΊΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΠΈ ΠΊ Π±ΠΎΠ»Π΅Π·Π½ΡΠΌ, ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΠΈ Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΏΠ»ΠΎΠ΄ΠΎΠ². ΠΠ΅ΡΠΎΠ΄ ΠΈΠ½ΡΡΠΎΠ³ΡΠ΅ΡΡΠΈΠΈ ΡΠΏΠΎΠ½ΡΠ°Π½Π½ΡΡ
ΠΈΠ»ΠΈ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΌΡΡΠ°ΡΠΈΠΉ Π±ΡΠ» ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ Π΄Π»Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΡΠΎΠ²Π½Ρ ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² (og ΠΈ hp) ΠΈ ΡΠ»ΡΡΡΠ΅Π½ΠΈΡ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΊΠ°ΡΠ΅ΡΡΠ² (nor, alc, rin) ΠΏΠ»ΠΎΠ΄ΠΎΠ². ΠΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠ²Π»ΡΠ»ΡΡ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΎΠ½Π½ΡΠΉ, ΠΌΡΡΠ°Π½ΡΠ½ΡΠΉ, ΡΠ΅Π»Π΅ΠΊΡΠΈΠΎΠ½Π½ΡΠΉ, Π³ΠΈΠ±ΡΠΈΠ΄Π½ΡΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» ΡΠΎΠΌΠ°ΡΠ° ΠΈ Π²Π½Π΅ΡΠ΅Π½Π½ΡΠ΅ Π² ΠΠΎΡΡΠ΅Π΅ΡΡΡ Π Π€ Π³ΠΈΠ±ΡΠΈΠ΄Ρ ΡΠΎΠΌΠ°ΡΠ° ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈ Π‘Π‘ ΠΠ³ΡΠΎΡΠΈΡΠΌΡ Β«ΠΠ»ΡΠΈΠ½ΠΈΡΠ½Π°Β» β ΠΠΠΠΠ ΡΠΈΠ»ΠΈΠ°Π» Π€ΠΠΠΠ£ Π€ΠΠ¦Π. ΠΠΠ-ΡΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠ² ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΏΠ»ΠΎΠ΄ΠΎΠ² Π²ΡΠΏΠΎΠ»Π½ΡΠ»ΠΈ Π² ΠΠ½ΡΡΠΈΡΡΡΠ΅ Π³Π΅Π½Π΅ΡΠΈΠΊΠΈ ΠΈ ΡΠΈΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΠΠ ΠΠ΅Π»Π°ΡΡΡΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π‘ΠΎΠ·Π΄Π°Π½Ρ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ Π³ΠΈΠ±ΡΠΈΠ΄Ρ Π΄Π»Ρ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΡ
ΡΠ΅ΠΏΠ»ΠΈΡ Ρ Π²ΡΡΠΎΠΊΠΈΠΌΠΈ Π²ΠΊΡΡΠΎΠ²ΡΠΌΠΈ ΠΈ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²Π°ΠΌΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΠΎΡΡΠ°ΠΏΠ½ΡΡ
ΡΠΊΡΠ΅ΡΠΈΠ²Π°Π½ΠΈΠΉ, ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ²ΡΠΈΡ
ΠΎΠ±ΡΠ΅Π΄ΠΈΠ½ΠΈΡΡ Π³Π΅Π½Ρ nor, rin, alc, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠΈΠ΅ ΠΊ ΡΠ΄Π»ΠΈΠ½Π΅Π½ΠΈΡ ΡΡΠΎΠΊΠΎΠ² Ρ
ΡΠ°Π½Π΅Π½ΠΈΡ Ρ Π³Π΅Π½Π°ΠΌΠΈ B, og, hp1 ΠΈ Π΄Ρ., ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΡΡΠΈΠΌΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΡΠ΅Π»Π΅Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΠΎΠΌ ΠΎΡΠ±ΠΎΡΠ΅ ΠΈ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ, Π½Π΅ΡΠΌΠΎΡΡΡ Π½Π° Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π³Π΅Π½ΠΎΠ² nor, rin, alc Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΠΏΠΎΠ΄Π½ΡΡΡ ΡΡΠΎΠ²Π΅Π½Ρ ΠΊΠ°ΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² Π΄ΠΎ ΡΡΠ΅Π΄Π½ΠΈΡ
Π²Π΅Π»ΠΈΡΠΈΠ½. ΠΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΡ ΡΡΠΎΠ²Π½Ρ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Π² ΠΏΠ»ΠΎΠ΄Π°Ρ
Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ ΠΈ ΡΡΠΎΠ²Π½Ρ ΠΈΠ½ΡΠΎΠ»ΡΡΠΈΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΠΎΠ·ΠΎΠ²ΠΎΠΏΠ»ΠΎΠ΄Π½ΡΠ΅ ΡΠΎΡΠΌΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Ρ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π±ΠΎΠ»ΡΡΠ΅Π΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°, ΡΠ΅ΠΌ ΠΊΡΠ°ΡΠ½ΠΎΠΏΠ»ΠΎΠ΄Π½ΡΠ΅. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Ρ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΡ Π°Π»Π»Π΅Π»Π΅ΠΉ ΡΡΡΡΠΊΡΡΡΠ½ΡΡ
Π³Π΅Π½ΠΎΠ² Π±ΠΈΠΎΡΠΈΠ½ΡΠ΅Π·Π° ΠΊΠΎΡΠΎΡΠΈΠ½ΠΎΠΈΠ΄ΠΎΠ² ΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ
Π³Π΅Π½ΠΎΠ², ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΡ
ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠ΅ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΠ΅ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π° Ρ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ² Ρ ΠΊΡΠ°ΡΠ½ΡΠΌΠΈ ΠΈ ΡΠΎΠ·ΠΎΠ²ΡΠΌΠΈ ΠΏΠ»ΠΎΠ΄Π°ΠΌΠΈ, ΡΠΎΡΠΌΡΠ»Ρ ΠΊΠΎΡΠΎΡΡΡ
ΡΠ°Π·Π»ΠΈΡΠ°Π»ΠΈΡΡ ΠΏΠΎ ΡΠΎΡΡΠ°Π²Ρ Π°Π»Π»Π΅Π»Π΅ΠΉ Π³Π΅Π½ΠΎΠ² ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΏΠ»ΠΎΠ΄ΠΎΠ². ΠΡΠ΄Π΅Π»Π΅Π½Ρ Π³ΠΈΠ±ΡΠΈΠ΄Ρ Ρ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠ΅ΠΌ Π²ΡΡΠΎΠΊΠΈΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ ΡΠ°Ρ
Π°ΡΠ° (Β°Brix), ΡΡΡ
ΠΎΠ³ΠΎ Π²Π΅ΡΠ΅ΡΡΠ²Π° ΠΈ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ
Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ Π»ΠΈΠΊΠΎΠΏΠΈΠ½Π°, ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡΠΈΡ
ΠΎΡΠ»ΠΈΡΠ½ΡΠ΅ Π²ΠΊΡΡΠΎΠ²ΡΠ΅ ΠΊΠ°ΡΠ΅ΡΡΠ²Π°: ΠΡΠ΅ΠΊΡΠ°ΡΠ½Π°Ρ Π»Π΅Π΄ΠΈ, ΠΠ»Ρ, ΠΠ°Π΄ΡΠΈΠ»Ρ, ΠΠΈΠΊΡΠΎΡΠΈΡ. ΠΠΎΠ²ΡΠ΅ Π³ΠΈΠ±ΡΠΈΠ΄Ρ F1 Π΄Π»Ρ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΡ
ΡΠ΅ΠΏΠ»ΠΈΡ: Π950, Π956, Π960, ΠΠ°Π³ΠΈΡΡΡΠ°Π»Ρ ΠΈ ΡΠΎΠ·ΠΎΠ²ΠΎΠΏΠ»ΠΎΠ΄Π½ΡΠΉ Π12897, ΠΏΡΠ΅Π²ΠΎΡΡ
ΠΎΠ΄ΠΈΠ»ΠΈ Π³ΠΎΠ»Π»Π°Π½Π΄ΡΠΊΠΈΠΉ ΡΡΠ°Π½Π΄Π°ΡΡ ΠΏΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π΄ΠΎ 21%, Π° ΠΏΠΎ Π²ΠΊΡΡΠΎΠ²ΡΠΌ ΠΊΠ°ΡΠ΅ΡΡΠ²Π°ΠΌ Π½Π° 11,8 Π±Π°Π»Π»Π°
Development of high-lycopene tomato hybrids using conventional breeding techniques and molecular markers
Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing β Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (Β° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points
The value of a new microculture method for diagnosis of visceral leishmaniasis by using bone marrow and peripheral blood
PubMedID: 16103589We have demonstrated that the microculture method (MCM) enables the diagnosis of visceral leishmaniasis (VL) with samples from both the bone marrow (BM) and peripheral blood (PB). The MCM is superior to the traditional culture method (TCM) as determined by its higher sensitivity in the detection of promastigotes and the more rapid time for emergence of promastigotes. The sensitivity of MCM (100% in BMs and 77.8-100% in PB) was considerably higher than that of the TCM (37.5-100% in BMs and 0-100% in PB) according to decreasing parasite density (P < 0.05). The concentration of parasites in buffy coats has increased the sensitivity of both methods, especially that of the MCM. Detection of promastigotes by MCM requires lower amounts of culture media (25-50 Β΅L) and shorter incubation periods (2-7 days) than TCM (2.5-3.5 mL and 15-35 days, respectively). MCM was found to be valuable with the advantages of simplicity and sensitivity, in addition to being cost-effective in the routine diagnosis for VL in Adana Turkey. Copyright Β© 2005 by The American Society of Tropical Medicine and Hygiene