Reduced Calcium Channel Function in the Drosophila cacTS2 mutant on Vision, Olfaction and Regulation of the Hear

The cacTS2 Drosophila line have reduced Ca2+ channel function at raised temperatures. The inhibition of calcium to flow inside the neuron causes a reduction in synaptic transmission at the neuromuscular junction. When the adults of cacTS2 are exposed to 38°C for a few minutes they rapidly stop flying and walking which implies the alterations measured electrophysiologically in the skeletal muscles of larvae also apply to motor units in skeletal muscles of adults as well as to possibly other sites of depressed synaptic communication. Here we used the cacTS2 strain to investigate the potential actions in vision and olfaction in adult Drosophila. Where as in larval Drosophila, we further assess sensory regulation of heart rate as well as direct action in the heart at restrictive temperatures to further delineate the properties of the cacTS2 strain. Mutations that are temperature sensitive provide a good means to study the mechanisms of regulating homeostasis over time. Since clinically the regulation of calcium channel function in conditions of epilepsy and convulsions are used, the long-term effect of reducing calcium channel function is of interest. We will report on the effects of varied heat pulses during formation of the adult CNS for the cacTS2 and CS lines

La dacryocystorhinostomie par voie endo-nasale sans unciformectomie

POITIERS-BU Médecine pharmacie (861942103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

The Effects of CO2 on Drosophila Larvae: Possible Neural Components

Adult insects have been shown to have sensory structures that detect carbon dioxide (CO2) which can direct insects toward food sources. However, too high CO2 is anesthetic to insects. No prior studies have reported carbon dioxide sensory neurons in Drosophila larva. Previous experiments supposed that carbon dioxide affected larvae in the same way that it affects humans: an increase in body fluid acidity causing different behaviors, including anesthesia. We show that cardiac activity, body wall locomotion and mouth hook movement cease in less than a minute and recovery in less than a minute. A pure N2 environment does not elicit these responses even over 10 minutes. Such rapid changes caused by CO2 strongly suggesting a neural response. We are now examining where the potential CO2 receptors are located on the animal. The objective of this current research is to find sensory neurons on the larvae capable of detecting the CO2. Various sensory nerve roots are being monitored in a semi-intact preparations for electrical activity induced by CO2 exposure