Regulation of the neuronal proteasome by Zif268 (Egr1)

Most forms of neuronal plasticity are associated with induction of the transcription factor Zif268 (Egr1/Krox24/NGF-IA). In a genomewide scan, we obtained evidence for potential modulation of proteasome subunit and regulatory genes by Zif268 in neurons, a finding of significance considering emerging evidence that the proteasome modulates synaptic function. Bioinformatic analysis indicated that the candidate proteasome Zif268 target genes had a rich concentration of putative Zif268 binding sites immediately upstream of the transcriptional start sites. Regulation of the mRNAs encoding the psmb9 (Lmp2) and psme2 (PA28�) proteasome subunits, along with the proteasome-regulatory kinase serum/glucocorticoid-regulated kinase (SGK) and the proteasome-associated antigen peptide transporter subunit 1 (Tap1), was confirmed after transfection of a neuronal cell line with Zif268. Conversely, these mRNAs were upregulated in cerebral cortex tissue from Zif268 knock-out mice relative to controls, confirming that Zif268 suppresses their expression in the CNS. Transfected Zif268 reduced the activity of psmb9, SGK, and Tap1 promoter–reporter constructs. Altered psmb9, SGK, and Tap1 mRNA levels were also observed in an in vivo model of neuronal plasticity involving Zif268 induction: the effect of haloperidol administration on striatal gene expression. Consistent with these effects on proteasome gene expression, increased Zif268 expression suppressed proteasome activity, whereas Zif268 knock-out mice exhibited elevated cortical proteasome activity. Our findings reveal that Zif268 regulates the expression of proteasome and related genes in neuronal cells and provide new evidence that altered expression of proteasome activity after Zif268 induction may be a key component of long-lasting CNS plasticity

Characterizing phosphorus forms in Saskatchewan soils using solution 31P NMR spectroscopy

Non-Peer ReviewedIn soils and other environmental samples, phosphorus (P) may be found in a range of inorganic and organic forms. Inorganic P forms include orthophosphate, which is readily available to plants, and complex inorganic P forms such as pyrophosphate and polyphosphate. Organic P forms can be divided into groups such as orthophosphate monoesters (e.g. sugar phosphates, phytic acid) and orthophosphate diesters (e.g. phospholipids, DNA) and phosphonates. Identifying P forms is important to enhance crop growth, with and without fertilization, and to minimize P loss to water, where it can cause harmful algal blooms. This presentation describes some of our recent research using solution 31P NMR spectroscopy to characterize P forms in a range of Saskatchewan soils, as well as swine manure

Wavelength dependent collective effects in the multiphoton ionization of atomic deuterium

This paper presents the results of an experimental investigation into collective effects in the transient plasma formed by multiphoton ionization of atomic deuterium with a pulsed laser. The laser wavelength is varied in a narrow range around 243 nm, so that the photoionization is resonant with the metastable 2S1/2 state. The ion yield, the ion time-of-flight spectra, and the yield of Lyman-a photons have been measured as a function of laser intensity ~from 1 to 340 MW/cm2! and laser detuning around the 1S1/2-2S1/2 two-photon resonance. During and shortly after the laser pulse, collective effects resulting from the mutual interaction of the photoelectrons and the ions affect the spatial and temporal distribution of the ions. Because of the near-degeneracy of the 2S1/2, 2P1/2 , and 2P3/2 states, the resonant multiphoton ionization is affected by the Stark mixing of these states in the collective field. As a result, the time-dependent yields of ions and of Lyman-a photons are modulated by the interplay of the multiphoton ionization of the atoms and the collective effects in the plasma. From the measurements it is deduced that collective effects are important above a critical charge density of 33108 ions/cm3. An asymmetry is observed in the line profile of the total ion yield as a function of laser detuning. This asymmetry is interpreted to be due to the effect of the collective field upon the intermediate resonant 2S1/2 state of the photoionization process

Vortex Matter in Mesoscopic Superconducting Disks and Rings

Phase transitions between different (i.e. giant and multi-vortex) superconducting states and between the superconducting-normal state of mesoscopic disks and rings are studied in the presence of an external magnetic field by solving the two non-linear Ginzburg-Landau equations self-consistently. The flux through a circular disk with a hole in the middle is not quantized.Comment: 8 pages, 10 figures; to appear in Physica C (proceedings of the conference on Vortex matter, Crete (september 1999

Wavelength dependent collective effects in the multiphoton ionization of atomic deuterium

This paper presents the results of an experimental investigation into collective effects in the transient plasma formed by multiphoton ionization of atomic deuterium with a pulsed laser. The laser wavelength is varied in a narrow range around 243 nm, so that the photoionization is resonant with the metastable 2S1/2 state. The ion yield, the ion time-of-flight spectra, and the yield of Lyman-a photons have been measured as a function of laser intensity ~from 1 to 340 MW/cm2! and laser detuning around the 1S1/2-2S1/2 two-photon resonance. During and shortly after the laser pulse, collective effects resulting from the mutual interaction of the photoelectrons and the ions affect the spatial and temporal distribution of the ions. Because of the near-degeneracy of the 2S1/2, 2P1/2 , and 2P3/2 states, the resonant multiphoton ionization is affected by the Stark mixing of these states in the collective field. As a result, the time-dependent yields of ions and of Lyman-a photons are modulated by the interplay of the multiphoton ionization of the atoms and the collective effects in the plasma. From the measurements it is deduced that collective effects are important above a critical charge density of 33108 ions/cm3. An asymmetry is observed in the line profile of the total ion yield as a function of laser detuning. This asymmetry is interpreted to be due to the effect of the collective field upon the intermediate resonant 2S1/2 state of the photoionization process

Restricted three body problems at the nanoscale

In this paper, we investigate some of the classical restricted three body problems at the nanoscale, such as the circular planar restricted problem for three C60 fullerenes, and a carbon atom and two C60 fullerenes. We model the van der Waals forces between the fullerenes by the Lennard-Jones potential. In particular, the pairwise potential energies between the carbon atoms on the fullerenes are approximated by the continuous approach, so that the total molecular energy between two fullerenes can be determined analytically. Since we assume that such interactions between the molecules occur at sufficiently large distance, the classical three body problems analysis is legitimate to determine the collective angular velocity of the two and three C60 fullerenes at the nanoscale. We find that the maximum angular frequency of the two and three fullerenes systems reach the terahertz range and we determine the stationary points and the points which have maximum velocity for the carbon atom for the carbon atom and the two fullerenes system

Flux transitions in a superconducting ring

We perform a numeric study of the flux transitions in a superconducting ring at fixed temperature, while the applied field is swept at an ideally slow rate. The current around the ring and its free energy are evaluated. We partially explain some of the known experimental features, and predict a considerably large new feature: in the vicinity of a critical field, giant jumps are expected

Competition between electronic cooling and Andreev dissipation in a superconducting micro-cooler

We discuss very low temperature experiments on superconducting micro-coolers made of a double Normal metal - Insulator - Superconductor junction. We investigate with a high resolution the differential conductance of the micro-cooler as well as of additional probe junctions. There is an explicit crossover between the single quasi-particle current and the phase-coherent Andreev current. We establish a thermal model by considering the thermal contribution due to the Andreev current. The related increase of the electron temperature is discussed, including the influence of several parameters like the phase-coherence length or the tunnel junction transparency