ТАНДАРТНЫЕ ОБРАЗЦЫ В ОБЛАСТИ ИСПОЛЬЗОВАНИЯ АТОМНОЙ ЭНЕРГИИ

The article describes the chronology of development of the system of reference materials in the nuclear industry of the Russian Federation. The basic documents used in the sphere of nuclear energy are described. The nomenclature of reference materials and feature of their application in the "Rosatom" is given. The prospects of development activities in the field of reference materials are formulated.В данной статье описана хронология развития системы стандартных образцов в атомной отрасли Российской Федерации. Описаны основные нормативные документы, применяемые в области использования атомной энергии, номенклатура стандартных образцов и особенность их применения в Госкорпорации «Росатом», проанализированы подходы к их аттестации. Сформулированы перспективы развития деятельности в области стандартных образцов

REFERENCE MATERIALS IN THE SPHERE OF USE OF ATOMIC ENERGY

The article describes the chronology of development of the system of reference materials in the nuclear industry of the Russian Federation. The basic documents used in the sphere of nuclear energy are described. The nomenclature of reference materials and feature of their application in the "Rosatom" is given. The prospects of development activities in the field of reference materials are formulated

TREATMENT OUTCOMES OF THE ALVEOLAR RIDGE REGRESSIVE TRANSFORMATION USING AUTOLOGOUS ADIPOSE-TISSUE DERIVED STROMAL VASCULAR FRACTION

Aim. The study was performed in order to assess the size, structure and chewing ability of the maxillary ridge reconstructed using autologous adipose-tissue derived stromal vascular fraction (ATD SVF) in a long-term study (10 years).Materials and methods. The study involved 141 patients (61 men, 80 women) aged from 45 to 78 years (mean age 57 years) with a regression transformation of the alveolar ridge of the upper and lower jaw. In the test group (TG), 112 osteoplastic surgeries were performed using autologous adipose-tissue derived stromal vascular fraction (ATD SVF) followed by the installation of 297 dental implants into the reconstructed ridge to restore the chewing function. The control group (CG) comprised 117 surgeries performed according to generally accepted methods followed by the installation of 323 artifi cial supports. The size of the alveolar ridge and the support function of the reconstructed bone were evaluated during the period of up to 10 years. In the histological and histomorphometric study, 27 trephine biopsy specimens of reconstructed bone obtained during dental implantation were studied. The resulting digital material was processed by conventional methods of variation statistics.Results. The results of a comparative study have confi rmed the advantages of the proposed approach with the use of ATD SVF over current generally accepted methods for the reconstruction of the jaw alveolar ridge. The use of ATD SVF in the composition of osteoplastic material allows the required degree of augmentation of the atrophied bone area to be achieved with a minimal risk of complications and reoperations (8% and 21% of cases in TG and CG, respectively, p = 0.231), along with the optimal morphological characteristics of the regenerate (40.14 ± 3.36 and 24.23 ± 2.63 percent of viable mineralized bone tissue on sections of trephine biopsy specimens from TG and KG, p = 0.001). These results ensure reliable osseointegration of artifi cial supports in the reconstructed alveolar ridge and a high effi ciency of orthopedic treatment with implant supports during the period of up to 10 years (97% and 88% in TG and CG, respectively, r˂0.001).Conclusion. The proposed innovative approach can be recommended as a basis for a surgical protocol with a pronounced regression transformation of the jaw alveolar ridge. This will allow the chewing function to be restored more successfully and predictably with the use of artifi cial supports in the reconstructed bone in this category of patients

THE USE OF PREDICTIVE CYTOLOGICAL FEATURES FOR THE SELECTION OF THE METHODOLOGY OF REGENERATIVE TREATMENT OF PATIENTS WITH PERIODONTITIS

Aim. This study was conducted to assess the outcome of the results obtained in the treatment of patients with longterm current periodontitis with individual characteristics of the cytological picture of the microenvironment of damaged periodontal tissues.Materials and methods. The main study group included 40 patients with chronical periodontitis of the moderate degree (Mo 56, 7 years). The selection of the regenerative treatment in the main group was carried out in accordance with the preliminary express assessment of the cytological picture of the damaged periodontal tissues. The control group, whose indicators were compared with the main clinical indicators, included 43 patients with randomly selected regenerative treatment.Results. The cell-potential surgical approach statistically induces the growth of new dentogingival junction in patients with critically small amount of morphologically altered neutrophil granulocytes and small cell forms with intense basophilic coloring (≤20%) in cytological samples in comparison with the control decreasing the amount of residual loss of teeth supporting tissues by 2 times.Conclusion. The preliminary express assessment of the cytological picture of the microenvironment of periodontal pockets indirectly indicates the potential of the patient with periodontitis to restore the missing structures of the periodontal membrane. The choice of the surgical tactics of regenerative treatment, taking into account the characteristics of cellular behavior in cytological samples of patients with periodontitis, is advisable to use as a prognostic test to improve the final results

EVALUATION OF THE EFFICIENCY OF THE AUTOTRANSPLANTATION OF THE ENRICHED WITH VASCULAR STROMAL CELL FRACTION ADIPOSE TISSUE IN CONTOUR PLASTIC SURGERY OF THE FACIAL SOFT TISSUE

The results of adipose tissue autotransplantation in patients with different etiology of local facial soft tissue deficiency were estimated. The basic group (BG, n=105) was treated with vascular stromal cell fraction (VSCF) enriched fat, and control group (CG, n=98) with pure fat. The better outcomes after unitary operation (2,0±0,1 against 1,4±0,1 according to GAIS ratings;

RESULTS OF CELL-POTENTIATED SURGICAL TREATMENT OF CHRONIC PERIODONTITIS (10 years of observations)

Aim. The aim of this study is to systematize the scientific knowledge and reconstructed periodontium reliability degree by means of cell-potentiated surgical treatment of patients with the chronic periodontitis.Materials and methods. All 76 patients included in the study (aged 23 to 68 years, Mo 55.3 years) underwent a surgery for directed regeneration of periodontal tissues which was supplemented by transplantation of vascular stromal cell fraction isolated from the patient's adipose tissue (VSF AT). The evaluation of the results of the main periodontal parameters, processed by means of variation statistics in 690 periodontal defects of different topography, was carried out within 10 years.Results. The use of the VSF AT demonstrated the advantage of the method expressed by the formation of a new timeresistant dentogingival junction with the reconstruction of the alveolar bone with a minimum amount of the marginal recession and the probing depth.Conclusion. The percentage of positive results in the representative statistical sample was 90.3% in the main group, 75.6% in the control group within 10 years

Supernumerary (B) chromosomes in populations of Picea abies (L.) H. Karst. from Western Rhodopes (Bulgaria)

Investigations on B chromosomes found for the first time for Picea abies (L.) H. Karst. have been conducted. Seeds of Picea abies from two populations of Western Rhodopes (Bulgaria) located at the southern border of species range, and protected according to Bern Convention and EC Habitat Directive were collected for this study. Mixoploidy was detected in some germinating seeds of Picea abies. It was found that metaphase cells of germinating seeds contain 0–4 B chromosomes of both metacentric and submetacentric types. The variability of B chromosomes number and their occurrence was observed. Along with B chromosomes, some chromosome aberrations such as fragments and ring chromosomes were revealed in metaphase cells of Picea abies from studied populations. The possible adaptive role of B chromosomes presence for Picea spp. is discussed.Проведено исследование В-хромосом, обнаруженных впервые у вида Picea abies (L.) H. Karst. Для настоящего исследования семена собраны из двух популяций Picea abies в Западных Родопах (Болгария), расположенных на южной границе видового ареала и охраняемых в соответствии с Бернской конвенцией и Директивой ЕС о местообитаниях. В отдельных проростках семян Picea abies найдена миксоплоидия. Обнаружено, что метафазные клетки проростков семян из изученных популяций Picea abies содержат 0–4 В-хромосомы как метацентрического, так и субметацентрического типов. Наблюдается изменчивость числа В-хромосом и их появления. Наряду с В-хромосомами в метафазных клетках Picea abies выявлены хромосомные мутации – фрагменты и кольцевые хромосомы. Обсуждается возможная адаптивная роль присутствия В-хромосом у видов Picea.Проведено дослідження В-хромосом, виявлених вперше у виду Picea abies (L.) H. Karst. Для даного дослідження насіння зібране з двох популяцій Picea abies в Західних Родопах (Болгария), що розташовані на південному кордоні видового ареалу та охороняються відповідно до Бернської конвенції та Директивою ЄС про місце розповсюдження. В окремих проростках насіння Picea abies знайдено міксоплоїдію. Виявлено, що метафазні клітини проростків насіння з вивчених популяцій Picea abies містять 0–4 В-хромосоми як метацентричного, так і субметацентричного типів. Спостерігається мінливість числа В-хромосом. Поряд з В-хромосомами у метафазних клітинах Picea abies виявлено хромосомні мутації – фрагменти та кільцеві хромосоми. Обговорюється можлива адаптивна роль присутності В-хромосом у видів Picea