A STABILITY-INDICATING AND VALIDATED REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PHENYLEPHRINE AND FEXOFENADINE IN BULK AND TABLET DOSAGE FORMS

ABSTRACTObjective: A simple and accurate stability indicating reversed-phase high-performance liquid chromatography method was developed and validatedfor the simultaneous estimation of phenylephrine and fexofenadine in pharmaceutical dosage forms.Methods: Chromatography was carried out on an ODS C-18 column (4.6 mm×250 mm, 5 µ particle size) with a isocratic mobile phase composed oforthophosphoric acid buffer, acetonitrile, (75:25% v/v) at a flow rate of 1 mL/minutes. The column temperature was maintained at 30°C and thedetection was carried out using a photodiode array detector at 210 nm.Results: The retention times for phenylephrine and fexofenadine were 2.096 minutes and 4.241 minutes, respectively. The percentage recoveriesof phenylephrine and fexofenadine were 100.63% and 99.84%, respectively. The relative standard deviation for assay of tablets was found to be<2%. The detection and quantification limits were found to be 0.10 and 0.31 µg/mL for phenylephrine and 0.01 and 0.03 µg/mL for fexofenadine,respectively.Conclusion: Thus, the method was fast, accurate, precise, and sensitive; hence, it can be employed for routine quality control of tablets containingboth drugs in quality control laboratories and pharmaceutical industries.Keywords: Phenylephrine, Fexofenadine, Stability indicating method, Validation method, Reversed-phase high performance liquid chromatography

Time stamped Digital Signature Scheme with Message Recovery & Its Application in E-Cash System

We propose a Timestamped signature scheme which can be verified universally using signer’s public parameters. A trusted third party, the Timestamping System provides timestamp to a signature without even knowing the content of the document. The proposed scheme can withstand active attacks, such as forgery attack and chosen cipher text attack. It also provides the message recovery feature, i.e., from the timestamped signature, the message can be recovered by the receiver. Hence, the message need not be sent with the signature. The suggested scheme do not require any hash function and there by reduces the verification cost as compared to existing schemes at the expense of marginal increase in signature generation cost. Further, the scheme is more secured as its security lies in solving three computationally hard assumptions Performance analysis of both the schemes has been carried out in details. We applied the Time-stamped signature scheme with Message recovery in Ecash syste

EFFECT OF EFFERVESCENCE IN COMBINATION WITH SUPERDISINTEGRANTS IN THE FORMULATION OF PROPRANOLOL HCL ORAL DISINTEGRATING TABLETS

ABSTRACTObjective: The current research work is intended to formulate propranolol HCl (PLH) as orally disintegrating tablet (ODT). It is also intending to checkthe superiority in a combination of superdisintegrants and effervescent mixture than the use of superdisintegrants alone by a direct compressiontechnique. To fasten the onset of action and thereby enhancing the bioavailability of PLH in comparison to its conventional tablets.Methods: Standard calibration curve of PLH was obtained in pH 6.8 phosphate buffer by spectrophotometric method, drug-excipient compatibilitystudies were carried by Fourier transform infrared (FT-IR) studies. All the formulations were evaluated for pre and postcompression studies.Accelerated stability studies were carried out up to 6 months for the optimized formulation, EF3.Results and Discussion: Superdisintegrants used in the study are compatible with PLH. Pre- and post-compression parameters were within theacceptable limits for all formulations. In vitro dissolution kinetic studies indicate the release of PLH from ODT increases as the concentration ofsuperdisintegrants as well as the ratio of citric acid: NaHCO3 of effervescent mixture increases. Formulations with an effervescent mixture are havingrapid disintegration and dissolution rate when compared to the formulations with superdisintegrants alone. The order of superdisintegrants inenhancing the dissolution rate of PLH is crospovidone (CPV) > croscarmellose sodium (CCS) > sodium starch glycolate (SSG). Formulation, EF3 (10%CPV and 1:3, citric acid: NaHCO3 ratio, respectively) had the highest dissolution efficiency at 10 minutes (DE10=82.74%); the first order dissolutionrate constant (K1=0.141/minutes) with a regression coefficient (r2=0.974) and lesser time for 90% of drug release (t90=4 minutes), was considered asthe optimal ODT in this study. Formulation EF3, passed the test for stability.Conclusion: Hence, an effective PLH ODT was formulated by the direct compression technique with disintegration by combination of superdisintegrantsand effervescent mixture, will fasten the onset of action and enhances the bioavailability of PLH in comparison to its conventional tablets.Keywords: Propranolol HCl, Orally disintegrating tablet, Sodium starch glycolate, Croscarmellose sodium, Crospovidone, Direct compression, In vitrodissolution studies

Development and validation of new analytical method for the simultaneous estimation of omeprazole and domperidone in pharmaceutical dosage form by UV spectrophotometry

A simple, rapid and precise method was developed for the quantitative simultaneous determination of Omeprazole and Domperidone in combined pharmaceutical-dosage forms. The method was based on UV-Spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 291 nm (λmax of Omeprazole) and 289 nm (λmax of Domperidone) in Methanol: Acetonitrile (30:70 v/v). For UV Spectrophotometric method, linearity was obtained in concentration range of 1-15 µg/ml for Domperidone and 1-50 µg/ml for Omeprazole respectively, with regression 0.999 and 0.999 for Domperidone and Omeprazole respectively. Recovery was in the range of 99 -103%; the value of standard deviation and %R.S.D were found to be < 2 %; shows the high precision of the method., in accordance with ICH guidelines. The method has been successively applied to pharmaceutical formulation and was validated according to ICH guidelines

Deepfake Detection and Reconstruction of the Original Image

This study takes a novel method to addressing the problems faced by false faces created by adversarial networks. The system includes a Fake Face Identification Module and a Generative Image Reconstruction Module that use deep learning techniques. The former correctly recognises falsely manufactured faces, but the latter reconstructs the original images associated with the discovered forgeries. . The procedure entails training advanced deep neural networks on a variety of datasets in order to ensure flexibility against evolving adversarial tactics. The technology improves biometric security, data integrity, and the trustworthiness of facial recognition systems by cross-referencing and recovering legitimate photos in datasets. Identity verification, surveillance, and the preservation of accurate facial picture databases are all potential applications for this projec

FLEXIBLE DATA STORAGE, RUGGEDLY BEDECKED AND BRILLIANT IN CLOUD COMPUTING

Within the prevailing aim, every time an enjoyer leaves on the purchaser class, the target market boss simplest revokes his club underground key that means the purchaser’s inner most key related attributes remains weighty. Our project is acceptable for refuge blocked devices. If an individual in the categorize deliberately exposes the target market confidential information obey the revoked shopper, he's able to carry out figuring out operations past his inner most key. To provide an explanation for this person blast, a congealed mention is accessible. We end up the security in our plot covered the divided ciphering Diffie-Hellman (DCDH) acceptance. Regrettably, ABE design calls for expensive data processing expense during carry outing rasp encryption and working out operations. This apostatize distract be massed harsh for incompetent devices as a result of their secured computing sources. Within the thing indicated practice, we consider cunning a Club penguin-ABE arrange beside useful customer cancellation for muddy argosy artifice. Caused by our examination shows guess expense for local devices is relatively low and will be attached. We attempt to design plot blast browned by revoked purchasers cooperating alongside current shoppers. In extension, we formulate an efficient shopper voiding Club penguin-ABE idea by means of accentuating the present work out and end up our design is CPA reliable nether the selective variety