Design integration and noise studies for jet STOL aircraft. Task 7A: Augmentor wing cruise blowing valveless system. Volume 1: System design and test integration

Exploratory design studies conducted to establish the configuration of an augmentor wing vruise blowing (valveless) system in a 150-passenger STOL airplane were reported in NASA CR-114570. Those studies have been updated to incorporate the results of static rig, flow duct, and wind tunnel tests. Minor adjustments in duct flow velocity, flap length, and blowing nozzle geometry were incorporated to provide airplane characteristics that minimize takeoff gross weight and achieve sideline noise objectives for an advanced commercial STOL airplane

Performance of the 19XB 10-Stage Axial-Flow Compressor with Altered Blade Angles

Previous performance data of the 19XB axial-flow compressor indicated that the outlet guide vanes and possibly the inlet guide vanes were stalling. Calculations were made to determine if these adverse conditions could be eliminated and if the manufacturer's design specifications could be more nearly approached by altering the blade angles of the first few compression stages as well as the outlet guide vanes. With the blade angles altered, experimental data were taken at compressor speeds of 8500 to 17,000 rpm with inlet-air conditions of 7.4 inches of mercury absolute and 59 0 F. The temperature-rise efficiency increased with speed from 0.70 at 8500 rpm to 0.74 at 13,600 rpm and dropped gradually to 0.70 at 17,000 rpm. At the design speed of 17,000 rpm, the pressure ratio at the peak efficiency point was 3.63. The maximum pressure ratio at design speed was 4.15 at an equivalent weight flow of 29.8 pounds per second. The altered compressor operated very .near the design specifications of pressure ratio and equivalent weight flow. At the high speeds, the peak adiabatic temperature-rise efficiency was increased 0.02 to 0,06 by altering the blade angles. The peak pressure ratio was increased 0.29 at design speed (17,000 rpm) and 0.05 and 0.13 at 11,900 and 13,600 rpm, respectively. The equivalent weight flow through the altered compressor was reduced 2 pounds per second at 15,300 and 17,000 rpm, as was expected from the design calculations. As extreme caution was taken not to surge the compressor violently, the point of minimum air flow may not have been reached in the present investigation and in a previous investigation. A true comparison of the pressure ratios obtained at the high speeds therefore cannot be made

MACAT—microarray chromosome analysis tool

By linking differential gene expression to the chromosomal localization of genes, one can investigate microarray data for characteristic patterns of expression phenomena involving sizeable parts of specific chromosomes. We have implemented a statistical approach for identifying significantly differentially expressed chromosome regions. We demonstrate the applicability of the approach on a publicly available data set on acute lymphocytic leukemia

DLocalMotif: a discriminative approach for discovering local motifs in protein sequences

Motivation: Local motifs are patterns of DNA or protein sequences that occur within a sequence interval relative to a biologically defined anchor or landmark. Current protein motif discovery methods do not adequately consider such constraints to identify biologically significant motifs that are only weakly over-represented but spatially confined. Using negatives, i.e. sequences known to not contain a local motif, can further increase the specificity of their discovery

Вопросы проектирования эффективных СФЗ

In order to screen for differentially expressed genes that might be useful in diagnosis or therapy of prostate cancer we have used a custom made Affymetrix GeneChip containing 3950 cDNA fragments. Expression profiles were obtained from 42 matched pairs of mRNAs isolated from microdissected malignant and benign prostate tissues. Applying three different bioinformatic approaches to define differential gene expression, we found 277 differentially expressed genes, of which 98 were identified by all three methods. Fourteen per cent of these genes were not found in other expression studies, which were based on bulk tissue. Resultant candidate genes were further validated by quantitative RT-PCR, mRNA in situ hybridization and immunohistochemistry. AGR2 was over-expressed in 89% of prostate carcinomas, but did not have prognostic significance. Immunohistologically detected over-expression of MEMD and CD24 was identified in 86% and 38.5% of prostate carcinomas respectively, and both were predictive of PSA relapse. Combined marker analysis using MEMD and CD24 expression proved to be an independent prognostic factor (RR = 4.7, p = 0.006) in a Cox regression model, and was also superior to conventional markers. This combination of molecular markers thus appears to allow improved prediction of patient prognosis, but should be validated in larger studies. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd

A Novel Putative miRNA Target Enhancer Signal

It is known that miRNA target sites are very short and the effect of miRNA-target site interaction alone appears as being unspecific. Recent experiments suggest further context signals involved in miRNA target site recognition and regulation. Here, we present a novel GC-rich RNA motif downstream of experimentally supported miRNA target sites in human mRNAs with no similarity to previously reported functional motifs. We demonstrate that the novel motif can be found in at least one third of all transcripts regulated by miRNAs. Furthermore, we show that motif occurrence and the frequency of miRNA target sites as well as the stability of their duplex structures correlate. The finding, that the novel motif is significantly associated with miRNA target sites, suggests a functional role of the motif in miRNA target site biology. Beyond, the novel motif has the impact to improve prediction of miRNA target sites significantly

Sex-specific pathways in early cardiac response to pressure overload in mice

Pressure overload (PO) first causes cardiac hypertrophy and then heart failure (HF), which are associated with sex differences in cardiac morphology and function. We aimed to identify genes that may cause HF-related sex differences. We used a transverse aortic constriction (TAC) mouse model leading to hypertrophy without sex differences in cardiac function after 2 weeks, but with sex differences in hypertrophy 6 and 9 weeks after TAC. Cardiac gene expression was analyzed 2 weeks after surgery. Deregulated genes were classified into functional gene ontology (GO) categories and used for pathway analysis. Classical marker genes of hypertrophy were similarly upregulated in both sexes (α-actin, ANP, BNP, CTGF). Thirty-five genes controlling mitochondrial function (PGC-1, cytochrome oxidase, carnitine palmitoyl transferase, acyl-CoA dehydrogenase, pyruvate dehydrogenase kinase) had lower expression in males compared to females after TAC. Genes encoding ribosomal proteins and genes associated with extracellular matrix remodeling exhibited relative higher expression in males (collagen 3, matrix metalloproteinase 2, TIMP2, and TGFβ2, all about twofold) after TAC. We confirmed 87% of the gene expression by real-time polymerase chain reaction. By GO classification, female-specific genes were related to mitochondria and metabolism and males to matrix and biosynthesis. Promoter studies confirmed the upregulation of PGC-1 by E2. Less downregulation of metabolic genes in female hearts and increased protein synthesis capacity and deregulation of matrix remodeling in male hearts characterize the sex-specific early response to PO. These differences could contribute to subsequent sex differences in cardiac function and HF

NACA Research Memorandums

"Previous performance data of the 19XB axial-flow compressor indicated that the outlet guide vanes and possibly the inlet guide vanes were stalling. Calculations were made to determine if these adverse conditions could be eliminated and if the manufacturer's design specifications could be more nearly approached by altering the blade angles of the first few compression stages as well as the outlet guide vanes. With the blade angles altered, experimental data were taken at compressor speeds of 8500 to 17,000 rpm with inlet-air conditions of 7.4 inches of mercury absolute and 59 degrees Fahrenheit" (p. 1)