3 research outputs found
Atomic Force Microscopy of height fluctuations of fibroblast cells
We investigated the nanometer scale height fluctuations of 3T3 fibroblast
cells with the atomic force microscope (AFM) under physiological conditions.
Correlation between these fluctuations and lateral cellular motility can be
observed. Fluctuations measured on leading edges appear to be predominantly
related to actin polymerization-depolymerization processes. We found fast (5
Hz) pulsatory behavior with 1--2 nm amplitude on a cell with low motility
showing emphasized structure of stress fibres. Myosin driven contractions of
stress fibres are thought to induce this pulsation.Comment: 6 pages, 5 figures, 1 tabl
Predictive score for the development or progression of Graves' orbitopathy in patients with newly diagnosed Graves' hyperthyroidism
OBJECTIVE : To construct a predictive score for the development or progression of Gravesâ orbitopathy (GO) in Gravesâ hyperthyroidism (GH).
DESIGN : Prospective observational study in patients with newly diagnosed GH, treated with antithyroid drugs (ATD) for 18 months at ten participating centers from EUGOGO in 8 European countries.
METHODS : 348 patients were included with untreated GH but without obvious GO. Mixed effects logistic regression was used to determine the best predictors. A predictive score (called PREDIGO) was constructed.
RESULTS : GO occurred in 15% (mild in 13% and moderate to severe in 2%), predominantly at 6â12 months after start of ATD. Independent baseline determinants for the development of GO were clinical activity score (assigned 5 points if scoreâ>â0), TSH-binding inhibitory immunoglobulins (2 points if TBII 2â10âU/L, 5 points if TBIIâ>â10âU/L), duration of hyperthyroid symptoms (1 point if 1â4 months, 3 points if >4 months) and smoking (2 points if current smoker). Based on the odds ratio of each of these four determinants, a quantitative predictive score (called PREDIGO) was constructed ranging from 0 to 15 with higher scores denoting higher risk; positive and negative predictive values were 0.28 (95% CI 0.20â0.37) and 0.91 (95% CI 0.87â0.94) respectively.
CONCLUSIONS : In patients without GO at diagnosis, 15% will develop GO (13% mild, 2% moderate to severe) during subsequent treatment with ATD for 18 months. A predictive score called PREDIGO composed of four baseline determinants was better in predicting those patients who will not develop obvious GO than who will