GBCW Support for Shellfish Activities 2003

The Great Bay Coast Watch (GBCW) is a volunteer estuarine monitoring program established in 1989 that includes teachers, students, and local citizens with a diversity of backgrounds. Volunteers participate in a variety of training programs that enable them to monitor water quality parameters in Great Bay and coastal areas, sample for marine phytoplankton blooms and conduct shoreline surveys and habitat evaluations. Since 1997 the New Hampshire Estuaries Project (NHEP) has relied on the ability of GBCW to recruit and train volunteers to assist with the implementation of its plan to protect, restore and manage the states estuarine systems. This year GBCW again participated in plan implementation by assisting the NH Department of Environmental Services (NHDES) Shellfish Program. Volunteers completed a variety of work tasks, including mussel collection, sample collection and transport and general field assistance

2004 Support for Shellfish Program and Estuarine Education, Meeker, S & Reid, A

Working within the University of New Hampshire (UNH) Cooperative Extension/Sea Grant Program, the Great Bay Coast Watch (GBCW) is New Hampshire’s most wide ranging program for direct citizen involvement in monitoring estuarine and coastal systems. GBCW has a fifteen year history of educating citizens about the Great Bay Estuary, New Hampshire Seacoast, and Gulf of Maine watershed through active participation in monitoring and an accompanying education program. Based in Durham, NH, GBCW coordinates over 100 volunteers drawn from 19 New Hampshire and Southern Maine communities. In 1990, volunteers began monitoring eight sites on a monthly basis April through October. Today, this effort has grown to include 21 sites that are sampled monthly for water quality around the Great Bay estuary, and six coastal sites that are monitored weekly for harmful algae blooms. Volunteers include adults, students, and home schooled families

A SIMPLE ALTERNATIVE TO THE STANDARD STATISTICAL MODEL FOR THE ANALYSIS OF FIELD EXPERIMENTS WITH LATIN SQUARE DESIGNS

Latin Square (LS) designs have long been advocated for field crop experiments on the grounds that \u27. . . soil fertility and other variations in two directions are controlled.\u27 As counter-evidence, the published standard analyses of eight LS experiments showed that in only two did the sum of squares for both between-rows and between-columns account for appreciable background variability. Regarding the background concomitant variability as a continuous surface to which treatment effects are additive, it is suggested that a contributory shortcoming of the standard model is that it admits only a restricted class of surfaces because parameters for warp, or row x column interaction, components are excluded. It is shown that, at the loss of some orthogonality between background and treatment effects, the deficiency can be remedied by fitting a more general polynomial surface. The principle is exemplified using a backward selection mUltiple regression procedure to analyze LS data in Cochran and Cox (1957). The procedure gave a considerable reduction in the coefficient of variation, from 12.9 to 6.3%, and permitted more sensible inferences than those (null) from the standard analysis. A note on medieval cultivation practices and experimental design is appended

Great Bay Coast Watch: A Citizen Water Monitoring Program Volunteer Water Quality Monitoring Manual, 2004

The Great Bay Coast Watch is citizen volunteers, working within the UNH Cooperative Extension/NH Sea Grant Program, protecting the long-term health and natural resources of New Hampshire’s coastal waters and estuarine systems through monitoring and education projects. The purpose of this document is to present step-by-step instructions for conducting water quality testing in support of the Great Bay Coast Watch (GBCW)

Feline Immunodeficiency Virus Neuropathogenesis: From Cats to Calcium

Invasion of human immunodeficiency virus (HIV) into the central and peripheral nervous system produces a wide range of neurological symptoms, which continue to persist even with adequate therapeutic suppression of the systemic viremia. The development of therapies designed to prevent the neurological complications of HIV require a detailed understanding of the mechanisms of virus penetration into the nervous system, infection, and subsequent neuropathogenesis. These processes, however, are difficult to study in humans. The identification of animal lentiviruses similar to HIV has provided useful models of HIV infection that have greatly facilitated these efforts. This review summarizes contributions made from in vitro and in vivo studies on the infectious and pathological interactions of feline immunodeficiency virus (FIV) with the nervous system. In vivo studies on FIV have provided insights into the natural progression of CNS disease as well as the contribution of various risk factors. In vitro studies have contributed to our understanding of immune cell trafficking, CNS infection and neuropathogenesis. Together, these studies have made unique contributions to our understanding of (1) lentiviral interactions at the blood–cerebrospinal fluid (CSF) barrier within the choroid plexus, (2) early FIV invasion and pathogenesis in the brain, and (3) lentiviral effects on intracellular calcium deregulation and neuronal dysfunction. The ability to combine in vitro and in vivo studies on FIV offers enormous potential to explore neuropathogenic mechanisms and generate information necessary for the development of effective therapeutic interventions

Ethanol suppression of peripheral blood mononuclear cell trafficking across brain endothelial cells in immunodeficiency virus infection

Earlier studies suggested that the combination of alcohol use and immunodeficiency virus infection resulted in more severe neurologic disease than either condition individually. These deleterious interactions could be due to increased immune cell and virus trafficking or may result from interactions between ethanol and human immunodeficiency virus (HIV)-associated toxicity within the brain. To determine the extent to which increased trafficking played a role, we examined the effect of ethanol on the migration of different peripheral blood mononuclear cell (PBMCs) subsets across a brain endothelial cell monolayer. We utilized combinations of feline brain endothelial cells with astrocytes, and/or microglia with either acute exposure to 0.08 g/dL ethanol, a combination of ethanol and feline immunodeficiency virus (FIV), or FIV alone. Adherence of PBMCs to endothelium was increased in all combinations of cells with the addition of ethanol. Despite increased PBMC adhesion with ethanol treatment, transmigration of B cells, monocytes, CD4 T cells and CD8 T cells was not increased and was actually decreased in the presence of astrocytes. Expression of three common adhesion molecules, intercellular adhesion molecule-1 (ICAM1), ICAM2, and vascular cell adhesion molecule, was unchanged or slightly decreased by ethanol. This indicated that although adherence is increased by ethanol it is not due to an increased expression of adhesion molecules. RANTES, MIP1α, MIP1β, and MCP-1 mRNA expression was also studied in brain endothelial cells, astrocytes and microglia by reverse transcriptase-polymerase chain reaction. Ethanol treatment of astrocytes resulted in modest changes of message while FIV caused 7–92-fold increases. The combination of ethanol and FIV reversed the large increase in RANTES and MIP1α message in astrocytes but increased MIP1β and MCP to 20–38-fold over control cells. Thus, modest concentrations of alcohol do not directly influence immune cell trafficking at the endothelium but may exert more complex effects on chemokine expression from astrocytes when combined with FIV