Spatial organization of the mouse genome and its role in recurrent chromosomal translocations

The extent to which the three-dimensional organization of the genome contributes to chromosomal translocations is an important question in cancer genomics. We generated a high-resolution Hi-C spatial organization map of the G1-arrested mouse pro-B cell genome and used high-throughput genome-wide translocation sequencing to map translocations from target DNA double-strand breaks (DSBs) within it. RAG endonuclease-cleaved antigen-receptor loci are dominant translocation partners for target DSBs regardless of genomic position, reflecting high-frequency DSBs at these loci and their colocalization in a fraction of cells. To directly assess spatial proximity contributions, we normalized genomic DSBs via ionizing radiation. Under these conditions, translocations were highly enriched in cis along single chromosomes containing target DSBs and within other chromosomes and subchromosomal domains in a manner directly related to pre-existing spatial proximity. By combining two high-throughput genomic methods in a genetically tractable system, we provide a new lens for viewing cancer genomes

Evaluation of the Topography of Surface Sized Eucalyptus Based Papers

The surface properties of paper depend not only on the fibrous matrix but also on the final treatment of the paper surface. The present work compares paper samples with two different surface sizing treatments by using AFM and profilometry to assess topography and roughness parameters as well as to evaluate the spreading of the sizing formulation and to estimate sizing agent particle sizes. The results were confronted with dynamic light scattering measurements regarding particle size. This work shows that AFM is a valuable technique to visualize and quantify the effects of sizing on the paper surface. Due to the small amount of surface sizing applied, no significant differences between the surface sized samples and the reference sample (without surface treatment) could be detected in terms of the surface roughness parameters. Nonetheless, those of sample B1 are systematically smaller than those of sample A1