Acetosyringone induces the accumulation of a set of RNAs in the Arbuscular Mycorrhiza fungus Glomus intraradices

Plant root exudates contain a range of low molecular weight metabolites, which are believed to trigger the structural and physiological changes associated with the progression and establishment of the mycorrhizal symbiosis. Glomus intraradices spores were incubated with acetosyringone (AS), and an overall increase in the hyphal respiration was observed, indicating a physiological response triggered by this plant regulator. A G. intraradices cDNA library was then screened with a total cDNA probe obtained from the AS-treated spores and mycelium. The cDNAs from different functional categories were identified as induced in G. intraradices when exposed to AS, such as protein synthesis, membrane transport, signal transduction, general metabolism, without assigned function, and no identity. Interestingly, a cDNA coding for a fragment of a histidine kinase was also induced by AS, suggesting a two-component mediated response. We also demonstrated the differential accumulation of a cruciform DNA-binding protein mRNA, termed as GiBP1. Time-course experiments demonstrated its rapid accumulation within 2h of induction with AS. These results indicate the presence of a set of fungal genes that are induced by the presence of the inducer. These findings are discussed in terms of the possible molecular events that follow the exchange of signals between the mycorrhizal symbionts

Dataset of Arabidopsis plants that overexpress FT driven by a meristem-specific KNAT1 promoter

In this dataset we integrated figures comparing leaf number and rosette diameter in three Arabidopsis FT overexpressor lines (AtFTOE) driven by KNAT1 promoter, “A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis” [5], vs Wild Type (WT) Arabidopsis plats. Also, presented in the tables are some transcriptomic data obtained by RNA-seq Illumina HiSeq from rosette leaves of Arabidopsis plants of AtFTOE 2.1 line vs WT with accession numbers SRR2094583 and SRR2094587 for AtFTOE replicates 1–3 and AtWT for control replicates 1–2 respectively. Raw data of paired-end sequences are located in the public repository of the National Center for Biotechnology Information of the National Library of Medicine, National Institutes of Health, United States of America, Bethesda, MD, USA as Sequence Read Archive (SRA). Performed analyses of differential expression genes are visualized by Mapman and presented in figures. “Transcriptomic analysis of Arabidopsis overexpressing flowering locus T driven by a meristem-specific promoter that induces early flowering” [2], described the interpretation and discussion of the obtained data. Keywords: Differential expression, Bioinformatics, Flowerin

Macromolecular trafficking between a vesicular arbuscular endomycorrhizal fungus and roots of transgenic tobacco

The root system of transgenic tobacco plants expressing the enhanced green fluorescent protein (EGFP) under the control of the 35S cauliflower mosaic virus (CaMV) promoter were colonized with the endomycorrhizal fungus Glomus intraradices. Translocation of EGFP protein from the root to the fungus was registered by light and confocal microscopy. Immunolocalization also showed the presence of EGFP in the mycelium of Glomus intraradices. Carboxyfluorescein feeding experiments on wild-type mycorrhized plants evidenced the transport of fluorescein, a symplasmic tracer, from the plant to the fungus. Our results suggest that endomycorrhiza possess the capacity to exchange functional biological macromolecules as evidenced by the transport EGFP from the plant to the fungal symbiont