Phytochemical and in-vitro antimicrobial screening of Sanseviera liberica Gérôme and Labroy (Agavaceae) root extract

Sanseviera liberica is used in Nigerian folk medicine for the treatment of asthma, abdominal pain, diarrhea, wounds of the foot, gonorrhea, snake bites etc. Some of these diseases are caused by pathogenic bacteria. To validate the trado-medical applications of S. liberica, an investigation of the phytochemical and antimicrobial properties of the methanol and n – hexane extracts was conducted. Phytochemical screening revealed the presence of tannins, flavonoids, saponins, reducing sugars and   carbohydrates in both extracts. Alkaloids were present only in the methanol extract. Glycosides, terpenoids and steroids were absent in both extracts. The proximate analysis of S. liberica showed total ash value of 6.0 %, acid insoluble ash of 1.10 %, alcohol extractive value of 6.80 %, water  extractive value of 5.20 % and moisture content of 14.50 %.The broth microdilution technique was used to evaluate the antimicrobial activities ofboth the methanol and n – hexane extracts of S. liberica. Results showed that Psuedomonas. aureginosa, Streptococci pyrognase and Staphylococcus aureus were sensitive to the methanol extract while Candida albicans,  Klebsiella pneumonia, Salmonella typhii, Baccilus subtilis and Escherischia coli were not sensitive. All the eight microorganisms involved in the  antimicrobial assay were not sensitive to the n-hexane extract. © JASE

Prediction of emissions and profits from a biomass, tyre, and coal fired co-gasification CHP plant using artificial neural network: Nigerian and South African perspectives

Abstract : The local sourcing of feedstock for energy generation will reduce costs in the power plant, and promote energy sustainability. Most times, potential investors in this area show interest about understanding the profitability of the business because, the information boosts the confidence of the investors in the project, and gives them the opportunity of making a short and long term plans about the business. The emissions arising from the energy plant is an important aspect of the venture that requires proper attention, otherwise the costs of emission control may consume a greater part of the profit, hence rendering the business un-viable. Nigeria and South Africa (SA) have abundant biomass (e.g. corn cob, sugarcane bagasse, & pine saw dust) coal and tyre that can be used as fuel in an energy plant. A 10 MW CHP plant was fired with coal and biomass, and tyre obtained from Nigeria and South Africa (SA) respectively, at ratios of 1:1, 3:2, and 4:1 to study the emissions and profits in the plant. An empirical model was employed to estimate the annual amount of feedstock and feed rate required for the plant, after which, an artificial neural network (ANN); LevenbergMarquardt algorithm was used to predict the emissions and profits in the plant for 20-year- investment period with feedstock costing (WFC) and without feedstock costing (WOFC). The profit obtained from the South African feedstock, WFC and WOFC; produced about 45.18 % and 36.83 % ($3,900,000.07 and$3,179,184.49) higher profits than the Nigerian feedstock, but the CO, NOX, & SO2 emissions from Nigerian feedstock were lower than that of SA. The findings from this study could be used as a platform for decision making by potential investors and stake-holders, and further research and development in the area

Prostate cancer disparities in Black men of African descent: a comparative literature review of prostate cancer burden among Black men in the United States, Caribbean, United Kingdom, and West Africa

<p>Abstract</p> <p>Background</p> <p>African American men have the highest prostate cancer morbidity and mortality rates than any other racial or ethnic group in the US. Although the overall incidence of and mortality from prostate cancer has been declining in White men since 1991, the decline in African American men lags behind White men. Of particular concern is the growing literature on the disproportionate burden of prostate cancer among other Black men of West African ancestry in the Caribbean Islands, United Kingdom and West Africa. This higher incidence of prostate cancer observed in populations of African descent may be attributed to the fact that these populations share ancestral genetic factors. To better understand the burden of prostate cancer among men of West African Ancestry, we conducted a review of the literature on prostate cancer incidence, prevalence, and mortality in the countries connected by the Transatlantic Slave Trade.</p> <p>Results</p> <p>Several published studies indicate high prostate cancer burden in Nigeria and Ghana. There was no published literature for the countries Benin, Gambia and Senegal that met our review criteria. Prostate cancer morbidity and/or mortality data from the Caribbean Islands and the United Kingdom also provided comparable or worse prostate cancer burden to that of US Blacks.</p> <p>Conclusion</p> <p>The growing literature on the disproportionate burden of prostate cancer among other Black men of West African ancestry follows the path of the Transatlantic Slave Trade. To better understand and address the global prostate cancer disparities seen in Black men of West African ancestry, future studies should explore the genetic and environmental risk factors for prostate cancer among this group.</p

Potential of Cashew Leaf Extract for Mild Steel Corrosion Mitigation in Hydrochloric Acid Environment

This study investigated cashew leaf\u27s potential to reduce mild steel (MS) corrosion in an HCl environment. With the use of a gas chromatography-mas spectrophotometer, the molecular components of the extract were determined. The adsorption mechanism and characteristics of the corrosion inhibition process were determined using the gravimetric approach. Furthermore, artificial neural networks (ANN) and response surface methodology (RSM) were used to forecast the cashew leaf extract\u27s inhibitory efficacy. According to the results, the main biomolecular components of the cashew leaf extract were 9-tetradecenal, oleic acid, 2-hydroxy cyclopenta decanone, 4H-pyran-4-one, 3,5-dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one, 2-methyl-3-ketotetrahydrofuran, oxirane, 8-methyl-9-tetradecenoic acid, 2,3-butylene oxide, 2,3-dimethyloxirane and 3(2H)-furanone. The biomolecules spontaneously adhered to the MS surface, a physical adsorption event. The results of the optimization/prediction analyses demonstrated that when it came to forecasting the extract\u27s efficacy, ANN outperformed RSM. The study’s findings can serve as the basis for using cashew leaf as an additive to prevent mild steel from corroding in a corrosive environment

Blood Derived Amyloid Biomarkers for Alzheimer's Disease Prevention.

BACKGROUND: Reliable, widely accessible and affordable biomarkers for predicting Alzheimer's disease (AD) brain pathology status are a necessity to aid development of prevention strategies in cognitively healthy at-risk older adults, at the right timepoint. Measurements of the key neuropathological hallmark beta-amyloid (Aβ) by PET neuroimaging or cerebrospinal fluid measures reflect its accumulation in the brain, yet recent methodological advancements now enable blood-based measures reflecting cerebral amyloid burden. OBJECTIVES: The current study validated the capacity of plasma Aβ42/Aβ40 measured using six different assays to predict amyloid positivity in a subgroup of cognitively unimpaired (CU) participants in the ADNI study and assessed its ability to discriminate CU from AD cases. We also explored economic viability of using two different plasma amyloid assays for pre-screening in AD prevention trials and as routine clinical diagnostic tool, versus amyloid PET alone. DESIGN: A cross-sectional analysis of plasma and brain amyloid data, including comparative cost analysis of the plasma biomarkers in relation to brain amyloid PET. SETTING: Alzheimer's Disease Neuroimaging Initiative (ADNI). PARTICIPANTS: ADNI participants consisting of 115 CU, mild cognitive impairment and AD cases who had plasma Aβ42/Aβ40 measured with six platforms. MEASUREMENTS: Plasma Aβ42/Aβ40 was measured via six different platforms: three immunoassays (Roche, Quanterix and ADx Neurosciences) and three mass spectrometry (MS) based assays (WashU, Shimadzu and Gothenburg). Aβ-PET imaging was conducted within three months of plasma sampling using [18F]florbetapir. RESULTS: There was a weak to moderate correlation of plasma Aβ42/Aβ40 ratio between platforms. The MS-based WashU test had the highest capacity to discriminate between CU and AD (area under the curve, AUC = 0.734, 95% CI: 0.613-0.854; P = 0.008). Within the CU group, the WashU plasma amyloid test had the best discriminative capacity to distinguish Aβ+ from Aβ- (AUC = 0.753, 95% CI: 0.601-0.905; P = 0.003) closely followed by the immunoassay from Roche (AUC = 0.737, 95% CI: 0.597-0.877; P = 0.006). The exploratory economic analyses showed that the use of Roche or WashU plasma amyloid assay as a pre-screening tool prior to Aβ-PET scans for clinical trial recruitment significantly reduced total screening cost (saving up to $5882 per recruited patient) expected in an AD prevention trial. CONCLUSIONS: With few available treatment strategies, dementia prevention is a global priority. CU individuals at risk for AD are the target population for dementia prevention but have been poorly studied. Our findings confirming diagnostic value of ultrasensitive immunoassays and high-performance immunoprecipitation coupled with MS for measurement of plasma Aβ42/Aβ40 to detect PET amyloid positivity in CU participants allude to potential clinical utility of this biomarker. Plasma Aβ42/Aβ40 could be optimal for pre-selecting at-risk candidates for more invasive and expensive investigations across AD prevention clinical trials and clinical care for a rapidly ageing population

Students' awareness, willingness and utilisation of facebook for research data collection: Multigroup analysis with age and gender as control variables

Previous research has extensively analysed teachers' and students' Facebook use for instructional engagement, writing, research dissemination and e-learning. However, Facebook as a data collection mechanism for research has scarcely been the subject of previous studies. The current study addressed these gaps by analysing students' awareness, willingness, and utilisation of Facebook for research data collection [RDC]. This study aimed to predict students’ Facebook use for research data collection based on their awareness and willingness and to determine age and gender differences in such predictions. A sample of 11,562 students of tertiary institutions participated in an online survey. The researchers designed and validated the online survey. Exploratory Factor Analysis was used for dimensionality. Average Variance Extracted [AVE] was used for convergent validity, whilst the Fornel-Larcker criterion and Heterotrait-Monotrait ratio were used for discriminant validity. Composite reliability indices of .97, .94 and .90 proved the instrument’s three sub-scales usable. One sample t-test and multigroup analysis were conducted using SPSS 27 and Smart PLS 3. The study found high awareness but low usage of Facebook for RDC among Nigerian university students. Younger and male students showed higher willingness and actual use of Facebook for RDC. Awareness directly impacted by awareness, and acted as Facebook usage for RDC, with stronger impacts for males and students aged 19 to 28. Willingness was positively impacted and acted as a negative mediator between awareness and usage for some age groups and genders. These findings provide important insights into using Facebook for RDC among students in Nigerian universities and highlight the need to consider the impact of demographic factors such as age and gender when promoting social media platforms for academic purposes

Alzheimer's disease pathology explains association between dementia with Lewy bodies and APOE-ε4/TOMM40 long poly-T repeat allele variants.

Introduction: The role of TOMM40-APOE 19q13.3 region variants is well documented in Alzheimer's disease (AD) but remains contentious in dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD). Methods: We dissected genetic profiles within the TOMM40-APOE region in 451 individuals from four European brain banks, including DLB and PDD cases with/without neuropathological evidence of AD-related pathology and healthy controls. Results: TOMM40-L/APOE-ε4 alleles were associated with DLB (OR TOMM40 -L = 3.61; P value = 3.23 × 10-9; OR APOE -ε4 = 3.75; P value = 4.90 × 10-10) and earlier age at onset of DLB (HR TOMM40 -L = 1.33, P value = .031; HR APOE -ε4 = 1.46, P value = .004), but not with PDD. The TOMM40-L/APOE-ε4 effect was most pronounced in DLB individuals with concomitant AD pathology (OR TOMM40 -L = 4.40, P value = 1.15 × 10-6; OR APOE -ε4 = 5.65, P value = 2.97 × 10-8) but was not significant in DLB without AD. Meta-analyses combining all APOE-ε4 data in DLB confirmed our findings (ORDLB = 2.93, P value = 3.78 × 10-99; ORDLB+AD = 5.36, P value = 1.56 × 10-47). Discussion: APOE-ε4/TOMM40-L alleles increase susceptibility and risk of earlier DLB onset, an effect explained by concomitant AD-related pathology. These findings have important implications in future drug discovery and development efforts in DLB

DISTRIBUTION AND ABUNDANCE OF ECTO AND ENDO HELMINTH PARASITES OF Clarias gariepinus FROM NATURAL AND ARTIFICIAL HABITATS IN ANAMBRA STATE, SOUTHEASTERN NIGERIA.

Helminth parasites have been recognized as a major threat to fish health and cause reduced economic gains worldwide. This study aimed to determine the abundance and distribution of ecto and endo helminth parasites of Clarias gariepinus from natural and artificial habitats in Anambra state, Nigeria. A total of 40 live adult Clarias gariepinus (15.67–440.08 g body weight) were randomly purchased and examined for ecto and endo helminth parasites. The fish consists of 15 each from natural and 10 from artificial habitats. Standard parasitological techniques were used for the isolation and identification of the ecto and endo helminth parasites. Data obtained was analysed with ꭓ2 at 5 % significance level. Nineteen (48%) of Clarias gariepinus sampled were infected with 59 different species of helminth parasites. Endoparasites identified were: Procamallanus sp. (25%), Camallanus sp. (37.5%), Polyonchobothrium sp. (7.5%), and Protocephalus sp. (2.5%) with one ectoparasite Gyrodactylus sp. (2.5%) (P < 0.05). Natural and artificial habitats have a prevalence of 48% and 0% (P < 0.05) respectively. Catfish obtained from Otuocha section of Omambala River habitat with body weight 100-199g, has the highest prevalence (75%), while highest parasite distribution in organs was found in the intestines (40.0%). The higher infection rate observed among natural habitat fish indicates river contamination. Good hygienic practice is advocated among the community members

Prevalence of intestinal parasitic infections among HIV patients in Benin City, Nigeria

This study was carried out to determine the presence of intestinal parasites and their correlation with CD4+ T-cell counts and demographics among human immunodeficiency virus (HIV)-positive patients in Benin City, Nigeria. Stool specimens from 2,000 HIV-positive patients and 500 controls (HIV-negative individuals) were examined for ova, cysts, or parasites, using standard procedures. In addition, patient's blood samples were analyzed for CD4 counts by flow cytometry. An overall prevalence rate of 15.3% was observed among HIV-positive patients while 6.2% was noted among non-HIV subjects. HIV status was a significant (P<0.0001) risk factor for acquiring intestinal parasitic infections. Male gender, CD4 count <200cell/µl, and diarrhea were significantly associated with an increased prevalence of intestinal parasitic infections among HIV-positive patients. The level of education, occupation, and source of water among HIV patients significantly (P<0.0001) affected the prevalence of intestinal parasitic infections. Ascaris lumbricoides was the most predominant parasite in both HIV-positive patients and controls. A CD4 count <200 cells/µl was significantly associated with only Isospora belli and Cryptosporidium infections. The presence of pathogenic intestinal parasites such as A. lumbricoides, hookworm, Giardia intestinalis, Entamoeba histolytica, Trichuris trichiura, and Taenia species among HIV-infected persons should not be neglected. Cryptosporidium species and I. belli were the opportunistic parasites observed in this study. Routine screening for intestinal parasites in HIV-positive patients is advocated

Clinical reporting following the quantification of cerebrospinal fluid biomarkers in Alzheimer's disease: An international overview

Introduction: The current practice of quantifying cerebrospinal fluid (CSF) biomarkers as an aid in the diagnosis of Alzheimer's disease (AD) varies from center to center. For a same biochemical profile, interpretation and reporting of results may differ, which can lead to misunderstandings and raises questions about the commutability of tests. Methods: We obtained a description of (pre-)analytical protocols and sample reports from 40 centers worldwide. A consensus approach allowed us to propose harmonized comments corresponding to the different CSF biomarker profiles observed in patients. Results: The (pre-)analytical procedures were similar between centers. There was considerable heterogeneity in cutoff definitions and report comments. We therefore identified and selected by consensus the most accurate and informative comments regarding the interpretation of CSF biomarkers in the context of AD diagnosis. Discussion: This is the first time that harmonized reports are proposed across worldwide specialized laboratories involved in the biochemical diagnosis of AD