Studies on influence of modified atmospheric storage conditions on biochemical parameters in pigeonpea seeds

An experiment was conducted to study the influence of modified atmospheric storage conditions on biochemical parameters of pigeonpea seeds. The experiment was conducted at the Department of Seed Science and Technology, College of Agriculture, Raichur, Karnataka. The seeds are exposed to various gaseous combinationsand stored in 700 gauge polyethylene bag for ten months during July-2012 to April- 2013. The results revealed that, the seeds exposed to gaseous combination of 40% N2+00% O2+ 60% CO2 showed less reduction in dehydrogenase enzyme activity and protein content (0.276 OD (optical density) value, 19.33 % respectively) as compared to the control (0.211 OD value and 18.13 % respectively) after ten months of storage. In addition less seed leachate (2.029 dSm-1) was recorded in gaseous combination of 40% N2+00% O2+ 60% CO2 as compared to control (2.207dSm-1). It indicates the potential use of modified atmospheric storage technology for maintenance of seed viability and vigour during storage in pulses

Enhancement of solubility and oral bioavailability of poorly soluble drug Valsartan by novel solid self emulsifying drug delivery system

The main objective of present work was to prepare a solid SEDDS for enhancement of oral bioavailability of Valsartan, poorly water soluble drug. The solubility of the drug was determined in various vehicles. A pseudo ternary phase diagram was constructed to identify the self-micro emulsification region. Further, the resultant formulations were investigated for clarity, phase separation, globule size, effect of pH and dilutions and freeze-thaw stability. The optimized SMEDDS (F4) formulation of Valsartan contained Capmul MCM (Oil), Kolliphor HS 15 (Surfactant) and PEG 400 (Co-surfactant). This optimized formulation was converted in to solid SEDDS by adding required quantity of Neusilin US2 as adsorbing agent used for in vitro dissolution and bioavailability assessment. The oral bioavailability of Valsartan from solid SEDDS was 1.6-fold higher compared to that of Valsartan suspension in rats, suggesting a significant increase (p < 0.05) in oral bioavailability of Valsartan from solid SEDDS

RECENT PROGRESS OF DENDRIMERS IN DRUG DELIVERY FOR CANCER THERAPY

With the recent advances of nanotechnology, dendrimers are emerging as a highly attractive class of drug delivery vectors for cancer therapy. Dendrimers are multifunctional smart Nanocarriers to deliver one or more therapeutic agent safely and selectively to cancer cells. The high level of control over the synthesis of dendritic architecture makes dendrimers a nearly perfect (spherical) nanocarrier for site-specific drug delivery. The presence of functional groups in the dendrimers exterior also permits the addition of other moieties that can actively target certain diseases which are now widely used as tumor targeting strategies. Drug encapsulation, solubilization and passive targeting also equally contribute to the therapeutic use of dendrimers. Dendrimers are ideal carrier vehicles on cytotoxicity, blood plasma retention time, biodistribution and tumor uptake. In this review we highlight the advantages of dendrimers over conventional chemotherapy, toxicity and its management, following anti-cancer drugs delivered by using dendrimers and recent advances in drug delivery by various types of dendrimers as well as its diagnostic applications

CURRENT DISTRIBUTION AND REGULATORY SYSTEM FOR CELL ENERGY

Within this paper, a coordinated and multivariable energy management technique is suggested which uses a wind generator along with a photovoltaic variety of a standalone Electricity microgrid as controllable generators by modifying the pitch position and also the switching duty cycles. Because of substantial generation and demand fluctuations in standalone eco-friendly micro grids, energy management strategies have become required for the ability discussing and current regulation purposes. The classical energy management strategies employ the utmost power point tracking (MPPT) algorithms and depend on batteries in situation of possible excess or deficit of one's. However, to be able to realize constant current-constant current (IU) charging regime while increasing the life time of batteries, energy management strategies require being more flexible using the power curtailment feature. The variable load demands will also be shared precisely between generators compared for their ratings. Furthermore, the Electricity bus current is controlled inside a predefined range, like a design parameter. The suggested technique is developed being an online nonlinear model predictive control (NMPC) formula. Signing up to an example standalone electricity microgrid, the developed controller realizes the IU regime for charging battery bank

Hysterectomy in uterine fibroids: an institutional survey pre and post COVID lockdown

Background: The COVID-19 pandemic had a tremendous effect on surgical practice and surgical outcomes, especially in Gynaecology which had led to an increase in operative interferences like hysterectomies, which is one of the most frequently performed surgical procedures for uterine leiomyomas. Aim of this study was to determine the impact of covid lockdown on hysterectomies performed on AUB–L in perimenopausal and post-menopausal women based on clinical, radiological, and operative aspects. Methods: A retrospective study on perimenopausal and post-menopausal women who underwent hysterectomy for AUB-L was compared based on their clinical, radiological, and operative findings in the pre COVID period (April 2019-March 2020) and post COVID lockdown period of one year (July 2021-June 2022), at Department of Obstetrics and Gynaecology, SVS Hospital, Telangana. Results: The post-covid group has a significant increase in the severity of clinical symptoms such as menorrhagia and dysmenorrhea (VMSS-GRADE 2). Radiological findings (US AND MRI)-The size (>5cms) and number (>2) of fibroids increased significantly in post COVID group. The operative duration (1-3hrs), intraoperative blood loss (150-300ml), need for blood transfusion (>24%), postoperative pain (VAS pain score 5), and mean hospital stay (72-120hrs) were significantly increased in the post-COVID group as compared to pre COVID group attributable to delay in seeking medical advice due to COVID-19 lockdown. Conclusions: This study clearly states that the impact of the COVID-19 lockdown was significant in worsening clinical features, increasing the size and number of fibroids which in turn increased the number of hysterectomies, operative time and complications

Enhancement of solubility and oral bioavailability of poorly soluble drug Valsartan by novel solid self emulsifying drug delivery system

The main objective of present work was to prepare a solid SEDDS for enhancement of oral bioavailability of Valsartan, poorly water soluble drug. The solubility of the drug was determined in various vehicles. A pseudo ternary phase diagram was constructed to identify the self-micro emulsification region. Further, the resultant formulations were investigated for clarity, phase separation, globule size, effect of pH and dilutions and freeze-thaw stability. The optimized SMEDDS (F4) formulation of Valsartan contained Capmul MCM (Oil), Kolliphor HS 15 (Surfactant) and PEG 400 (Co-surfactant). This optimized formulation was converted in to solid SEDDS by adding required quantity of Neusilin US2 as adsorbing agent used for in vitro dissolution and bioavailability assessment. The oral bioavailability of Valsartan from solid SEDDS was 1.6-fold higher compared to that of Valsartan suspension in rats, suggesting a significant increase (p < 0.05) in oral bioavailability of Valsartan from solid SEDDS

A review of antibiotic synergy in carbapenemase-producing bacteria

The problem of antibiotic resistance has garnered too much attention over the last few decades for posing a global hazard to the clinical handling and the inhibition of several deadly infections caused by bacteria. It burdens the world not only clinically but also economically... Antibiotic agents known as carbapenems are a very effective and  typically designated for the treatment of multidrug-resistant (MDR) bacterial infections. To identify a suitable antibiotic combination to be used in vivo, one must be able to determine the synergism between the antibiotics in vitro. Several methods, such as the checkerboard method, multiple-combination bactericidal test, time-kill and E-test, have been used for this purpose. However, the lack of proper standardization procedures, types of bacterial agents, bacterial load, stage of infection and other factors make it very difficult to reproduce or correlate the results with other methods.Carbapenem-destroying lactases, which have recently emerged as mechanisms of resistance, are increasing in number and decreasing the treatment alternatives available. These infections are treated with colistin and tigecycline, but monotherapy may result in clinical breakdown because of a variety of factors. To control these infections, clinicians often choose combinations of drugs over monotherapy. There is an extreme lack of information on synergistic antibiotic combinations accounting for the diverse mechanisms of GNB resistance commonly encountered. The incidence of carbapenem-resistant GNB in Indian articles is also unknown. Therefore, we anticipate that this study may provide methodology for the selection of an appropriate antibiotic combination

Facts and Treatment of Bullous Pemphigoid: A Review

Bullous pemphigoid (a type of pemphigoid) is an autoimmune pruritic skin disease that typically occurs in people aged over 60, that may involve the formation of blisters (bullae) in the space between the epidermal and dermal skin layers. It is classified as a type II hypersensitivity reaction, which involves formation of anti-hemidesmosome antibodies, causing a loss of keratinocytes to basement membrane adhesion. Pemphigus and bullous pemphigoid are autoantibody-mediated blistering skin diseases. In pemphigus, keratinocytes in epidermis and mucous membranes lose cell-cell adhesion, and in pemphigoid, the basal keratinocytes lose adhesion to the basement membrane. Pemphigus lesions are mediated directly by the autoantibodies, whereas the autoantibodies in pemphigoid fix complement and mediate inflammation. In both diseases, the autoantigens have been cloned and characterized; pemphigus antigens are desmogleins (cell adhesion molecules in desmosomes), and pemphigoid antigens are found in hemidesmosomes (which mediate adhesion to the basement membrane). This knowledge has enabled diagnostic testing for these diseases by enzyme-linked immunosorbent assays and dissection of various pathophysiological mechanisms, including direct inhibition of cell adhesion, antibody-induced internalization of antigen, and cell signaling. Understanding these mechanisms of disease has led to rational targeted therapeutic strategies

Probiotics-A Legacy of Good Health

Probiotics-means live microorganisms, which when administered in adequate amounts, confer a health benefit on the host. Probiotics can be bacteria, moulds, yeast. But most Probiotics are bacteria mainly lactic acid bacteria. The Probiotics have a three step action mechanism i. Stimulates and modulates immune response ii. Normalize intestinal micro flora iii. And also have the metabolic effects like. Probiotics have been analyzed for treatment and prevention of various diseases and disorders of human body and the results obtained are very encouraging. Probiotics have turned out to be very promising in ensuring oral health and general well being

“Beads-on-a-string” star formation tied to one of the most powerful active galactic nucleus outbursts observed in a cool-core galaxy cluster

With two central galaxies engaged in a major merger and a remarkable chain of 19 young stellar superclusters wound around them in projection, the galaxy cluster SDSS J1531+3414 (z = 0.335) offers an excellent laboratory to study the interplay between mergers, active galactic nucleus (AGN) feedback, and star formation. New Chandra X-ray imaging reveals rapidly cooling hot (T ∼ 106 K) intracluster gas, with two “wings” forming a concave density discontinuity near the edge of the cool core. LOFAR 144 MHz observations uncover diffuse radio emission strikingly aligned with the “wings,” suggesting that the “wings” are actually the opening to a giant X-ray supercavity. The steep radio emission is likely an ancient relic of one of the most energetic AGN outbursts observed, with 4pV > 1061 erg. To the north of the supercavity, GMOS detects warm (T ∼ 104 K) ionized gas that enshrouds the stellar superclusters but is redshifted up to +800 km s−1 with respect to the southern central galaxy. The Atacama Large Millimeter/submillimeter Array detects a similarly redshifted ∼1010 M ⊙ reservoir of cold (T ∼ 102 K) molecular gas, but it is offset from the young stars by ∼1–3 kpc. We propose that the multiphase gas originated from low-entropy gas entrained by the X-ray supercavity, attribute the offset between the young stars and the molecular gas to turbulent intracluster gas motions, and suggest that tidal interactions stimulated the “beads-on-a-string” star formation morphology