Anthelmintic Activity of Trikatu Churna and its Ingredients

The alcoholic extract of Trikatu churna and its ingredients were evaluated for anthelmintic activity. The dried fruits of Piper nigrum L. (Piperaceae), Piper longum L. (Piperaceae) and rhizome of Zingiber officinale Roscoe. (Zingiberaceae) were powdered and mixed together in equiproportions to get a polyherbal formulation, Trikatu churna. All these three ingredients are spicy, commonly used in our daily diet, also well known for their tremendous therapeutic potential, since from the Vedic period. The alcoholic extract of Trikatu churna and its ingredients were screened for preliminary phytochemical studies and also tested for anthelmintic activity against Pheritima posthuma and recorded the time taken for induction of paralysis and death. Piperazine citrate (10 mg/ml) was included as standard reference and distilled water as control. The results demonstrated that, the extracts of Trikatu churna and its plant ingredients showed the presence of alkaloids, flavonoids, tannins, lignins and steroids, these test samples were also exhibited potent anthelmintic activity, but the highest activity was noticed in Trikatu churna, this might be due to the multifunctional effect of all the three plant ingredients of Trikatu churna. Based on the above results, it is confirmed that, combination of Piper nigrum, Piper longum and Zingiber officinale in Trikatu churna offered promising anthelmintic effect than using the ingredients alone

Dynamical quantum Cherenkov transition of fast impurities in quantum liquids

The challenge of understanding the dynamics of a mobile impurity in an interacting quantum many-body medium comes from the necessity of including entanglement between the impurity and excited states of the environment in a wide range of energy scales. In this paper, we investigate the motion of a finite mass impurity injected into a three-dimensional quantum Bose fluid as it starts shedding Bogoliubov excitations. We uncover a transition in the dynamics as the impurity's velocity crosses a critical value which depends on the strength of the interaction between the impurity and bosons as well as the impurity's recoil energy. We find that in injection experiments, the two regimes differ not only in the character of the impurity velocity abatement, but also exhibit qualitative differences in the Loschmidt echo, density ripples excited in the BEC, and momentum distribution of scattered bosonic particles. The transition is a manifestation of a dynamical quantum Cherenkov effect, and should be experimentally observable with ultracold atoms using Ramsey interferometry, RF spectroscopy, absorption imaging, and time-of-flight imaging.Comment: 5 pages, 3 figures + 4 pages, 3 figure

Prospective study of the functional outcome of uncemented bipolar hemiarhroplasty in displaced fracture neck of femur in elderly

Background: Hip fractures in older patients are associated with impaired mobility, excess morbidity, mortality and loss of independence. To avoid the drawbacks of internal fixation and for early mobilization, hemiarthroplasty is performed in elderly. The bipolar prosthesis was developed to overcome the shortcomings of the unipolar fixed-head prosthesis. An additional site of motion would be expected to decrease the amount of loosening, dislocation, and head migration. The aim of the study is to assess the functional outcome of the patients with fracture neck of femur treated with bipolar hemiarthroplasty.Methods: In this study, 20 patients above the age of 60 years with acute displaced fracture neck of femur underwent hemireplacement arthroplasty with bipolar prosthesis in Department of Orthopaedics, KIMS, Bangalore. The patients were followed up on 6th, 12th, 18th, 24th week post operatively. Functional outcome was assessed with Harris hip score.Results: In our study, mean average age was 72.3 years with predominantly female patients with 75% of the patients with left sided fracture. 95% of the patients had slight or no pain, 85% of patients had slight or no limp. 50% of the patients could walk without support, 65% of the patients could walk a distance of 6 blocks or more. Average Harris hip score was 82%.Conclusions: These results suggest functional outcome following bipolar hemiarthroplasty is good. Early mobilisation, pain free hip and with good level of activity is obtained following bipolar hemiarthroplasty

Trends in clinical and oncological outcomes of robot-assisted radical prostatectomy before and after the 2012 US Preventive Services Task Force recommendation against PSA screening: a decade of experience

Objective: To assess the influence of the 2012 US Preventive Services Task Force (USPSTF) recommendation against prostate-specific antigen (PSA)-based screening on oncological and functional outcomes following robot-assisted laparoscopic prostatectomy (RALP). Materials and Methods: We retrospectively analysed patients who underwent RALP between 2008 and 2018 with a minimum of 12-month follow-up from a prospectively collected institutional review board-approved database. The impact of the USPSTF recommendation against PSA screening on our surgical outcomes was assessed using a logistic regression model using two groups comprising patients treated before/after the USPSTF statement and indicating time trends for each successive year. Results: The mean preoperative PSA increased from 6.0 to 7.4 ng/mL after the USPSTF recommendation. We detected statistically significant time-trend changes after 2012, including an increase in the positive slope of Gleason ≥3 + 4 or ≥pT3 disease. We detected a fall in bilateral full nerve-sparing and an increase in partial nerve-sparing. The total positive surgical margin (PSM) rate increased after the USPSTF recommendation; however, PSM rates pertinent to each pathological stage did not change significantly after 2012. There was a significant negative trend change in the postoperative 12-month continence and potency rates, indicating a breakpoint in functional outcomes after 2012. We detected a 1.7-fold increase in 12-month biochemical recurrence (BCR) rates. The 12-month BCR, potency and continence rates were maintained in young (<55 years) patients with a Sexual Health Inventory for Men score >22 and low-volume disease. Conclusion: Since the USPSTF’s recommendation in 2012, we have seen a significant increase in the incidence of high-risk disease that has forced us to modify our approach to the procedure and the grade of nerve-sparing used, leading to a wider resection, in order to reduce PSMs. This has led to a decrease in postoperative functional recovery. Patients with favourable characteristics had good outcomes before and after the USPSTF’s recommendation, implying that the quality of surgery did not change over time

Gapless Assembly of Maize Chromosomes Using Long-Read Technologies

Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics. We use two independent assemblies and an optical map-based merging pipeline to produce a maize genome (B73-Ab10) composed of 63 contigs and a contig N50 of 162 Mb. This genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), and 53 Mb of the Ab10 meiotic drive haplotype. The data also reveal the internal structure of seven centromeres and five heterochromatic knobs, showing that the major tandem repeat arrays (CentC, knob180, and TR-1) are discontinuous and frequently interspersed with retroelements