Highly effective liquid and solid phase extraction methods to concentrate radioiodine isotopes for radioiodination chemistry

Radioactive iodine isotopes play a pivotal role in radiopharmaceuticals. Large-scale production of multi-patient dose of radioiodinated nuclear medicines requires high concentration of radioiodine. We demonstrate that tetrabutylammonium chloride and methyltrioctylamonium chloride are effective phase transfer reagents to concentrate iodide-124, iodide-125 and iodide-131 from the corresponding commercial water solutions. The resulting concentrated radioiodide, in the presence of either phase transfer reagent, does not hamper the chemical reactivity of aqueous radioiodide in the copper (II)-mediated one-pot three-component click chemistry to produce radioiodinated iodotriazoles

Urban green space and urban biodiversity: Kuala Lumpur, Malaysia

Urban green space plays an important role in enhancing the quality of environment especially for urban biodiversity. Declining biodiversity around the world has received much attention among academics, professionals and citizens. The United Nations has declared year 2010 as the ‘International Year of Biodiversity’. Urban biodiversity movement is important to ensure healthy city environments. Despite this ongoing movement, urban dwellers have little knowledge about conservation of biodiversity particularly wildlife. Moreover, biodiversity has received little attention from built environment practices (Brown & Grant, 2005; Niemelä, 1999). In general, urban wildlife habitat is protected as reserved forest. Many species of wildlife are generally found in such reserved forests. In comparison, the scale of urban parks in Kuala Lumpur is massive, much larger than protected forests. This paper presents the situation of urban wildlife species and the quality of habitats in Kuala Lumpur. It is found that many factors contribute to the healthy conservation of urban wildlife such as quality and variety of habitats, ornamental versus native vegetation and ecological design. The findings highlight that common urban birds are dominant in urban green spaces regardless of the size of the green spaces. However, only larger urban green spaces help protect species. Finally, the study concludes that urban wildlife in Kuala Lumpur is rapidly declining and that there is a greater need for the community and stakeholders to promote programs and activities to preserve and enhance urban wildlife. Development of comprehensive acts, policies and guidelines are vital for urban wildlife protection. Keywords: urban wildlife, ecological design, wildlife management, urban green space, conservatio

Renoprotective effect of tectorigenin glycosides isolated from Iris spuria L. (Zeal) against hyperoxaluria and hyperglycemia in NRK-49Fcells

Oxidative stress has been identified as an underlying factor in the development of insulin resistance, β-cell dysfunction, impaired glucose tolerance and type 2 diabetes mellitus and it also play major role in kidney stone formation. The present study is aimed to elucidate the in vitro nephroprotective activity of two isoflavonoid glycosides, tectorigenin 7-O-β-D-glucosyl-(1→6)-β-D-glucoside (1) and tectorigenin 7-O-β-D-glucosyl-4'-O-β-D-glucoside (2) isolated from the n-BuOH fraction of Iris spuria L. (Zeal) rhizome MeOH extract against oxalate and high glucose-induced oxidative stress in NRK-49F cells. The results revealed that compounds 1 and 2 significantly increased the antioxidant enzyme activities and decreased MDA levels in both oxalate and high glucose stress. Treatment with these phytochemicals effectively down-regulated expression of crystal modulator genes and pro-fibrotic genes in oxalate and high glucose-mediated stress respectively. This study indicates cytoprotective, antioxidant, anti-urolithic and anti-diabetic effects of compounds 1 and 2 against oxalate and high glucose stress

Documenting Effective e-Ticketing Implementation

693JJ319D000030/693JJ320F000474The practice of printing, collecting, maintaining, and retaining paper tickets is cumbersome, less safe, outdated, more expensive, less efficient, and less sustainable than paperless ticketing. The adoption of electronic construction (e-Construction) technologies in construction administration and delivery offers ways to overcome these inefficiencies. By leveraging the groundwork laid by e-Construction, electronic ticketing (e-Ticketing) has attracted wider interest by transportation agencies as an electronic solution for material delivery. e-Ticketing is a digital innovation that automates the recording and transfer of information in realtime for materials as they are moved from the production plant to the site. Since the first e-Ticketing pilot in 2015, the implementation landscape has been rapidly emerging. The 2020 construction season made the move to e-Ticketing even more relevant by increasing the need for touchless operations. State transportation agencies (STAs) have a diversity of practices regarding material types for which e-Ticketing is implemented, such as vendor and in-house solutions, and implementation approaches. In this context, this study documents the state of the practice and the state of the knowledge of e-Ticketing. The findings of the nationwide survey, conducted by the American Association of State Highway and Transportation Officials, on the practice of e-Ticketing, benefits and costs, implementation factors and challenges, and future plans of STAs are documented. Through the documentation of case studies, this study discusses the piloting experiences of nine STAs. In addition, this study presents a business case by documenting the strategic value of e-Ticketing with an illustration of benefits quantification. The study also presents additional information for implementation planning based on the analysis of the case study interviews, assessment of practice landscape, and review of emerging technological opportunities

Methods for Evaluating the Content, Usability, and Efficacy of Commercial Mobile Health Apps

Commercial mobile apps for health behavior change are flourishing in the marketplace, but little evidence exists to support their use. This paper summarizes methods for evaluating the content, usability, and efficacy of commercially available health apps. Content analyses can be used to compare app features with clinical guidelines, evidence-based protocols, and behavior change techniques. Usability testing can establish how well an app functions and serves its intended purpose for a target population. Observational studies can explore the association between use and clinical and behavioral outcomes. Finally, efficacy testing can establish whether a commercial app impacts an outcome of interest via a variety of study designs, including randomized trials, multiphase optimization studies, and N-of-1 studies. Evidence in all these forms would increase adoption of commercial apps in clinical practice, inform the development of the next generation of apps, and ultimately increase the impact of commercial apps. Boudreaux, Rajani S Sadasivam, Sean P Mullen, Jennifer L Carey, Rashelle B Hayes, Eric Y Ding, Gary G Bennett, Sherry L Pagoto. Originally published in JMIR Mhealth and Uhealth (http://mhealth.jmir.org), 18.12.2017

Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling.

Intracellular signals triggered by DNA breakage flow through proteins containing BRCT (BRCA1 C-terminal) domains. This family, comprising 23 conserved phosphopeptide-binding modules in man, is inaccessible to small-molecule chemical inhibitors. Here, we develop Bractoppin, a drug-like inhibitor of phosphopeptide recognition by the human BRCA1 tandem (t)BRCT domain, which selectively inhibits substrate binding with nanomolar potency in vitro. Structure-activity exploration suggests that Bractoppin engages BRCA1 tBRCT residues recognizing pSer in the consensus motif, pSer-Pro-Thr-Phe, plus an abutting hydrophobic pocket that is distinct in structurally related BRCT domains, conferring selectivity. In cells, Bractoppin inhibits substrate recognition detected by Förster resonance energy transfer, and diminishes BRCA1 recruitment to DNA breaks, in turn suppressing damage-induced G2 arrest and assembly of the recombinase, RAD51. But damage-induced MDC1 recruitment, single-stranded DNA (ssDNA) generation, and TOPBP1 recruitment remain unaffected. Thus, an inhibitor of phosphopeptide recognition selectively interrupts BRCA1 tBRCT-dependent signals evoked by DNA damage

Role of matrix metalloproteinases in multi-system inflammatory syndrome and acute COVID-19 in children

INTRODUCTION: Multisystem Inflammatory Syndrome in children (MIS-C) is a serious inflammatory sequela of SARS-CoV2 infection. The pathogenesis of MIS-C is vague and matrix metalloproteinases (MMPs) may have an important role. Matrix metalloproteinases (MMPs) are known drivers of lung pathology in many diseases. METHODS: To elucidate the role of MMPs in pathogenesis of pediatric COVID-19, we examined their plasma levels in MIS-C and acute COVID-19 children and compared them to convalescent COVID-19 and children with other common tropical diseases (with overlapping clinical manifestations). RESULTS: Children with MIS-C had elevated levels of MMPs (P < 0.005 statistically significant) in comparison to acute COVID-19, other tropical diseases (Dengue fever, typhoid fever, and scrub typhus fever) and convalescent COVID-19 children. PCA and ROC analysis (sensitivity 84–100% and specificity 80–100%) showed that MMP-8, 12, 13 could help distinguish MIS-C from acute COVID-19 and other tropical diseases with high sensitivity and specificity. Among MIS-C children, elevated levels of MMPs were seen in children requiring intensive care unit admission as compared to children not needing intensive care. Similar findings were noted when children with severe/moderate COVID-19 were compared to children with mild COVID-19. Finally, MMP levels exhibited significant correlation with laboratory parameters, including lymphocyte counts, CRP, D-dimer, Ferritin and Sodium levels. DISCUSSION: Our findings suggest that MMPs play a pivotal role in the pathogenesis of MIS-C and COVID-19 in children and may help distinguish MIS-C from other conditions with overlapping clinical presentation