2.8-Å Structure of penicillin-sensitive D-alanyl carboxypeptidase-transpeptidase from Streptomyces R61 and complexes with β-lactams

The crystallographic structure of the penicillin-sensitive D-alanyl carboxypeptidase-transpeptidase from Streptomyces R61 has been solved to 2.8-A resolution. The 38,000-dalton serine peptidase has two regions of secondary structure, an alpha/beta cluster, and a region which contains five helical segments. The beta sheet is composed of five beta strands. The tertiary structure has no homology with the classic serine proteases or with the zinc carboxypeptidases. The binding at a common site of three types of beta-lactam (a penicillin, a cephalosporin, a monocyclic beta-lactam) and a desazacyclobutanone has been observed in Fourier difference maps. The binding site sequence is Val-Gly-Ser-Val-Thr-Lys. The beta-lactam ring lies near the enzyme's catalytic serine at position 37, and the C3 substituent of a cephalosporin falls near lysine 40

ELEPHANT: ExtragaLactic alErt Pipeline for Hostless AstroNomical Transients

International audienceContext. Transient astronomical events that exhibit no discernible association with a host galaxy are commonly referred to as hostless. These rare phenomena are associated with extremely energetic events, and they can offer unique insights into the properties and evolution of stars and galaxies. However, the sheer number of transients captured by contemporary high-cadence astronomical surveys renders the manual identification of all potential hostless transients impractical. Therefore, creating a systematic identification tool is crucial for studying these elusive events. Aims. We present the ExtragaLactic alErt Pipeline for Hostless AstroNomical Transients (ELEPHANT), a framework for filtering hostless transients in astronomical data streams. Methods. We used Fink to access all the ZTF alerts produced between January/2022 and December/2023, selecting only those associated with extragalactic transients. We then processed the associated stamps using a sequence of image analysis techniques to retrieve hostless candidates. Results. We find that less than 2% of all analyzed transients are potentially hostless. Among them, approximately 10% have a spectroscopic class reported on TNS, with Type Ia supernova being the most common class, followed by SLSN. Among the hostless candidates retrieved by our pipeline, there was SN 2018ibb, which has been proposed to be a PISN candidate; and SN 2022ann, one of only five known SNe Icn. When no class is reported on TNS, the dominant classes are QSO and SN candidates, the former obtained from SIMBAD and the latter inferred using the Fink ML classifier. Conclusions. ELEPHANT represents an effective strategy to filter extragalactic events within large and complex astronomical alert streams. There are many applications for which this pipeline will be useful, ranging from transient selection for follow-up to studies of transient environments

ELEPHANT: ExtragaLactic alErt Pipeline for Hostless AstroNomical Transients

International audienceContext. Transient astronomical events that exhibit no discernible association with a host galaxy are commonly referred to as hostless. These rare phenomena are associated with extremely energetic events, and they can offer unique insights into the properties and evolution of stars and galaxies. However, the sheer number of transients captured by contemporary high-cadence astronomical surveys renders the manual identification of all potential hostless transients impractical. Therefore, creating a systematic identification tool is crucial for studying these elusive events. Aims. We present the ExtragaLactic alErt Pipeline for Hostless AstroNomical Transients (ELEPHANT), a framework for filtering hostless transients in astronomical data streams. Methods. We used Fink to access all the ZTF alerts produced between January/2022 and December/2023, selecting only those associated with extragalactic transients. We then processed the associated stamps using a sequence of image analysis techniques to retrieve hostless candidates. Results. We find that less than 2% of all analyzed transients are potentially hostless. Among them, approximately 10% have a spectroscopic class reported on TNS, with Type Ia supernova being the most common class, followed by SLSN. Among the hostless candidates retrieved by our pipeline, there was SN 2018ibb, which has been proposed to be a PISN candidate; and SN 2022ann, one of only five known SNe Icn. When no class is reported on TNS, the dominant classes are QSO and SN candidates, the former obtained from SIMBAD and the latter inferred using the Fink ML classifier. Conclusions. ELEPHANT represents an effective strategy to filter extragalactic events within large and complex astronomical alert streams. There are many applications for which this pipeline will be useful, ranging from transient selection for follow-up to studies of transient environments

Gaia DR2 unravels incompleteness of nearby cluster population: new open clusters in the direction of Perseus

International audienceContext. Open clusters (OCs) are popular tracers of the structure and evolutionary history of the Galactic disc. The OC population is often considered to be complete within 1.8 kpc of the Sun. The recent Gaia Data Release 2 (DR2) allows the latter claim to be challenged.Aims. We perform a systematic search for new OCs in the direction of Perseus using precise and accurate astrometry from Gaia DR2.Methods. We implemented a coarse-to-fine search method. First, we exploited spatial proximity using a fast density-aware partitioning of the sky via a k-d tree in the spatial domain of Galactic coordinates, (l, b). Secondly, we employed a Gaussian mixture model in the proper motion space to tag fields quickly around OC candidates. Thirdly, we applied an unsupervised membership assignment method, UPMASK, to scrutinise the candidates. We visually inspected colour-magnitude diagrams to validate the detected objects. Finally, we performed a diagnostic to quantify the significance of each identified over-density in proper motion and in parallax space.Results. We report the discovery of 41 new stellar clusters. This represents an increment of at least 20% of the previously known OC population in this volume of the Milky Way. We also report on the clear identification of NGC 886, an object previously considered an asterism. This study challenges the previous claim of a near-complete sample of OCs up to 1.8 kpc. Our results reveal that this claim requires revision, and a complete census of nearby OCs is yet to be found.Key words: open clusters and associations: general / methods: numerical★ The list of members are only available at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/624/A12