Stimuli-Responsive Multilayers Based on Thiolated Polysaccharides That Affect Fibroblast Cell Adhesion

Control of the biomaterial properties through stimuli-responsive polymeric platforms has become an essential technique in recent biomedical applications. A multilayer system of thiolated chitosan (t-Chi) and thiolated chondroitin sulfate (t-CS), consisting of five double layers ([t-Chi/t-CS]5), was fabricated here by applying a layer-by-layer coating strategy. To represent a novel class of chemically tunable nanostructures, the ability to cross-link pendant thiol groups was tested by a rise from pH 4 during layer formation to pH 9.3 and a more powerful chemical stimulus by using chloramine-T (ChT). Following both treatments, the resulting multilayers showed stimuli-dependent behavior, as demonstrated by their content of free thiols, wettability, surface charge, elastic modulus, roughness, topography, thickness, and binding of fibronectin. Studies with human dermal fibroblasts further demonstrated the favorable potential of the ChT-responsive multilayers as a cell-adhesive surface compared to pH-induced cross-linking. Because the [t-Chi/t-CS]5 multilayer system is responsive to stimuli such as the pH and redox environment, multilayer systems with disulfide bond formation may help to tailor their interaction with cells, film degradation, and controlled release of bioactive substances like growth factors in a stimuli-responsive manner useful in future wound healing and tissue engineering applications

Gewaltopferakte and GOASSIST-App for victims of violence experiences - an integrated digital solution for the care of victims of violence

Background: The comprehensive care of victims of violence is still insufficient, even though physical, psychological and sexual violence, especially in the domestic environment, is an omnipresent problem. The care of victims of violence requires a high degree of communication and coordination between practitioners from different professions and those affected. Digital applications can provide support in this regard. Method: As part of a work package of the EFRE-funded research project iGOBSIS-pro, an integrated digital solution for violence victim care was developed. Results: The resulting "Gewaltopferakte" (victims of violence file) as a documentation platform for health professionals as well as the GOASSIST-App for victims can ensure a holistic care structure for victims of violence and thus also psychological aftercare following medical treatment and evidence recovery. Discussion and conclusion: The inter-professional software solution overcomes the fragmentation in the care of victims of violence and thus makes an important contribution to the comprehensive care. Further evaluations are necessary.Hintergrund: Die flächendeckende Gewaltbetroffenenversorgung ist noch immer unzureichend und das, obwohl physische, psychische und sexuelle Gewalt, vor allem im häuslichen Umfeld, nach wie vor ein allgegenwärtiges Problem darstellt. Die Gewaltbetroffenenversorgung erfordert ein hohes Maß an Kommunikation und Abstimmung zwischen Behandler*innen verschiedener Professionen und den Betroffenen. Hierbei können digitale Anwendungen eine Unterstützung darstellen. Methode: Im Rahmen eines Arbeitspaketes des EFRE-finanzierten Projektes iGOBSIS-pro wurde eine integrierte Digitallösung für die Gewaltbetroffenenversorgung entwickelt. Ergebnisse: Die resultierende Gewaltopferakte als Dokumentationsplattform für Heilberufler*innen sowie die GOASSIST-App für Betroffene können eine ganzheitliche Versorgungsstruktur für Gewaltbetroffene und damit auch die psychologische Nachsorge nach einer medizinischen Behandlung und Spurensicherung gewährleisten.Diskussion und Schlussfolgerung: Die professionsübergreifende Softwarelösung überwindet die Fragmentierung in der Arbeit mit Gewaltbetroffenen und leistet damit einen wichtigen Beitrag zur flächendeckenden Gewaltbetroffenenversorgung. Weitere Evaluationen sind notwendig