Heuristics in permutation GOMEA for solving the permutation flowshop scheduling problem

The recently introduced permutation Gene-pool Optimal Mixing Evolutionary Algorithm (GOMEA) has shown to be an effective Model Based Evolutionary Algorithm (MBEA) for permutation problems. So far, permutation GOMEA has only been used in the context of Black-Box Optimization (BBO). This paper first shows that permutation GOMEA can be improved by incorporating a constructive heuristic to seed the initial population. Secondly, the paper shows that hybridizing with job swapping neighborhood search does not lead to consistent improvement. The seeded permutation GOMEA is compared to a state-of-the-art algorithm (VNS4) for solving the Permutation Flowshop Scheduling Problem (PFSP). Both unstructured and structured instances are used in the benchmarks. The results show that permutation GOMEA often outperforms the VNS4 algorithm for the PFSP with the total flowtime criterion

Stock status and potential yield of deep water rose shrimp (Parapenaeus longirostris, Lucas 1846) in the south-central Mediterranean Sea

This document summarises the data used, methods adopted and the results of the joint stock assessment carried out during the MedSudMed and CopeMed II Working Groups on Parapenaeus longirostris and related fisheries held in 2009 and 2010 in Malta and Italy respectively. The aim of this document is to describe the state of the stock of P. longirostris in the south-central Mediterranean Sea using data from Italy, Tunisia and Malta. The long term objective of this study is to provide the baseline for the establishment of a harmonised management regime for the deep water rose shrimp fishery in the south-central Mediterranean Sea. Overall, fishery dependent data from Italy, Malta and Tunisia for the first time were pooled together and jointly processed. The assessment was performed using length cohort analysis (LCA) and biomass and yield per recruits analyses as implemented in VIT4Win. Current mean fishing mortality and exploitation pattern were assessed using the steady state LCA on length frequency distributions (LFD) of 2007, 2008 and 2009 as well as the average 2007-2009 catches, raised to the total landings. Analyses were performed separately by keeping sex and fleet segments separate (i.e. Italy 12-24; Italy > 24; Tunisia; Malta) as first step. A sensitivity analysis was also carried out as implemented in VIT. The results are discussed in the view of providing robust technical advice for the management of national fisheries exploiting P. longirostris in the MedSudMed area taking into account the possible evolution of national fisheries. Some perspective for future joint activities to be carried out within the cooperative framework established by the FAO Projects are also presented.peer-reviewe

Seascape connectivity of European anchovy in the Central Mediterranean Sea revealed by weighted Lagrangian backtracking and bio-energetic modelling

Ecological connectivity is one of the most important processes that shape marine populations and ecosystems, determining their distribution, persistence, and productivity. Here we use the synergy of Lagrangian back-trajectories, otolith-derived ages of larvae, and satellite-based chlorophyll-a to identify spawning areas of European anchovy from ichthyoplanktonic data, collected in the Strait of Sicily (Central Mediterranean Sea), i.e., the crucial channel in between the European and African continents. We obtain new evidence of ecosystem connectivity between North Africa and recruitment regions off the southern European coasts. We assess this result by using bio-energetic modeling, which predicts species-specific responses to environmental changes by producing quantitative information on functional traits. Our work gives support to a collaborative and harmonized use of Geographical Sub-Areas, currently identified by the General Fisheries Commission for the Mediterranean. It also confirms the need to incorporate climate and environmental variability effects into future marine resources management plans, strategies, and directives

A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1

BACKGROUND: Previously we described a transdominant negative mutant of the HIV-1 Tat protein, termed Nullbasic, that downregulated the steady state levels of unspliced and singly spliced viral mRNA, an activity caused by inhibition of HIV-1 Rev activity. Nullbasic also altered the subcellular localizations of Rev and other cellular proteins, including CRM1, B23 and C23 in a Rev-dependent manner, suggesting that Nullbasic may disrupt Rev function and trafficking by intervening with an unidentified component of the Rev nucleocytoplasmic transport complex. RESULTS: To seek a possible mechanism that could explain how Nullbasic inhibits Rev activity, we used a proteomics approach to identify host cellular proteins that interact with Nullbasic. Forty-six Nullbasic-binding proteins were identified by mass spectrometry including the DEAD-box RNA helicase, DDX1. To determine the effect of DDX1 on Nullbasic-mediated Rev activity, we performed cell-based immunoprecipitation assays, Rev reporter assays and bio-layer interferometry (BLI) assays. Interaction between DDX1 and Nullbasic was observed by co-immunoprecipitation of Nullbasic with endogenous DDX1 from cell lysates. BLI assays showed a direct interaction between Nullbasic and DDX1. Nullbasic affected DDX1 subcellular distribution in a Rev-independent manner. Interestingly overexpression of DDX1 in cells not only restored Rev-dependent mRNA export and gene expression in a Rev reporter assay but also partly reversed Nullbasic-induced Rev subcellular mislocalization. Moreover, HIV-1 wild type Tat co-immunoprecipitated with DDX1 and overexpression of Tat could rescue the unspliced viral mRNA levels inhibited by Nullbasic in HIV-1 expressing cells. CONCLUSIONS: Nullbasic was used to further define the complex mechanisms involved in the Rev-dependent nuclear export of the 9 kb and 4 kb viral RNAs. All together, these data indicate that DDX1 can be sequestered by Nullbasic leading to destabilization of the Rev nucleocytoplasmic transport complex and decreased levels of Rev-dependent viral transcripts. The outcomes support a role for DDX1 in maintenance of a Rev nuclear complex that transports viral RRE-containing mRNA to the cytoplasm. To our knowledge Nullbasic is the first anti-HIV protein that specifically targets the cellular protein DDX1 to block Rev’s activity. Furthermore, our research raises the possibility that wild type Tat may play a previously unrecognized but very important role in Rev function. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12977-014-0121-9) contains supplementary material, which is available to authorized users

A hybrid genetic algorithm for solving no-wait flowshop scheduling problems

International audienc

Métaheuristiques pour l'ordonnancement multicritère et les problèmes de transport

International audienceno abstrac

Metaheuristics for Production Scheduling

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A hybrid genetic algorithm for solving no‐wait flowshop scheduling problems

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Métaheuristiques pour l'ordonnancement monocritère des ateliers de production

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