Condições hídricas do Estado da Paraíba sob diferentes cenários do tempo.

Considerando-se os cenários futuros projetados por vários modelos de simulação, as regiões semiáridas deverão ser afetadas pelas mudanças do clima. Dados climáticos históricos foram usados em conjunto com projeções através do modelo HadCM3 do centro Hadley, na determinação de componentes do balanço hídrico climático, permitindo a quantificação da deficiência hídrica, para avaliação da variação nas disponibilidades mensais e anuais de água no Estado da Paraíba, considerando-se as condições médias da linha base de 1961-1990 e os cenários do IPCC para 2020, 2050 e 2080. De acordo com estas projeções, constatou-se que reduções das disponibilidades hídricas mais elevadas irão ocorrer no primeiro semestre do ano com valores médios de deficiência acima de 200 mm no mês de abril para o ano projetado de 2080, sendo as áreas do oeste do estado as mais afetadas pela escassez de água

The opportunity for smart city projects at municipal scale: Implementing a positive energy district in Zorrozaurre

The urgency of climate change is demanding new urban energy transition processes that will be accelerated by the implementation of innovative urban solutions. This paper proposes a three-step methodology to encompass the energy transition in cities. Firstly, the design of urban spaces in accordance to Positive Energy District (PED) concept is defining a very ambitious objective that will lead the development and implementation of innovative urban approaches. Secondly, the implementation of Urban City Labs is proposed for testing and demonstrating urban innovations at real scale as reasonable approach for consolidated urban landscapes. Thirdly, energy transition is demanding new governance mechanisms where top-down and bottom-up perspectives are continually combined and harmonized. ATELIER H2020 is accelerating the demonstration of this methodology at the recently defined PED in Zorrozaurre (Bilbao, Basque Country)

Physiological, morphological characteristics and blood profile of female elite Brazilian soccer players according to position

The main goal of this study was to identify the performance parameters and blood profile found among female soccer players. The second aim was to examine if there were any systematic differences between players assigned to different playing positions. Thirty-six players (age: 23.7 ± 3.5 y; weight: 61.9 ± 5.7 kg; height: 165.7± 6.2 cm) underwent a set of laboratory tests (cardiopulmonary exercise test, skinfold measurements, Wingate test, sit-and-reach test, and blood biochemical tests). The following results were verified: maximal oxygen uptake (53±3.9 mL.kg-1.min-1), second ventilatory threshold (11.5 ± 0.8 km.h-1), body fat (14.1 ± 2.9%), Wingate anaerobic test (peak power: 9.5 ± 0.8 w.kg-1; mean power: 7.3 ± 0.4 w.kg-1 and fatigue index: 55.5 ± 4.9%),  flexibility test [sit-and-reach]  (18.1 ± 2.9 cm) and biochemical parameters of blood (Hb:13.9 ± 0.3 g.dL-1; iron: 85.2 ± 12.6 µ.dL-1; calcium: 9.2±0.5 mg.dL-1;  total cholesterol: 204.7 ± 34.7 mg.dL-1; HDL-c: 50.7± 3.6 mg.dL-1; LDL-c: 125.8± 23.3 mg.dL-1; triglycerides: 96.8±18.5 mg.dL-1). In conclusion, the current results indicate that present elite players' physiological characteristics are similar to those previously shown, despite the rapid changes of the female soccer game worldwide. However, data showed that different playing positions had different physiological and anthropometrics differences.

Stomach contents of sperm whales Physeter macrocephalus stranded in the North Sea 1990-1996

Stomach contents of 17 sperm whales Physeter macrocephalus stranded in Scotland and Denmark during 1990-96 were analysed. All were sub-adult or adult males and stranded between November and March. They had presumably entered the North Sea during their southward migration from feeding grounds in Arctic waters. Other studies indicate that the majority of the whales were apparently healthy. The diet of these whales was found to consist almost entirely of cephalopods, principally squid of the genus Gonatus (hereafter 'Gonatus', but probably G. fabricii, an oceanic species characteristic of Arctic waters). The other prey species identified were also mostly oceanic cephalopods: the squids Histioteuthis bonnellii, Teuthowenia megalops and Todarodes sagittatus and the octopus Haliphron atlanticus. Although these results are consistent with other recent studies in the area based on single stranded whales, they differ from results of work on whales caught during commercial whaling operations in Icelandic waters (1960s to 1980s) in that little evidence of predation on fish was found in the present study. Remains of single individuals of the veined squid Loligo forbesi, the northern octopus Eledone cirrhosa and the saithe Pollachius virens provided the only possible evidence of feeding in the North Sea. We infer that sperm whales do not enter the North Sea to feed. The timing, and large and uniform sizes of the Gonatus species eaten (most had mantle lengths in the range 195 to 245 mm), as estimated from measurements of the lower beaks, and the seasonality of the strandings is consistent with the whales having fed on mature squid, possibly spawning concentrations--as has recently been reported for bottlenose whales. Assuming that the diet recorded in this study was representative of sperm whales during the feeding season, as much as 500000 t of Gonatus could be removed by sperm whales in Norwegian waters each year and up to 3 times that figure from the eastern North Atlantic as a whole. Evidence from other studies indicates that Gonatus is an important food resource for a wide range of marine predators in Arctic waters

Dissecting the role of TP53 alterations in del(11q) chronic lymphocytic leukemia

© 2021 The Authors.[Background]: Several genetic alterations have been identified as driver events in chronic lymphocytic leukemia (CLL) pathogenesis and oncogenic evolution. Concurrent driver alterations usually coexist within the same tumoral clone, but how the cooperation of multiple genomic abnormalities contributes to disease progression remains poorly understood. Specifically, the biological and clinical consequences of concurrent high-risk alterations such as del(11q)/ATM-mutations and del(17p)/TP53-mutations have not been established.[Methods]: We integrated next-generation sequencing (NGS) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 techniques to characterize the in vitro and in vivo effects of concurrent monoallelic or biallelic ATM and/or TP53 alterations in CLL prognosis, clonal evolution, and therapy response.[Results]: Targeted sequencing analysis of the co-occurrence of high-risk alterations in 271 CLLs revealed that biallelic inactivation of both ATM and TP53 was mutually exclusive, whereas monoallelic del(11q) and TP53 alterations significantly co-occurred in a subset of CLL patients with a highly adverse clinical outcome. We determined the biological effects of combined del(11q), ATM and/or TP53 mutations in CRISPR/Cas9-edited CLL cell lines. Our results showed that the combination of monoallelic del(11q) and TP53 mutations in CLL cells led to a clonal advantage in vitro and in in vivo clonal competition experiments, whereas CLL cells harboring biallelic ATM and TP53 loss failed to compete in in vivo xenotransplants. Furthermore, we demonstrated that CLL cell lines harboring del(11q) and TP53 mutations show only partial responses to B cell receptor signaling inhibitors, but may potentially benefit from ATR inhibition.[Conclusions]: Our work highlights that combined monoallelic del(11q) and TP53 alterations coordinately contribute to clonal advantage and shorter overall survival in CLL.Spanish Fondo de Investigaciones Sanitarias, Grant/Award Numbers: PI15/01471, PI18/01500); Fundación Memoria Don Samuel Solórzano Barruso, Grant/Award Number: RD12/0036/006