Development of cryopreservation for Loxocarya cinerea - an endemic Australian plant species important for post-mining restoration

We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 ± 5.9%; SXH804, 67.7 ± 9.6%). Extension of incubation in PVS2 from 30 to 60 min did not lead to survival after cryopreservation. Thermal analysis using differential scanning calorimetry confirmed the beneficial effect of a loading phase but also revealed no or very little ice formation after cryoprotection of shoot tips in other treatments. Regeneration following cryopreservation was obtained for genotype SXH804 (4.3 ± 2.1%) but not for SXH404. Regenerated explants of L. cinerea SXH804 were morphologically identical to tissue-cultured plants. As an alternative to shoot tips, callus tissues of clone SXH404 were successfully cryopreserved (>66.7% post LN survival) using the same protocol

De Nederlandse geschiedenis is in de negentiende eeuw verzonnen. Interview met Maria Grever, Pieter Huistra, James Kennedy en Peter Raedts

De huidige strijd over de Nederlandse geschiedenis is niets nieuws. Het begon allemaal met gekrakeel van historici tweehonderd jaar geleden

De Nederlandse geschiedenis is in de negentiende eeuw verzonnen. Interview met Maria Grever, Pieter Huistra, James Kennedy en Peter Raedts

De huidige strijd over de Nederlandse geschiedenis is niets nieuws. Het begon allemaal met gekrakeel van historici tweehonderd jaar geleden

'Deze moslima's zijn vooral voetballer'

Contains fulltext : 209633pub.pdf (publisher's version ) (Open Access)Meisjes die mee willen doen aan pleintjesvoetbal worden niet zomaar toegelaten door de jongens. Ze worden ook anders gecoacht.17 mei 201

'Deze moslima's zijn vooral voetballer'

Meisjes die mee willen doen aan pleintjesvoetbal worden niet zomaar toegelaten door de jongens. Ze worden ook anders gecoacht

Influence of abiotic stress preconditioning on antioxidant enzymes in shoot tips of Lomandra sonderi (Asparagaceae) prior to cryostorage

Lomandra sonderi (F.Muell.) Ewart (Asparagaceae) is endemic to the south-west Western Australian jarrah (Eucalyptus marginata Donn ex Sm.) forest region, and is a difficult to propagate species important to post-mining restoration. Micropropagation is the only way to currently produce plants of this species for restoration. This study describes investigations into optimising cryopreservation for efficient long-term germplasm storage. In order to investigate the effect of preconditioning on post-cryogenic survival of shoot tips, in vitro grown plants were exposed to a range of light-, temperature- and osmotic-induced preconditioning treatments under culture room conditions for 3 weeks. Room temperature (24°C) preconditioning resulted in the greatest post-cryogenic survival, followed by low light (17 µmol m-2 s-1) preconditioning. Alternating temperature (25/5°C), high temperature (35°C), high sucrose (180mM) and high light (93 µmol m-2 s-1) preconditioning treatments all led to significantly and progressively lower post-cryogenic shoot tip survival than room temperature preconditioning. Antioxidant activity of superoxide dismutase in preconditioned shoot tips showed a positive correlation to post-cryogenic survival overall, whereas the activities of glutathione reductase, glutathione peroxidase and catalase showed little correlation. Analysis throughout the cryopreservation protocol showed that the activity of glutathione reductase decreased significantly after cryopreservation, whilst the activity of glutathione peroxidase and catalase did not change

Evaluation of the new vacuum infiltration vitrification (viv) cryopreservation technique for native Australian plant shoot tips

BACKGROUND: The application of a vacuum during the incubation in cryoprotective agents such as PVS2 allows for increased penetration, reducing total incubation times required before vitrification and post-cryopreservation regeneration is achieved. OBJECTIVE: This study compared a conventional droplet-vitrification protocol to the new vacuum infiltration vitrification protocol in four Australian plant species. MATERIALS AND METHODS: The new vacuum infiltration vitrification applied an 80 kPa vacuum during incubations in loading solution and PVS2. Infiltration of the cryoprotective agents into shoot tips was determined by differential scanning calorimetry measuring ice formation in the thermographs comparing a range of loading solution and PVS2 incubation times. RESULTS AND CONCLUSION: The application of the vacuum infiltration vitrification technique resulted in a significantly reduced PVS2 incubation time for cryogenic survival and regeneration for all four species, reducing the time needed to adequately protect shoot tips by half to a quarter when compared to a conventional droplet-vitrification technique